biomaterial: 832/1 INS1 Cells---id 5005---Poorly Glucose Responsive
bio-characteristic
 CellType→insulinoma
 OrganismPart(islet of Langerhans)→islet
 CellLine(Poorly glucose responsive insulinoma cell line)→user_defined→832/1 INS1
Line 834/40 → lines 833/15 and 833/117
We therefore treated clonal cell lines derived from INS-1res cells (lines 833/15 and 833/117) or
parental INS-1 cells (line 834/40) acutely with IFN-γ or IL-1β + IFN-γ for a period of 20 minutes, and
then assayed phosphorylation and expression of STAT1α by immunoblot analysis.1
Cell Lines.

INS-1 and INS-2
New insulin-secreting cell lines (INS-1 and INS-2) were established from cells
isolated from an x-ray-induced rat transplantable insulinoma. The continuous growth
of these cells was found to be dependent on the reducing agent 2-mercaptoethanol.
Removal of this thiol compound caused a 15-fold drop in total cellular glutathione
levels. These cells proliferated slowly (population doubling time about 100 h) and,
in general, showed morphological characteristics typical of native beta-cells. Most
cells stained positive for insulin and did not react with antibodies against the
other islet hormones. The content of immunoreactive insulin was about 8
micrograms/10(6) cells, corresponding to 20% of the native beta-cell content. These
cells synthesized both proinsulin I and II and displayed conversion rates of the
two precursor hormones similar to those observed in rat islets. However, glucose
failed to stimulate the rate of proinsulin biosynthesis. In static incubations,
glucose stimulated insulin secretion from floating cell clusters or from attached
cells. Under perifusion conditions, 10 mM but not 1 mM glucose enhanced secretion
2.2-fold. In the presence of forskolin and 3-isobutyl-1-methylxanthine, increase of
glucose concentration from 2.8-20 mM caused a 4-fold enhancement of the rate of
secretion. Glucose also depolarized INS-1 cells and raised the concentration of
cytosolic Ca2+. This suggests that glucose is still capable of eliciting part of
the ionic events at the plasma membrane, which leads to insulin secretion. The
structural and functional characteristics of INS-1 cells remained unchanged over a
period of 2 yr (about 80 passages). Although INS-2 cells have not been fully
characterized, their insulin content was similar to that of INS-1 cells and they
also remain partially sensitive to glucose as a secretagogue. INS-1 cells retain
beta-cell surface antigens, as revealed by reactivity with the antigangloside
monoclonal antibodies R2D6 and A2B5. These findings indicate that INS-1 cells have
remained stable and retain a high degree of differentiation which should make them
a suitable model for studying various aspects of beta-cell function.
(from
Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines.
Asfari M, Janjic D, Meda P, Li G, Halban PA, Wollheim CB.
Endocrinology. 1992 Jan;130(1):167-78.
PMID: 1370150 )


833/117 and 833/15
INS-1res cells lines (833/117 and 833/15)
(From
Chen G, Hohmeier HE, Gasa R, Tran VV, Newgard CB. Diabetes. 2000 Apr;49(4):562-70. PMID: 10871193
Selection of insulinoma cell lines with resistance to interleukin-1beta- and gamma-interferon-induced cytotoxicity.
“… clonal INS-1 cell lines derived from parental cells (line 834/40) or INS-1r e s cells (lines 833/117 and 833/15).”
)
 GK2 (GKP2)
it may be a gene, glycerol kinase 2 [ Homo sapiens ] , Also known as
GKP2; GKTA.
No lit found. And also GKP4

GKP4


mPAC
Culture and transfection of mouse pancreatic ductal carcinoma (mPAC) cells and mouse βTC3,
αTC1.9, and NIH3T3 cells were performed as previously described (29).
29 Sox9 coordinates a transcriptional network in pancreatic progenitor cells. Lynn FC, Smith SB,
Wilson ME, Yang KY, Nekrep N, German MS. Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):105005. Epub 2007 Jun 11. PMID: 17563382 (mentioned, but not it)
mPAC L20
R7T1
the reversibly immortalized mouse
-cell line R7T1,
seen in
W. Wang, J. R. Walker, X. Wang et al., “Identification of small-molecule inducers of pancreatic β-cell
expansion,” Proceedings of the National Academy of Sciences of the United States of America, vol.
106, no. 5, pp. 1427–1432, 2009.
 E14TG2a
Disruption of the FII gene was accomplished in the HPRT-deficient embryonic stem (ES) cell line,
E14TG2a, which is derived from strain 129/Ola mice (21).
21
HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells.
Hooper M, Hardy K, Handyside A, Hunter S, Monk M. Nature. 1987 Mar 19-25;326(6110):292-5.
PMID: 3821905
(
FROM

