DNA Sequencing Core University of Michigan Affymetrix Full Service Sample Submission Form: Date: Principal Investigator: ____________________ Sequencing Core PI login:____________________ E-mail:______________@_________________ Phone:____________________________________ Lab Contact:_____________________________ E-mail:______________@_________________ Service I. a. b. c. d. e. f. g. h. II. Affymetrix GeneChips Human Gene ST 2.1 Strip (4 arrays) Human U219 Strip (4 arrays) Human U133 Plus 2.0 (1 array) Mouse Gene ST 2.1 Strip (4 arrays) Mouse 430 2.0 (1 array) Rat Gene ST 2.1 Strip (4 arrays) Other: _______________________ User Supplied:_________________ Full Service processing of Total RNA a. Std: >250ng and >50ng/ul b. NuGen: >10ng and >2ng/ul Total Sequencing Core PI login:____________________ Phone:____________________________________ Cost Quantity Subtotal $500.00 $320.00 $250.00 $500.00 $250.00 $500.00 $ $N/C _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ _______ $220.00 $310.00 _______ _______ _______ _______ _______ By signing below I agree to the following terms: 1. 2. 3. Agree that the items and services request above will be recharged upon completion of project. Any rebilled GeneChips and reagents have a 6-month shelf-life from time of receipt at the Core. The core is not responsible for projects not completed within 6 months of initial request. Membership support: __Cancer Center __Shock Center __Rheumatic Diseases ___MDRTC __GI/Peptide Center Project Name:______________________________________________________________________ Billing Department____________________________________ UM Short Code:|__|__|__|__|__|__| Print Principal Investigator Name: ______________________________________________________ Principal Investigator Signature:_____________________________ Date:______________________ University of Michigan Affymetrix GeneChip® Array Core Total RNA Sample Submission Form Name/Sample # NanoDrop/UV Conc. Volume Submitted 1.______________________ _____________________ ____________________ 2.______________________ _____________________ ____________________ 3.______________________ _____________________ ____________________ 4.______________________ _____________________ ____________________ 5.______________________ _____________________ ____________________ 6.______________________ _____________________ ____________________ 7.______________________ _____________________ ____________________ 8.______________________ _____________________ ____________________ 9.______________________ _____________________ ____________________ 10._____________________ _____________________ ____________________ 11._____________________ _____________________ ____________________ 12._____________________ _____________________ *** Please pick up your samples ASAP once your experiment is completed. ____________________ Data Analysis: Please provide or attach a description of the experimental data analysis you would like performed when the arrays are completed. Example: Samples 1-3 (Control untreated), 4-6 (PC3 untreated), 7-9 (Control drug treated 3hr), and 10-12 (PC3 drug treated 3hr) are biological replicates. am interested in comparing the gene expression of the control vs PC3 cells, the treated vs untreated, and the interaction between cell type and treatment status.