The manuscript title: Evolutionary and Functional Novelty of Pancreatic
Ribonuclease: a Study of Musteloidea (order Carnivora)
Author list: Jiang Liu, Xiao-ping Wang, Soochin Cho, Burton K. Lim, David M.
Irwin, Oliver A. Ryder, Ya-ping Zhang, Li Yu
Supplementary Information
Supplementary Data 1. Isoelectric points (pIs) of RNASE1s by species. pIs were
calculated by Isoelectric Point Calculator (http://isoelectric.ovh.org)
Supplementary Data 2. Isoelectric points (pIs) of RNASE1s by gene groups. pIs were
calculated by Isoelectric Point Calculator (http://isoelectric.ovh.org)
Supplementary Data 3. (a) Species tree of Caniformia, including families and
subfamilies examined in this study. Because there is no consensus about the
phylogenetic relationships among the subfamilies and species in Mustelidae, so we
presented that part as a polytomy in the tree, while those among the families in
Caniformia, which are generally recognized, are presented as dichotomy in the tree. (b)
Phylogenetic tree of all 94 RNASE1 sequences. The phylogenetic tree was generated
from NJ analysis. Pseudogenes are marked by Ψ. Gene duplication events are
indicated.
Supplementary Data 4. The mapping of the positive selected residues identified in
the present study on the 3-dimensional crystal structure of the molecule, using Pymol
Ver 0.99 based on the crystal structure of the bovine Rnase1 (PDB accession 1C0B).
The active sites were indicated by red, and the positive selected sites were indicated
by green. The numbers near the arrows represent the amino acid positions in bovine
Rnase1, and the numbers within the parenthesis represent the corresponding positions
in the present study.
Supplementary Data 1.
Supplementary Data 2.
Supplementary Data 3.
Supplementary Data 4
.