αTC (a pancreatic α-cell line)
(seen in
Characterization and functional role of voltage gated cation conductances in the glucagon-like peptide1 secreting GLUTag cell line
F Reimann, M Maziarz, G Flock, AM Habib, DJ Drucker, FM Gribble
J Physiol. 2005 February 15; 563(Pt 1): 161–175. Published online 2004 December 20. doi:
10.1113/jphysiol.2004.076414 PMCID:PMC1665554

 αTC-1
The αTC-1 cell line was obtained from ATCC and was cultured in RPMI 1640 (Invitrogen) containing
10% FBS and Glutamax. TU6 cells were obtained from Marc Montminy (The Salk Institute, La Jolla,
CA) and were cultured in DMEM containing Glutamax and 10% FBS.
(Seen in:
CRFR1 is expressed on pancreatic β cells, promotes β cell proliferation, and potentiates insulin
secretion in a glucose-dependent manner
Mark O. Huising, Talitha van der Meulen, Joan M. Vaughan, Masahito Matsumoto, Cynthia J.
Donaldson, Hannah Park, Nils Billestrup, Wylie W. Vale
Proc Natl Acad Sci U S A. 2010 January 12; 107(2): 912–917. Published online 2009 December 22.
doi: 10.1073/pnas.0913610107 PMCID:PMC2818901)
αTC1 clone 9
Mouse alpha TC1/9 cells (αTC1 clone 9) were obtained from American Type Culture Collection
[(ATCC), Manassas, VA]
Differential expression of glucagon and glucagon-like peptide 1 receptors in mouse pancreatic alpha
and beta cells in two models of alpha cell hyperplasia
Mamdouh H. Kedees, Marine Grigoryan, Yelena Guz, Gladys Teitelman
Mol Cell Endocrinol. Author manuscript; available in PMC 2010 November 13.
Published in final edited form as: Mol Cell Endocrinol. 2009 November 13; 311(1-2): 69–76. Published
online 2009 July 30. doi: 10.1016/j.mce.2009.07.024 PMCID:PMC2743461
alpha TC1 clone 6 (ATCC® CRL-2934™)
ATCC® Number: CRL-2934 ™
Organism: Mus musculus, mouse
Cell Type: alpha cells
Tissue:
pancreas, alpha cells
Disease: adenoma
Product Format: frozen
For-Profit: $695.00Non-Profit: $579.17
Add to Cart
Name of Depositor
Year of Origin
References
EH Leiter
1988
Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line
derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PubMed: 2156740
Hamaguchi K, Leiter EH. Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell
lines. Viability, secretory function, and MHC antigen expression. Diabetes 39: 415-425, 1990. PubMed:
2108069
Hamaguchi K, et al. Cellular Interaction Between Mouse Pancreatic alpha-Cell and beta-Cell Lines:
Possible Contact-Dependent Inhibition of Insulin Secretion. Exp. Biol. Med. 228:1227-1233, 2003.
PubMed: 14610265
alphaTC1 Clone 9 (ATCC® CRL-2350™)
ATCC® Number: CRL-2350 ™
Organism: Mus musculus, mouse
Cell Type: alpha cell
Tissue: pancreas
Disease: adenoma
Product Format: frozen
Name of Depositor
References
EH Leiter
Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line
derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PubMed: 2156740
Hamaguchi K, Leiter EH. Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell
lines. Viability, secretory function, and MHC antigen expression. Diabetes 39: 415-425, 1990. PubMed:
