The Mutational Pattern of Primary Lymphoma of the Central Nervous System
Determined by Whole Exome Sequencing
Running Title: Mutational Pattern of PCNSL
Inga Vater*1, Manuel Montesinos-Rongen*2, Matthias Schlesner*3, Andrea Haake1,
Frauke Purschke2, Rosanne Sprute2, Nina Mettenmeyer2, Ilias Nazzal2, Inga Nagel1,
Jana Gutwein1, Julia Richter1, Ivo Buchhalter3, Robert B. Russell4,5, Otmar D.
Wiestler6, Roland Eils3,5,7, Martina Deckert**2, Reiner Siebert**1
1Institute
of Human Genetics, Christian-Albrechts-University Kiel & University
Hospital Schleswig-Holstein, Campus Kiel, Kiel Germany
2Institute
3Division
of Neuropathology, University of Cologne, Cologne, Germany
of Theoretical Bioinformatics (B080), German Cancer Research Center
(DKFZ), Heidelberg, Germany
4Cell
Networks, University of Heidelberg, Heidelberg, Germany
5BioQuant,
6German
Heidelberg University, Heidelberg, Germany
Cancer Research Center (DKFZ), Heidelberg, Germany
7Department
for Bioinformatics and Functional Genomics, Institute for Pharmacy and
Molecular Biotechnology (IPMB), Heidelberg University, Heidelberg, Germany
*These authors contributed equally to this work
** These authors contributed equally to this work
Tables
Supplementary Table 1. Primers
Supplementary Table 2. Sequence variants (after filtering)
Supplementary Table 3. Genes recurrently affected by protein changing variants
Figure Legends
Supplementary Figure 1. Verification of the Whole Exome Sequencing Method
Supplementary Figure 2. Expression of the recurrently mutated genes mining on
gene expression profiling of PCNSL
Supervised analysis of PCNSL vs. DLBCL for all mutated genes, which affected at
least two PCNSL, significantly differentially expressed tags were calculated based on
Student's t-test. Only tags that were at least 2-fold significantly differentially
expressed are shown. Samples of normal brain tissue were included for comparison.
2
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