Developmental Biology Course Curie Institute- UPMC
In collaboration with the Developmental and Regenerative Biology graduate program
of HARVARD University
Intensive laboratory and lecture course for M2 and PhD students: 5 week-long
including 3 weeks of practicals and 2 weeks of lectures (limited to 18 students)
Topics of lectures: Worm model in developmental biology, Evolution and Development,
Early vertebrate development, , Drosophila as a genetic model, Signalling pathways
during development, Plant development, Mesoderm derivatives, Stem cells during
development, Development of the nervous system, Development of marine organisms
Practicals: genetic model system (C. elegans, Drosophila, zebrafish, mouse) and wellestablished experimental attributes (frog, chick).
September 26- October 15: PRACTICAL PART
C. Elegans practical: S. Louvet and V. Galy (UPMC, Paris)
Realisation and observation of mutants, olfactive tests
Zebrafish practical: E. Hirsinger, E. Havis, F. Giudiceli (Pasteur Institute, UPMC,
Paris)
Ablation of the shield. Analysis of Cyp26a1 as a target of the Iroquois gene. Injection
of Cyp26a1-GFP and analysis of operated embryos.
Xenopus practical: M. Umbhauer, JF. Riou, C. Carron, O. Bronchain (Genopole,
UPMC, Paris)
Manipulating embryonic axes, Spemann experiment, mesoderm induction in vitro,
Nieuwkoop recombination, gastrulation in vitro.
Mouse practical : E. Buffin, JP. Chambon (UPMC, Paris): Culture of mouse oocytes
and early mouse embryos
Avian practical: C. Drevon, C. Fournier-Thibault, T. Jaffredo, S. Escot (UPMC,
Paris)
Whole embryo culture. Intracardiac retroviral injection. Chick-quail grafts. Neural tube
electroporation. Neural crest cell culture
Drosophila practical : A. Audibert, M. Gho, E. Buffin (UPMC, Paris)
Transgenic embryo analysis : study of cuticule, wing and leg imaginal discs, formation of
mecano-sensorial organs.
Mouse practical :
R. Griffone, S. Cerreghini, (UPMC, Paris)
Myotome study in mouse: study of the Six mouse. Development of organs derived from
the primitive intestine : study of the vHNF1 mouse.
C. Fournier-Thibault, S. Escot, (UPMC, Paris)
Mouse Embryo and Organ Culture
October, 18- 28: LECTURES
Curie Institute, Development and Genetics department :
Edith Heard (Curie Institute, Paris)
Guillaume Balavoine (IJM, Paris)
P. Lemaire (IBDML, Marseille)
Ascidian embryos: from the birth of experimental embryology to the analysis of gene
regulatory networks.
Nicolas David (ENS, Paris)
Molecular control of endoderm development and differentiation in zebrafish
Philippe Herbomel (Pasteur Institute, Paris)
The zebrafish reveals the odyssey of hematopoietic precursors in developing
vertebrate embryos
Ian Drummond (Harvard University, Cambridge, USA)
Laurent Kodjabachian (IBDM, Marseille)
Motile Ciliogenesis in Vertebrate
Enrique Amaya (Manchester University, GB)
Roberto Mayor (UCL, London)
Collective cell migration. The case of the neural crest
Thomas Lecuit (IBDM, Marseille)
Closing in on mechanisms of tissue morphogenesis.
Julius Brennecke (Vienna)
François Schweisguth (Pasteur Institute, Paris)
Asymetric cell division in Drosophila
Yohanns Bellaiches (Curie Institute, Paris)
Regulation of epithelial cell polarity during development
Daniel St Johnston (Cambridge, UK)
Jan Traas (Ecole Normale Supérieure, Lyon)
From genes to shape at the shoot meristem
Deborah Bourc’his (Curie Institute, Paris)
Epigenetic decisions and reproduction in mammals.
Michel Labouesse (IGBMC, Strasbourg)
Genetic analysis of epithelial morphogenesis in C. elegans
Thomas Holstein (University of Heidelberg)
Stem cells in cnidarian regeneration
Cliff Tabin (Harvard University, Boston)
New insights into vertebrate limb development
O.Pourquié (IGBMC, Strasbourg)
Developemental Patterning of the vertebrate embryonnic axis
Claudio Stern (University College of London, Londres)
Gastrulation through a primitive streak: cellular mechanics and signals
Claudio Stern (University College of London, Londres)
Building a brain: a molecular dissection of neural induction
Fillipo Del Bene (Curie Institute, Paris)
Paul Trainor (Stowers Institute, Kansas City)
Neural Crest Cells: Evolution, Development and Disease
Jean-René Huynh (Curie Institute)
Germline stem cells in Drosophila
Charles Ffrench-Constant (University of Edinburgh)
The microenvironment of the embryonic neural stem cell: lessons from adult niches.
Anne Grappin (Swiss institute of Cancer research, Lausanne)
Pancreas development, stem cells and cancer
Thierry Jaffredo (UPMC, Paris)
Frédéric Relaix (Myology Institute, UPMC, Paris)
Skeletal muscle stem and progenitor cells during development.