2108069
transgenic for the SV40 T antigen
transgenic for the SV40 T antigen
(from
ATCC
http://www.atcc.org/Search_Results.aspx?dsNav=Ntk:PrimarySearch|pancreas%2c+alpha+cells|3|,Ny:T
rue,Ro:0,N:1000552&searchTerms=pancreas%2c+alpha+cells&redir=1
for history
alphatc-6
http://www.atcc.org/Products/All/CRL-2934.aspx#583B10CECE644CE6837044B88F427C5D
alphatc-9
http://www.atcc.org/Products/All/CRL-2350.aspx#583B10CECE644CE6837044B88F427C5D
)
The alpha TC1 line (from an adenoma created in transgenic mice expressing the SV40 large T-antigen
oncogene under control of the rat preproglucagon promoter) produced not only glucagon but also
considerable quantities of insulin (4:1 glucagon to insulin) and preproinsulin mRNA.
We therefore cloned alpha TC1 cells and obtained 12 glucagon-producing clonal cell lines that did not
produce levels of insulin detectable by radioimmunoassay. Analysis by Northern blotting of total RNA
from two lines, alpha TC1 clones 6 and 9, confirmed the absence of preproinsulin mRNA. No
somatostatin or pancreatic polypeptide was detected by immunohistochemical staining in alpha TC1
clones 6 or 9 or beta TC1 cell
(from
Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell lines. Viability, secretory
function, and MHC antigen expression.
Hamaguchi K, Leiter EH.
Diabetes. 1990 Apr;39(4):415-25. Erratum in: Diabetes 1990 Oct;39(10):1313.
PMID: 2108069
)
 CyT49
an hESC line isolated using human feeder cells under good manufacturing process (GMP) conditions
(Novocell, San Diego, CA)
(
(Novocell, San Diego, CA; Novocell Becomes ViaCyte, Inc. http://www.prnewswire.com/newsreleases/novocell-becomes-viacyte-inc-as-it-accelerates-pre-clinical-development-of-a-stem-cellderived-treatment-for-diabetes-92923104.html)
seen in
Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2
receptor signaling
Linlin Wang, Thomas C. Schulz, Eric S. Sherrer, Derek S. Dauphin, Soojung Shin, Angelique M.
Nelson, Carol B. Ware, Mei Zhan, Chao-Zhong Song, Xiaoji Chen, Sandii N. Brimble, Amanda
McLean, Maria J. Galeano, Elizabeth W. Uhl, Kevin A. D'Amour, Jonathan D. Chesnut, Mahendra S.
Rao, C. Anthony Blau, Allan J. Robins
Blood. 2007 December 1; 110(12): 4111–4119. Prepublished online 2007 August 29. doi:
10.1182/blood-2007-03-082586 PMCID: PMC2190616
 hES02 (NIH code ES02)
(
first seen in
A molecular scheme for improved characterization of human embryonic stem cell lines
Richard Josephson, Gregory Sykes, Ying Liu, Carol Ording, Weining Xu, Xianmin Zeng, Soojung
Shin, Jeanne Loring, Anirban Maitra, Mahendra S Rao, Jonathan M Auerbach
BMC Biol. 2006; 4: 28. Published online 2006 August 18. doi: 10.1186/1741-7007-4-28 PMCID:
PMC1601965
probable source article:
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Reubinoff BE, Pera
MF, Fong CY, Trounson A, Bongso A. Nat Biotechnol. 2000 Apr;18(4):399-404. Erratum in: Nat
Biotechnol 2000 May;18(5):559. PMID: 10748519 (inaccessible to jc)
)

