Liang et al. – Supplementary Table – 1
Supplementary Table 1 Strains and plasmids used in this study
Strains or plasmid
Genotype or relevant characteristics
Source or reference
Escherichia coli
supE44, ΔlacU169(Φ80lacZΔM15), hsdR17,
DH5α
recA1, gyrA96, thi-1, relA1
Agrobacterium
a Kmr derivative of EHA101, Rifr
Hood et al. (1990)
Vector containing the P1 phage Cre site-specific
Sauer and Henderson (1990)
Invitrogen
tumefaciens EHA105
pBS185
recombinase gene, Ampr
pGEM-T
Cloning vector for PCR products, Amp r
Promega
pGEM-TCre
P1 phage Cre site-specific recombinase
This study
PCR-amplified from pBS185 and cloned into
pGEM-T , Ampr
pRT104
Vector containing the CaMV 35S promoter and a
Töpfer et al. (1987)
poly-(A) signal, Ampr
pBluescript II SK
High copy number ColE1-based phagemid, Ampr
Stratagene
pBluescript-loxP
pBluescript II SK derivative containing two loxP
This study
sites
pBI101
Cloning vector containing the β-glucuronidase
Jefferson et al. (1987)
(Gus) gene of E. coli and the nopaline synthase
gene terminator (Tnos) from pBIN19, Kmr
pBluescript-loxP-Tno
pBluescript II SK derivative containing a 2 kb
s-loxP-Gus
loxP-Tnos-loxP-Gus cassette
pGreen0000
Binary vector for Agrobacterium-mediated plant
This study
Hellens et al. (2000)
Liang et al. – Supplementary Table – 2
transformation, requires the pSoup helper plasmid
for replication in A. tumefaciens, Kmr
pSoup
Helper plasmid required for replication of
Hellens et al. (2000)
pGreen0000 in A. tumefaciens , Kmr
pGreen-loxP
pGreen0000 derivative carrying a 2.7 kb
This study
35S-loxP-Tnos-loxP-Gus cassette (Fig. 1B), Kmr
pGreen-Cre
pGreen0000 derivative carrying a 1.5-kb 35S-Cre
This study
sequence (Fig.1A), Kmr
pCambia2301
Binary vector for Agrobacterium-mediated plant
http://www.cambia.org
transformation, Kmr
pCambia-loxP
pCambia2301 derivative carrying a 2.7-kb
This study
35S-loxP-Tnos-loxP-Gus cassette (Fig. 1B), Kmr
pCambia-Cre
pCambia2301 derivative carrying a 1.5-kb
This study
35S-Cre sequence (Fig. 1A), Kmr
pPZP112
Binary vector for Agrobacterium-mediated plant
Hajdukiewicz et al. (1994)
transformation, Cmr
pPZP-loxP
pPZP112 derivative carrying a 2.7-kb
This study
35S-loxP-Tnos-loxP-Gus cassette (Fig. 1B), Cmr
pISV2678
Binary vector for Agrobacterium-mediated plant
M. Schultze and
transformation; derivative of pGPTV-BAR
E. Kondorosi CNRS,
(Becker et al. 1992) with a double CaMV 35S
Gif-sur-Yvette, France
promoter and a translational enhancer sequence
from pBI-426 (Datla et al. 1991), Kmr
pISV-Cre
pISV2678 derivative carrying a 1.5-kb 35S-Cre
This study
sequence (Fig.1A), Kmr
Rifr, Ampr, Kmr, Cmr, resistance to rifampin, ampicillin, kanamycin and chloramphenicol, respectively.
Liang et al. – Supplementary Table – 3
References
Becker D, Kemper E, Schell J, Masterson R (1992) New plant binary vectors with selectable markers
located proximal to the left T-DNA border. Plant Mol Biol 20:1195-1197
Datla RS, Hammerlindl JK, Pelcher LE, Crosby WL, Selvaraj G (1991) A bifunctional fusion between
beta-glucuronidase and neomycin phosphotransferase: a broad-spectrum marker enzyme for
plants. Gene 101:239-246
Hajdukiewicz P, Svab Z, Maliga P (1994) The small, versatile pPZP family of Agrobacterium binary
vectors for plant transformation. Plant Mol Biol 25:989-994
Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible
binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819-832
Hood EE, Clapham DH, Ekberg I, Johannson T (1990) T-DNA presence and opine production in
tumors of Picea abies (L.) Karst induced by Agrobacterium tumefaciens A281. Plant Mol Biol
14:111-117
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and
versatile gene fusion marker in higher plants. EMBO J 6:3901-3907
Sauer B, Henderson N (1990) Targeted insertion of exogenous DNA into the eukaryotic genome by the
Cre recombinase. New Biol 2:441-449
Töpfer R, Matzeit V, Gronenborn B, Schell J, Steinbiss HH (1987) A set of plant expression vectors for
transcriptional and translational fusions. Nucleic Acids Res 15:5890