Microarray Sample Submission Form Project number (filled by FMSC) Project title (with one sentence) Project Description CONTACT INFORMATION Contact person(s) Name(s) Email(s) Address Phone Group leader/Principal investigator Name Email Address Phone Biocity Turku Research Programme (must be filled by Biocity Turku groups) INVOICING INFORMATION Internal invoice (University of Turku account) Name Department/Laboratory Cost pool (kustannuspaikka) TY project code: 26xxxxx External invoice (other than University of Turku account) Institution Department/Laboratory Address Cost pool (kustannuspaikka), if applicable VAT number PROJECT OPENING MEETING Date Participants (FMSC + customer) Microarray Sample Submission Form 9-2014 Page 1 of 6 MICROARRAY PLATFORM INFORMATION Array type HumanHT-12 v4 Expression BeadChip Illumina Affymetrix Agilent MouseWG-6 v2 Expression BeadChip MouseRef-8 v2 Expression BeadChip Human Genome U219 array plate Mouse MG-430 PM array plate Human/Mouse/Rat Gene 2.1 ST array plate Other, what: 8x60K array, organism: 8x15K array, organism: 4x44K array, organism: Other, what: You can find more information about the array types in our web pages (http://fmsc.btk.fi). If you have any questions about array content, please contact us. SAMPLE INFORMATION Total number of samples (including biological replicates) Organism Sample type Tissue, origin Cells, origin Blood, origin Other, what Sample material totalRNA mRNA RNA extraction method DNase treatment method (We recommend and expect that the delivered RNA samples are DNase treated. In other case contact us to discuss this) RNA is dissolved in RNAse/DNAse free water (recommended medium is Other, what: RNAse/DNAse free water, note that DEPC-water is not recommended) Other possible pre-processing done to the samples (please specify all applied protocols) Microarray Sample Submission Form 9-2014 Page 2 of 6 SAMPLE LIST Avoid spaces and any special characters in the sample names. Please use group names and sample names that will allow an easy interpretation of the name coding during data analysis. (Add rows if needed.) Also check the prices with FMSC if your total sample number has changed. Sample Sample name Sample group c, ng/l A260/A280 Volume, l Comments 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 GROUP DESCRIPTIONS Please give a short description of each sample group (add rows if needed). Please note that if you have changed your original sample setup from the one discussed with our bioinformaticians, you should contact them (bioinfo@btk.fi) to discuss the changes before continuing further. This concerns especially those projects where the data analysis service is used. Sample group Num. of biological Description replicates COMPARISON TO A SET OF PREVIOUS OR FUTURE RESULTS If you are planning to compare these results with a previous set of results produced using our service, please indicate here the time when the previous samples were processed If you are planning to compare these results directly with a possible future set of samples that will be processed using our service, please indicate this here and also give us some estimate when these samples will be processed. (It should be noted that after longer time the same equipment and protocols may not be available anymore, complicating the integration between earlier and later data set) Microarray Sample Submission Form 9-2014 Page 3 of 6 DATA ANALYSIS SERVICE I would like to purchase the full bioinformatics data analysis service (please contact the bioinformatics team for pricing and details bioinfo@btk.fi) Data will be analyzed through collaboration with the FMSC bioinformatics group (as agreed previously) I will analyze the data myself, data will be sent to this e-mail address: FILL IN DIRECT COMPARISONS BETWEEN GROUPS if FMSC's bioinformatics team will analyze the data Use group names given in the sample information table. Remember that all groups do not necessarily need to be directly compared but the lists of differentially expressed genes between several independent comparisons (e.g. for different tissues) can also be compared. Comparison Group1 0 (example) WT (wild type group) Group2 KO (knock-out group) 1 2 3 4 5 6 7 8 9 10 Paired samples Please indicate clearly (by e.g. sample naming) the samples that originate from the same biological individual as this information is needed in the analysis. Other information for the bioinformatic analysis (e.g. other factors linking certain samples together like handling day etc.) Microarray Sample Submission Form 9-2014 Page 4 of 6 SAMPLE AND DATA STORAGE LEFT OVER MATERIAL DISPOSAL FMSC will discard the left over sample material after 1 year. We prefer receiving only the amount of sample needed for the analysis but please indicate here if you for some reason need to send extra material and wish to have it returned. In such case please note that the customer has to cover the possible costs related to the returning of the sample material. STORING THE RESULT DATA Analysis results will be sent to the customer electronically and after this the customer is responsible for storing the result data. FMSC will store especially the large raw image data files only for a short time. As the extracted numerical raw data files are delivered for the customer, the original image files rarely have further use. In case you would also like to receive the raw image data files, please indicate it here. COLLABORATION WITH FMSC This page is to be filled by only those with whom collaboration has previously been agreed. Type of collaboration Lab Bioinformatics Financing of the project (What does each group contribute to material and personnel, given as detailed as possible, if relevant include payment schedule) FMSC members to be included as coauthors to publication(s) Other issues (e.g. Secrecy, place for storage of samples, time plan for discarding remains of samples, use of remaining samples for other purposes than this project) Microarray Sample Submission Form 9-2014 Page 5 of 6 Send us this sample submission form both in print with your samples and electronically as an e-mail attachment to microarrays@btk.fi. We require a signed copy of this document prior to processing the samples. Please check detailed sample submission instructions on our website at http://fmsc.btk.fi. As Principal Investigator on this project, I agree to: 1. Acknowledge the use of the Finnish Microarray and Sequencing Centre's analysis service in the materials and methods section in any resulting publications. 2. Send a reprint of each resulting publication to the Finnish Microarray and Sequencing Centre. 3. Include all involved FMSC members (see previous page) to my resulting publication(s) (where the analysis results are used) as coauthors if collaboration has been agreed with them. I will also send the manuscript for their review prior to submitting to a journal. _______________________ Date _______________________ Signature Microarray Sample Submission Form 9-2014 Page 6 of 6