OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS.CGM.SWP054
School/ Divisional Unit
School of Medical Sciences
Initial Issue date
26.6.09
Current version
1.0
Current Version
Issue date
26.6.09
Next review date
26.6.12
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Title: Use of the AGFA CP1000 Film Processor (x-ray developer)
Description: Using the X-Ray developer for illumination of antibody-HRP labeled western blots
Associated risk assessment title and location: 54_RA_Using the AGFA CP1000 Film Processor_MD
Describe the activity or process
Instructions for using the Film processor
1. All new users of the film processor must contact Angela Finch for training before using this machine. All trained users must book
the dark room before use by writing details (name of user, laboratory name and number, contact phone number) on booking
schedule (located on Rm 306 door).
2. Before beginning, check the following:
- machine is connected to power point
- the processor and dryer cover are attached and closed
- feeding and exit trays are clean
- the developer, fixer and water replenishment containers are filled and turned upside down (ensure that the taps are securely within
the machine before starting developer).
NOTE: The processor will switch off automatically if dryer assembly or processor cover is not properly closed.
3. Turn on machine by pressing the far red button (closest to the wall) and wait 10 minutes for the developer to warm up. Do not
touch other buttons.
4. Films can then be developed when the dark light is on.
5. After exposure of a film, place film quickly on the feeding tray of the developer. Once the film starts feeding the second button will
light up. Developing takes approximately 2 minutes per film with the film emerging out of the exit tray.
NOTE: Do not add another film until the second button light turns off.
Do not turn on normal lights until you have waited 20 seconds after seconds light has turned off.
6. When you have finished with the developer and all films have been removed from the exit tray, switch off the power button.
7. Turn developer (in black bag), fixer and water containers upright again. Developer reagent is light sensitive and must be kept
within a black plastic bag.
8. Sign the user log book near the processor with your details i.e. name, dept, number of films processed and note any other
comments about the developer if necessary.
Preparation of developer reagents
Developer reagents must be filled when running low.
Rinse empty containers with RO water before re-filling (located in room 307).
The reagents and their respective ingredients are listed below:
(i) Developer (labeled with a red dot)
RO water (filled up to the marked level)
Developer reagent A (add the total bottle to container)
Developer reagent B (add total bottle to container
(ii) Fixer (labeled with a blue dot)
RO water (filled up to the marked level)
Fixer solution (add total bottle to container)
(iii) Water (labeled with white dot)
RO water only (up to the mark~4L)
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
-
Latex gloves
Lab coat
Safety glasses
Dark room key
Light safe cassette
Fuji X-ray medical film
Reagents (Developer, fixer and water)
List potential hazards and risk controls including
specific precautions required
Handling reagents when re-filling containers and when handling containers. This activity could cause significant risk to the
handler
Nature of risk: Developer reagents are considered as harmful and must be handled using appropriate PPE.
* Here are the potentially hazardous chemicals found in the developing reagents:

Developer reagent A contains hydroxyquinone- irritant to skin and eyes, limited evidence of a carcinogenic effect.

Developer reagent B contains acetic acid and diethylene glycol- harmful if swallowed, irritant to skin and eyes.
Read MSDS information located on respective bottles for further information.
List emergency shutdown instructions
If film becomes jammed
If film does not emerge from the developer within 2 minutes, it may be jammed within the machine. Wait for the developer to sort out
the jam. If the jammed film does not exit the machine within 15 minutes, see the Angela Finch (ph: x51325) or Sevvandi Senadheera
(ph: x58460) for help.
If there is an electrical fault turn off machine at the power point and notify Sevvandi or Angela immediately.
List clean up and waste disposal requirements
-
Waste reagent is collected in 15 L drums located below the developing machine. These drums must be monitored to ensure
removal once full. Once a drum is full notify Angela Finch or Sevvandi for appropriate removal.
-
A biohazard waste bin is located within the dark room for all waste and must be double bagged, sealed and taken to the
nearest biohazard bin when full. Bin must also be replaced with a new bag after emptying.
-
The desk space near the processor is communal and must be kept clean and tidy at all times.
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Code of Practice for the Labelling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
Australian Standard AS2243.7-1991. Safety in laboratories. Part 7: Electrical Aspects.
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
Safe Work Procedure Form (OHS026)
Supervisory approval, training, and review
Supervisor: Peter Gunning
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licensing, training - eg course or instruction:
Training as per Training Needs Analysis, Induction into the Lab, Training in this SWP.
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Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007