The examination of biochemistry
of 86K English-class and seven-degree class. (A)
2002.07
I. Direction: Each question below contains one correct answer. Choose the most
suitable response to each question. (40 points)
1. Which one is an acidic amino acid?
A. asp B. ala C. his
D. trp
E. glu
2. Which group has the highest OD number, when the light length is 280 nm?
A. ser and thr
B. glu and lys
C. met and phe
D. asp and ser
E. trp and asp
3. Which one is the most important bond to make the secondary structure of
protein more stable?
A. Hydrophobic bond
B. Ionic bond
C. Hydrogen bond
D. Van der Waals force.
E. Disulfide bond.
4. When one molecule of pyruvate completely oxidized in mitochondria, what is
the number of substrate level phosphorylation reaction happened?
A. 1
B. 2
C. 3
D. 4
E. 5
5. The enzyme will lose its biological activity when heated, why?
A. The enzyme become amino acid
B. Damaged of primary structure
C. Damaged of secondary structure
D. Lose its solubility
E. Lose activator
6. Which reaction is irreversible in following enzyme-catalyzed reactions?
A. Aldolase
B. Pyruvate kinase
C. Phosphoglycerate kinase
D. Phosphoglycerate mutase
E. Triose phosphate isomerase
7.
Hexokinase catalyzes the first step in the glycolysis pathway. During this step,
glucose is
A. Phosphorylated to glucose-1-PO4
B. Phosphorylated to glucose-6-PO4
C. Hydrolyzed into three-carbon fragments
D. Isomerized to fructose
E. None of these
8. In general, glycolysis is inhibited by
A. ATP, glucose
B. NAD + , ATP
C. ADP, NADH
D. ATP, NADH
E. None of these
9. In gluconeogenesis (glucose synthesis), phosphoenolpyruvate is formed directly
from
A. Acetyl-CoA B. Malate C. Oxaloacetate D. Pyruvate E. None of these
10. The key enzyme of synthesis of ketone bodies is:
A. Acetyl CoA carboxylase
B. Succinatyl CoA transferase
C. HMG CoA reductase
D. HMG CoA synthetase
E. HMG CoA lyase
11. Fatty acids that must be obtained from the diet by humans include
A. Oleic acid
B. palmitoleic acid
C. palmitic acid
D. stearic acid
E. linoleic acid
12. All the following statements correctly describe ketone bodies, EXCEPT:
A. They may result from starvation
B. They are present at high levels in uncontrolled diabetes mellitus
C. They are utilized by the liver during long-term starvation
D. They include β-hydroxyl butyrate and acetone
E. They may be excreted in the urine
13. Which of the following cannot be synthesized from acetyl CoA
A. ketone bodies
B. cholesterol
C. glycerol
D.
fatty acids
E. acetone
14. Which group is consisted all by essential amino acids?
A. leu, phe, lys, ile
B.
C.
D.
E.
val, ser, thr, ile
gly, tyr, his, asp
trp, met, phe, cys
ala, lys, thr, leu
15. transamination:
A. the main way of deamination in body
B. can synthesis non-essential AAs
C. can increase the total amount of Aas in body
D. need not the cofactor
E. none of the above
16. The anti codon is located on:
A. DNA B. mRNA C. rRNA D. tRNA E. ribosome
17.Which one of the following is ketone-genesis AA?
A. ser
B. leu
C. thr
D. his
E. met
18. Which AA is the source of adrenaline?
A. ser
B. glu
C. phe
D. tyr
E. arg
19.The lipoprotein transport endogenous cholesterol is
A. CM B. VLDL C. LDL D. HDL E. IDL
20.Which of the following may be involved in the intracellular transmission of
hormone action?
A. cAMP
B. Inositol triphosphate
C. Tyrosine kinase
D. Protein kinase C
E. All of the above
21.The denaturation of DNA refers to:
A. the destroy of helix
B. the destroy of 3’-5’ phosphodiesterate bond
C. the double strand unfold into two single strands
D. the destroy of spatial structure
E. the destroy of primary structure
22.The final product of purine is
A. NH3 B. H2O,CO2
C. acetyl CoA D. uric acid E. none of the above
23. A normal and a mutant sequence on template DNA strand are shown as below.
normal …ATG CAA GTA GTC GAG CAT…
mutant …ATG CAA CTA GTC GAG CAT…
The type of mutation is
A. no phenotype change mutation
B. deletion mutation
C. insertion mutation
D. premature termination mutation
E. none of the above
24.Which one below is not a kind of direct selection method in genetic engineering:
A. antibiotic screening
B. marker rescue
C. in situ hybridization
D. nutrition rescue
E. enzyme immunodetection assay
25. The sequence acts as modification point in transcription termination in
eukaryote is:
A. UCCU B. TATAAT C. TATA D. CAAT E. AAUAAA
26. In E.coli DNA replication, the protein that recognizes the ori.C is
A. Dna A B. Dna B C. Dna C D. Dna G
E. helicase
27. Which one is the products of RNA-pol III in eukaryote?
A. snRNA B.45s-rRNA C.hnRNA D.5.8s-rRNA E. 28s-rRNA
28. In prokaryote, when transcription, which subunit in RNA-pol takes the role of
binding the DNA template?
