DNA Sequencing Core University of Michigan Affymetrix Full Service Sample Submission Form: Genotyping Date: Principal Investigator: ____________________ Sequencing Core PI login:____________________ E-mail:______________@_________________ Lab Contact:____________________________ E-mail:______________@_________________ Phone:____________________________________ Service* I. a. b. Full Service processing of Total RNA 15ul x 50ng/ul in Low EDTA TE – SNP6 (1) 20ul x 10ng/ul in Low EDTA TE – Axiom (1) a. b. c. d. Affymetrix Arrays Human Genome SNP 6.0 (1 array) Axiom Biobank Arrays (96 arrays)† Other: _______________________ User Supplied:_________________ II. Cost Quantity Subtotal $334.00 $ 33.00 _______ _______ _______ _______ $ 300.00 $ 9600.00 $ $N/A Total _______ _______ _______ _______ _______ _______ _______ _______ *Please complete both sections as best as possible. †Please note that Axiom submissions must be in an AB-gene 96 Square Well Storage Plate (Catalogue Number: AB-0932) from Thermo Scientific. Transferring samples to the correct plate is available for a fee. More arrays are available than listed above; please consult the Affymetrix website (http://www.affymetrix.com) and/or the Microarray Core website (http://www.umich.edu/~caparray/products/genotyping/). By signing below I agree to the following terms: 1. 2. 3. Agree that the items and services request above will be recharged upon completion of project. Any rebilled arrays and reagents have a 6-month shelf-life from time of receipt at the Core. The core is not responsible for projects not completed within 6 months of initial request. Project Name:______________________________________________________________________ Billing Department____________________________________ UM Short Code:|__|__|__|__|__|__| Print Principal Investigator Name: ______________________________________________________ Principal Investigator Signature:_____________________________ Date:____________________ 2/16/2016 University of Michigan MicroArray Core Total DNA Sample Submission Form Please attach a plate map (see sample below) to this document and a list of samples submitted as an Excel spreadsheet via email. Thank you. Well 1 2 3 4 5 6 7 8 9 10 A B C D E F G H DLA0927 DLA0929 DLA0930 DLA0935 DLA0941 DLA0945 DLA0949 DLA0951 DLA0953 DLA0957 DLA0958 DLA0959 DLA0963 DLA0966 DLA0968 DLA0969 DLA0974 DLA0976 DLA0977 DLA0979 DLA0981 DLA0984 DLA0986 DLA0989 DLA0990 DLA0992 DLA0995 DLA0996 DLA1001 DLA1003 DLA1007 DLA1008 DLA1012 DLA1014 DLA1016 DLA1022 DLA1023 DLA1026 DLA1029 DLA1030 DLA1031 DLA1032 DLA1035 DLA1042 DLA1046 DLA1048 DLA1052 DLA1054 DLA1056 DLA1057 DLA1059 DLA1060 DLA1062 DLA1063 DLA1065 DLA1068 DLA1070 DLA1072 DLA1075 DLA1076 DLA1078 DLA1082 DLA1088 DLA1089 DLA1091 DLA1092 DLA1095 DLA1097 DLA1098 DLA1101 DLA1104 DLA1105 DLA1188 DLA1190 DLA1195 DLA1196 DLA1197 DLA1198 DLA1106 DLA1107 11 Control 01 DLA1199 DLA1200 DLA1201 DLA1202 DLA1205 DLA1109 DLA1113 12 Control 02 DLA1207 DLA1208 DLA1209 DLA1211 DLA1212 DLA1115 DLA1120 Data Analysis: Please provide or attach a description of the experimental data analysis you would like performed when the arrays are completed. Example: Samples 1-3 (Control untreated), 4-6 (PC3 untreated), 7-9 (Control drug treated 3hr), and 10-12 (PC3 drug treated 3hr) are biological replicates. I am interested in comparing the gene expression of the control vs PC3 cells, the treated vs untreated, and the interaction between cell type and treatment status. 2/16/2016