The CWRU Genomics Core Facility
Sanger Sequencing Request Form
Simone Edelheit, Manager
BRB 747A
368-1887
sxw94@case.edu
Date: ______________________________________
Your Name: _________________________________
Primary Investigator: __________________________
Email: ______________________________________
Department: _________________________________ Account Number: _____________________________
Phone Number: ______________________________
Affiliation:
Project Title: ________________________________
Funding:
CWRU
NIH
CCCC
NSF
CTSC
CCF
OTHER
OTHER
Sample Submission:



Please bring DNA and primers in SEPARATE tubes labeled with sample name/primer, date, and your name.
Make sure to list an annealing temperature for your primers. Submit primers @ 20uM concentration.
For PCR products that require EXO/SAP, provide a minimum of 10ul. EXO/SAP clean-up is $1/ sample.
Type of Template:
Plasmid
Short PCR product (<500bp)
Long PCR product (>500bp)
BAC
Primer
#
Sample Name
Quantity:
100-300ng per reaction
50-100ng per reaction
100-200ng per reaction
1ug per reaction
2ul of 20uM concentration per reaction
Primer
(20µM)
DNA quantity
(ng/µl)
Is PCR product
clean?
Annealing temp.,
special conditions, etc.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
Sequencing Service:
# reactions ________ @ $8.00/rxn
EXO/SAP service:
# reactions _______ @ $1.00/rxn
Total:
Full plate (96 or more)
# reactions ________ @ $6.00/rxn
Sanger Sequencing Request Form, updated July 2015