(832/1, 832/2, 832/13, and 834/40), derived from INS-1 insulinoma β cells
Four clonal cell lines (832/1, 832/2, 832/13, and 834/40), derived from INS-1 insulinoma β cells using
a transfection-selection strategy (21, 22), were used in these studies. Cells were grown to confluence in
RPMI medium 1640 containing 11.1 mM glucose and supplemented with 10% FBS/100 units/ml
penicillin/100 μg/ml streptomycin/10 mM Hepes/2 mM glutamine/1 mM sodium pyruvate/50 μM βmercaptoethanol in 15-cm plates at 37°C in a humidified atmosphere containing 5% CO2.
21. Hohmeier, H., Mulder, H., Chen, G., Henkel-Reiger, R., Prentki, M. & Newgard, C. B. (2000)
Diabetes 49, 424 - 430.
22. Chen, G., Hohmeier, H. E., Gasa, R., Tran, V. & Newgard, C. B. (2000) Diabetes 49, 562 – 570
(from
done
INS-1, INS-2; lines 833/15 and 833/117; 832/1 INS1, 832/13 INS1, 832/2 INS1, 834/40 INS1 Cytokine Sensitive, hES2 (NIH code ES02), CyT49, GKP2 GKP4 alphaTC E14Tg2a
todo
mPAC L20 OR NHBE 6167 OR hSOX17-2 OR R7T1
National Library of Medicine - Medical Subject Headings
MeSH:Cell Line
http://www.nlm.nih.gov/cgi/mesh/2013/MB_cgimode=&term=Cell+Line&field=entry#TreeA11.251.21
0
http://stemcell.yale.edu/101/index.aspx
For more information about stem cells, see the resources below.
 NIH
The National Institutes of Health is an extensive resource on the basics of human stem cells. Information can be found at:
http://stemcells.nih.gov/info/basics/basics1.asp.
 ISSCR
A downloadable brochure on Stem Cell Facts can be obtained for free from the International Society for Stem Cell Research (ISSCR)
at: http://www.isscr.org/home/resources/learn-about-stem-cells/stem-cell-facts.
 State of Connecticut
Connecticut was fortunate to be one of the first three states in the nation to pass legislature to use public funding to support human
embryonic and adult stem cell research. More information about the Grants-in-aid program can be found at:
http://www.ct.gov/dph/cwp/view.asp?a=3142&Q=389690&dphNav_GID=1825.
http://www.isscr.org/home/resources/learn-about-stem-cells/stem-cell-faq
1. What are stem cells?
Stem cells are the foundation cells for our bodies. The highly specialized cells that make up our organs
and tissues originally came from an initial pool of stem cells that formed shortly after fertilization.
Throughout our lives, we continue to rely on persisting stem cells to repair injured tissues and replace
cells that are lost every day, such as those in our skin, hair, blood, and the lining of our gut.
8. What is a stem cell line?
Stem cells can be broadly defined by two characteristics, sometimes referred to as the “cardinal
properties of stem cells”: their capacity to self-renew (divide in a way that generates more stem cells)
and to differentiate (to turn into mature, specialized cells that make up our tissues and organs).
A stem cell line is a population of cells that can replicate themselves for long periods of time in vitro,
meaning outside of the body. These cell lines are grown in incubators with specialized growth factorcontaining media (liquid food source), at a temperature and oxygen/carbon dioxide mixture that
approximate the conditions inside the mammalian body.
stem-cell-glossary
http://www.isscr.org/home/resources/learn-about-stem-cells/stem-cell-glossary
Cell line
Cells that can be maintained and grown in culture and display an immortal or indefinite life span.
Cell type
A specific subset of cells within the body, defined by their appearance, location and function.
i) adipocyte: the functional cell type of fat, or adipose tissue, that is found throughout the
body, particularly under the skin. Adipocytes store and synthesize fat for energy, thermal
regulation and cushioning against mechanical shock
ii) cardiomyocytes: the functional muscle cell type of the heart that allows it to beat
continuously and rhythmically
iii) chondrocyte: the functional cell type that makes cartilage for joints, ear canals, trachea,
epiglottis, larynx, the discs between vertebrae and the ends of ribs
iv) fibroblast: a connective or support cell found within most tissues of the body.
Fibroblasts provide an instructive support scaffold to help the functional cell types of a
specific organ perform correctly.
v) hepatocyte: the functional cell type of the liver that makes enzymes for detoxifying
metabolic waste, destroying red blood cells and reclaiming their constituents, and the
synthesis of proteins for the blood plasma
vi) hematopoietic cell: the functional cell type that makes blood. Hematopoietic cells are
found within the bone marrow of adults. In the fetus, hematopoietic cells are found within
the liver, spleen, bone marrow and support tissues surrounding the fetus in the womb.
vii) myocyte: the functional cell type of muscles
viii) neuron: the functional cell type of the brain that is specialized in conducting impulses
ix) osteoblast: the functional cell type responsible for making bone
x) islet cell: the functional cell of the pancreas that is responsible for secreting insulin,
glucogon, gastrin and somatostatin. Together, these molecules regulate a number of
processes including carbohydrate and fat metabolism, blood glucose levels and acid
secretions into the stomach.
http://www.isscr.org/home/resources/learn-about-stem-cells/stem-cell-facts
Stem cell basics
http://stemcells.nih.gov/info/basics/pages/basics1.aspx