A. α
B. σ
C. ω
D. β
E. β’
29. In eukaryotes, if a peptide that has 100 AAs will be synthesized, at least how
many molecules of ATP are needed?
A. 400 B. 399 C. 398 D. 397 E. 396
30. The enzyme which catalyzes the filling of the gap between two Okazaki
fragments during replication in prokaryote is
A. DNA ligase B. DNA gyrase C. Helicase D. DNA-pol I E. DNA-pol III
31. A codon has a base sequence, -GCC-. The complementary anticodon on the
tRNA should read:
A.-AUU- B. -CGG- C. -GGC- D. -CCGE. None of these
32. During replication, which subunit in the polymerase III recognizes the primer?
A. α B. β C. γ
D. θ
E. ε
33. The three stop codon are
A. UAA, CAA, UCC
B. UAA, UAG, UGA
C. AUG, AGU, AUU
D. UCC, UCA, UAC
E. UCG, UGC, UCC
34. Who use experiment to determine the semi-conservative replication of DNA
firstly?
A. Watson & Crick
B. Kornberg
C. Messelson & Stahl
D. Nierenberg
E. Jacob & Monod
35. A certain kind of restriction endonuclease can recognize 6 base pairs, it will cut
a DNA strand like this: 5’-AGCTG AATTC-3’(one strand only), what kind of
end will be made?
A. 5’ sticky end
B. 3’ sticky end
C. both 5’ and 3’ sticky end
D. either 5’ or 3’ sticky end
E. blunt end
36. In the SOS response, which protein acts as an inhibitor
A. Lex A
B. Rec A
C. Uvr A
D. Lex B
E. Rec B
37. In the tryptophan operon the attenuation activity takes place when
A. sequence 1 binds with sequence 2 to form a hairpin structure
B. sequence 1 binds with sequence 3 to form a hairpin structure
C. sequence 2 binds with sequence 3 to form a hairpin structure
D. sequence 2 binds with sequence 4 to form a hairpin structure
E. sequence 3 binds with sequence 4 to form a hairpin structure
38. A signal peptide of polypeptide chain is required for which of the following
reaction
A. the synthesis of internal proteins
B. the synthesis of polyribosomal units
C. the attachment of ribosome to mRNA
D. the attachment of ribosome to endoplasmic reticulum
E. the release of peptides from ribosomes
39. Which of the following actions below must involve the activity of virus
A. conjugation
B. transformation
C. transduction
D. transposition
E. recombination
40. The S-D sequence is on the
A. 3’ end of 16s-rRNA
B. 5’ end of mRNA
C. 3’ end of 23s-rRNA
D. 3’ end of mRNA
E. 5’ end of 16s-rRNA
II. Fill up the blanks. (10 points. 0.5 point each)
1. The sources of heme are__________, _________, ___________.
2. The specificity of enzyme-catalyzed reactions includes ___________,
_______________, and _______________.
3. Hormones raising blood sugar level are _____ and________.
4. The four steps of fatty acid β-oxidatio are ____________, ______________,
_____________, and ________________.
5. The sources of one carbon unit are ______________, _________,
_____________, and _____________.
6. In eukaryote, when DNA replication, the enzyme that catalyzes the formation
of leading strand is ______, the lagging strand is _____; the enzyme that acts in
mitochondria is ________; the enzyme that takes the role of proofread is
________.
III. Explain the following concepts. (10 points, 2 points each)
1.
2.
3.
4.
5.
motif
trans-acting factor
cori cycle
secondary messenger
de novo synthesis of nucleotide
IV. Answer the following questions. (20 points, 4 points each)
1.
2.
3.
4.
5.
Please describe the NADH respiratory chain.
Please describe the sources and outlets of NH3.
Please describe the system of protein biosynthesis (translation).
Please describe the mechanism of lac operon regulation.
Please describe the types and functions of membrane receptor.
V. 实验题(10 分, 每空 0.5 分)
1
PCR 反应的成分包括______, ________,__________,_______,______.
2
电泳系统的不连续性是指_______,___________,__________.
3
支持物对电泳结果的影响因素有_________，______，_________。
4
检查 NH4 的存在可用_________。
5
蛋白质的检测用__________。
6
EDTA 可以防止酶解，是因为_______________________。
7
DNA 提取实验中，测定 DNA 纯度是通过测定_________，然后计算，纯 DNA 该值
＋
=________.
8
DNA 分子在 PH7.0 溶液中带______电荷,是由于 DNA 分子中______基团的解离引
起的.
9
DNA 电泳的支持物为__________，电泳方式_________。
10 测定蛋白质的方法是________，_______，___________。