PYROSEQUENCING
SAMPLE INFORMATION FORM
Albert Einstein College of Medicine
713 Ullmann, x2657
GENOTYPE@AECOM.YU.EDU
Director: David Reynolds DREYNOLD@AECOM.YU.EDU
www.aecom.yu.edu/dna
Investigator information
Please provide 10l of PCR product in 0.2 ml strip tubes or 96well plates with sequencing primer in a separate tube
(0.5l of 10M primer per reaction.)
See website for PCR conditions.
Principle Investigator
Department
Date Submitted
This form should be dropped off with samples at Ullmann 713
and an electronic sample form should be submitted through
the reststop folder: \\reststop\public\genotype
A blank e-sample form is available at
www.aecom.yu.edu/dna
Contact Person
Telephone Number
E-Mail Address
Rm/Bldg Address
Grant #
Sample submission
9 - 526 -
- 431
Sequence of PCR product and assay design output
from pyrosequencing website should also be submitted to the
reststop folder for each new assay.
QUANTIFICATION: You must check your PCR product on a gel
to ensure a clear, strong band without haze or artifact.
PYROSEQUENCING RESULTS: The facility provides a Word
file. Notification will be sent by e-mail and results will be available
on reststop.
Indicate Folder:________________________________________
Special Request: ___________________________________
For primer design website go to
http://techsupport.pyrosequencing.com
Register using serial # 60-0110-000111
and software # 50-0176
to obtain customer and user ID.
Please visit our website for help with assay design.
Sample information:
Number of Samples:_______________________ Number of sequencing primers:___________
Sample Name
Assay
Sequencing Primer
Submissions of greater than 10 samples should include an electronic sample list as described above.
Comments:
Facility Input