DNA Sequencing Core University of Michigan Affymetrix Full Service Sample Submission Form: Gene Chip/Atlas Date: Principal Investigator: ____________________ Sequencing Core PI login:____________________ E-mail:______________@_________________ Lab Contact:____________________________ E-mail:______________@_________________ Phone:________________________________ ___ Service* I. Cost Quantity Subtotal a. b. Full Service processing of Total RNA Std: min. of 250ng and 50ng/ul NuGen: min. of 10ng and 2ng/ul $180.00 $212.00 _______ _______ _______ _______ a. b. c. d. e. f. g. Affymetrix Arrays Human Gene ST 2.1 Strip (4 arrays) Human U219 Strip (4 arrays) Human U133 Plus 2.0 (1 array) Mouse Gene ST 2.1 Strip (4 arrays) Mouse 430 2.0 Strip (4 array) Other: _______________________ User Supplied:_________________ $500.00 $320.00 $250.00 $500.00 $1000.00 $ N/A _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ II. Total _______ *Please complete both sections as best as possible. More arrays are available than listed above; please consult the Affymetrix website (http://www.affymetrix.com) and/or the Microarray Core website (http://www.umich.edu/~caparray/gene_expression/). By signing below I agree to the following terms: 1. 2. 3. Agree that the items and services request above will be recharged upon completion of project. Any rebilled arrays and reagents have a 6-month shelf-life from time of receipt at the Core. The core is not responsible for projects not completed within 6 months of initial request. Project Name:______________________________________________________________________ Billing Department____________________________________ UM Short Code:|__|__|__|__|__|__| Print Principal Investigator Name: ______________________________________________________ Principal Investigator Signature:_____________________________ Date:______________________ 2/12/2016 University of Michigan MicroArray Core Total RNA Sample Submission Form* P.I. Name & Sample # Concentration (NanoDrop/UV) Volume Submitted (minimum of 10 uL) Example: Krebs_001 125 ng/uL 10 uL 1.______________________ _____________________ ____________________ 2.______________________ _____________________ ____________________ 3.______________________ _____________________ ____________________ 4.______________________ _____________________ ____________________ 5.______________________ _____________________ ____________________ 6.______________________ _____________________ ____________________ 7.______________________ _____________________ ____________________ 8.______________________ _____________________ ____________________ 9.______________________ _____________________ ____________________ 10._____________________ _____________________ ____________________ 11._____________________ _____________________ ____________________ 12._____________________ _____________________ ____________________ *Please see our website for sample naming convention (http://www.umich.edu/~caparray/sample_policy/). When the project is completed, please contact us to arrange sample collection. Data Analysis: Please provide or attach a description of the experimental data analysis you would like performed when the arrays are completed. Example: Samples 1-3 (Control untreated), 4-6 (PC3 untreated), 7-9 (Control drug treated 3hr), and 10-12 (PC3 drug treated 3hr) are biological replicates. I am interested in comparing the gene expression of the control vs PC3 cells, the treated vs untreated, and the interaction between cell type and treatment status. 2/12/2016