Banque de Tissus pour la Recherche
Laboratory Procedures for Human Tissues
August 2004
_______________________________________________________________
NITROGEN
FREEZING PROTOCOL FOR MUSCLE BIOPSY
Equipment and Materials
Cryotubes (2 ml) with screw-top and silicon joints.
Liquid nitrogen.
 Dry ice
 "Transport form-human tissue specimens" 1 containing the information necessary
for optimal utilization of tissues (this information will be rendered anonymous ; the
donor cannot be identified).


Procedure
1. Place the specimen in a dry tube.
2. Close the tube and label it (please note that certain types of ink are erased in liquid
nitrogen).
3. Plunge the tube in liquid nitrogen.
4. Fill out the specimen fact sheet if necessary.
5. Store the tube in a freezer at -80°C or in dry ice until sending it, or collection by our
services.
1
The specimen fact sheet is provided by the bank
BTR – Cécile Jaeger - August 2004
Copyright Eurobiobank 2005
1/1