ISEM SOP TEMPLATE - UCI Environmental Health & Safety

Name of Work Unit:
Standard Operating Procedures for 2-Chloroethyl Ethyl Sulfide (CEES, Half Mustard Gas) (CAS# 693-07-2)
Please write
ideas in space
Scope of Work/Activity: State the process/operation/equipment that the SOP concerns.
Topical application of CEES to tissue culture cells or oral surfaces in animals
Specific Safety and Environmental Hazards: State the specific hazard and
consequences if procedure not followed to person, environment, or property.
Harmful by inhalation, skin contact or swallowing. Possible mutagen. Toxic. Causes
blisters on contact with skin.
Engineering Controls: Describe any specific engineering controls which are required to
prevent employee injury to hazards such mechanical lifts, automatic sensors, machine
Perform all work inside an approved fume hood. Work 6” back from fume hood mouth.
Flow at hood mouth should be at least 100 linear feet per minute.
Designated Area: Indicate the designated area for performing this process.
Fume hoods in ______________.
Personal Protective Equipment (PPE): State the personal protective equipment selected and
required. Examples: safety spectacles, work gloves, steel toe shoes.
Lab coats; safety goggles; double glove with nitrile gloves over latex-band latex at wrist over lab coat
sleeve. Gloves should be changed hourly if work takes longer than 60 minutes.
Important Steps to Follow: List the specific sequence staff should follow to avoid
1. The fume hood should be checked for flow rate within 24 hours of expected use.
2. Gloves should be inspected for holes or tears.
3. The double layered container with the stock CEES should be opened only inside
the fume hood. Once the desired amount of stock material has been removed, the
container should be resealed and returned to the refrigerator where stored.
4. All containers with CEES solutions should be kept capped any time not being
actively manipulated.
5. If tissue culture dishes/flasks are used they should be placed on an impermeable
tray for secondary containment.
6. If animals are used they should be fully anesthetized before being placed in the
hood for exposure and inside a secondary containment apparatus lined with absorbent
material backed with plastic.
7. After use all contaminated materials should be immediately placed in the
appropriate waste receptacles placed inside the hood and kept inside the hood until
picked up by EH&S. Liquid waste should not be placed in glass containers. All waste
should be double bagged.
UC Irvine Environmental Health & Safety, SOP Library, 2/16/2016
Accident Procedures: Describe any special procedures staff should follow in case of
Spill: Small—wipe up with absorbent material, wash area with water, place
contaminated materials in appropriate waste containers
Spill: Large—Cover area with absorbent material, close hood sash, contact EH&S
Outer Glove contact: Remove outer gloves, place in disposal container inside hood,
replace with new gloves.
Clothing: Remove immediately, follow directions for skin contact
Skin contact: Wash with soap and water, rinse for at least 15 minutes, seek medical
advice if reaction seen
Eye contact: Flush with water for at least 15 minutes, seek medical advice immediately
Mouth contact: Rinse mouth with water (if conscious), seek medical advice
Inhalation: Remove to fresh air. Give oxygen if breathing is difficult, if not breathing
give artificial respiration. Seek immediate medical treatment.
Training & Competency Requirements: Describe necessary training and demonstration of
competency for performing the hazardous operation.
To be handled only by permanent, supervisory research staff with documented laboratory safety
Approval Required: Indicate if and when an approval form the Supervisor is required.
Not required since only supervisors will be handling the materials.
SOP Review Frequency: Specify how often the SOP is reviewed for effectiveness.
Before each experiment.
Training Acknowledgement: I have read, asked questions, and understand the hazards of and safe working
procedures for the activity/materials described herein.
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UC Irvine Environmental Health & Safety, SOP Library, 2/16/2016