Biology II – Chapter 7 Self Test 1. In most land plants, photosynthesis occurs in cells of the ___ of the leaves, because these cells contain the largest numbers of chloroplasts. cuticle Epidermis Stomata vascular bundles Mesophyll 2. Light energy is initially captured by "photosystems" within thylakoid membranes. Photosystems are organized arrays of _____. pigment molecules such as carotenoids chlorophyll molecules proteins all of the above none of the above 3. Why is carbon dioxide a key molecule in the light-independent reactions of photosynthesis? Carbon dioxide is the major product of the light-independent reactions of photosynthesis. Carbon dioxide provides electrons to replace those lost by chlorophyll during the light-dependent reactions. Carbon dioxide inhibits the light-independent reactions of photosynthesis. Carbon dioxide, together with water, is the raw material for the synthesis of sugars, which are the key products of the light-independent reactions. 4. What is photorespiration? Photorespiration is the process by which plants produce energy at night. Photorespiration is the process by which plant cells cool off in hot climates. Photorespiration is the process that prevents sugar production in C3 plants when CO2 levels are low and O2 levels are high. Photorespiration is the process by which plants capture light energy and convert it into ATP. 5. In the light-dependent reactions of photosynthesis, ATP is produced by chemiosmosis. Describe this process. When light strikes the chlorophyll molecules, water is moved via osmosis across the chloroplast membrane. When water moves back across the membrane, ATP is generated. Chemiosmosis is the process by which water moves across a semipermeable membrane. As high-energy electrons move from carrier to carrier in the electron transport system of the thylakoid membrane, some of the energy is captured to pump hydrogen ions across it. 6. A lovely tree called the flowering plum has beautiful pink flowers in spring and deep-purple leaves in summer. What types of pigments are plentiful in this plant? Can photosynthesis occur in these purple leaves? Explain your answer. The leaves probably contain a high amount of chlorophyll and carotenoids and lesser amounts of phycocyanins than do plants with green leaves. The leaves probably contain a high amount of carotenoids and lesser amounts of phycocyanins and chlorophyll than do plants with green leaves. The leaves probably contain a high amount of phycocyanins and carotenoids and less chlorophyll than do plants with green leaves. They can still perform photosynthesis, because photosynthesis can occur to some extent at all wavelengths of light. 7. Take a deep breath and slowly exhale. The oxygen in that breath is being used by your mitochondria in reactions that produce ATP from sugars and other food molecules you ate. Where did that oxygen come from originally? The oxygen in the atmosphere is produced by the breakdown of carbon dioxide during photosynthesis. The oxygen in the air is produced by the splitting of water during the light-dependent reactions of photosynthesis. Plants produce the oxygen via the process of photorespiration, in which they break down sugars at night when photosynthesis cannot occur. Plants produce the oxygen via the process of photorespiration when they break down sugars in their mitochondria. 8. A scientist studying photosynthesis illuminated a culture of algae with bright visible light. She then turned out the light and simultaneously began to bubble radioactive CO2 gas into the culture. After 30 minutes, she stopped the reaction and measured the amount of radioactivity inside the cells. What did she find? Explain your answer. There was radioactivity in the cells, because the CO2 is used to synthesize sugar, even in the dark. There was no radioactivity inside the cells, because the CO2 is used to produce O2 in the light-dependent reactions. Thus, there was radioactivity in the air above the culture but not in the cells. There was no radioactivity in the cells, because light is required to produce sugars from CO 2 and water. There was radioactivity inside the cells, because CO2 is used to replace the electrons that were lost by chlorophyll when the lights were turned on. 9. Imagine that you are trying to set up a large fish tank and want to select a colored light that will show off the fish to best advantage but will also allow the green plants in the tank to grow and stay healthy. You decide to measure the efficiency of photosynthesis by looking at the production of oxygen bubbles on the leaves (see Figure 7-5). At first, you use a white fluorescent lamp and see many oxygen bubbles on the leaves, indicating that photosynthesis is occurring normally. Next, you put a sheet of green cellophane between the white light and the water so that the light coming through to the tank appears green. Will the oxygen bubbles remain the same, increase, decrease, or disappear? Explain your answer. The bubbles will disappear, because photosynthesis cannot occur in green light. The bubbles will remain constant because photosynthesis can occur to some extent at all visible wavelengths of light. The bubbles will increase; because chlorophyll is green, photosynthesis will work best if leaves are exposed to green light. The bubbles will decrease, because green light cannot be absorbed by chlorophyll. 10. Which of the following is a true statement about photosynthesis? In photosynthesis, inorganic molecules such as carbon dioxide and water react to produce organic, energyrich molecules such as glucose. Photosynthesis is a process that is carried out primarily by autotrophic prokaryotic bacteria. In photosynthesis, oxygen is used to help break down glucose. Photosynthesis is an exergonic reaction. 11. Leaves include a number of structural modifications for the purpose of photosynthesis, including __________. adjustable openings in the surface of the leaf that permit the passage of carbon dioxide, water, and oxygen a waxy covering on the surface of the leaf that is designed to reduce evaporation photosynthetic mitochondria Both the first and second answers are correct. 12. During photosynthesis, electrons are continuously lost from the reaction center of photosystem II. What source is used to replace these electrons? sunlight water carbon dioxide oxygen 13. The term carbon fixation refers to __________. the loss of carbon during glucose synthesis the incorporation of atmospheric carbon dioxide into a larger organic molecule the regeneration of ribulose bisphosphate the synthesis of glyceraldehyde-3-phosphate 14. Photorespiration occurs when __________. oxygen is combined with ribulose bisphosphate carbon dioxide is combined with ribulose bisphosphate stomata are closed Both the first and third answers are correct. 15. How have C4 plants adapted to environmental conditions that would result in increased photorespiration? PEP specifically combines with carbon dioxide even in the face of high oxygen concentrations. C4 plants have substituted phosphoenolpyruvate (PEP) for ribulose bisphosphate. A molecule transports the fixed carbon into a cell type where the normal C3 synthetic metabolism would be favored due to the now high concentrations of carbon dioxide. all of the above 16. The stomata __________. allow oxygen to exit allow carbon dioxide to enter allow light to enter allow excess heat to exit The first two answers are correct. 17. You have just discovered a new plant with red-orange leaves. What wavelengths of visible light are NOT being absorbed by this pigment? green, blue, and violet green green and yellow red and orange blue and violet 18. In the light-dependent reactions of photosynthesis the difference in hydrogen ion concentration across the thylakoid membrane is used to generate __________. glucose oxygen FADH2 NADPH ATP 19. Which of the following represents the products of the light-dependent reactions of photosynthesis? For what process are these products needed? NADPH and glucose; light-independent reactions carbon dioxide and water; Calvin-Benson cycle ATP and oxygen; photosystem I NADPH and ATP; Calvin-Benson cycle NADH and ADP; cellular respiration 20. Which of the following is NOT required for the light-independent reactions of photosynthesis? CO2 NADPH O2 stroma H2O ATP 21. _____________________________ converts the energy of sunlight into chemical energy. 22. ____________________________ are organisms that can produce their own food in their cells. 23. Photosynthesis takes place within ____________________________. 24. In the _________________________________, light energy is converted to chemical energy in the form of ATP and NADPH. 25. In the ___________________________________, chemical energy is used to drive the formation of glucose. 26. _____________________________ is the key light-capturing molecule found in chloroplasts. 27. Complete the following cycle of reactions. For each item below, select the letter that labels the correct part of the image. ________ - light-dependent reactions (thylakoids) ________ - light-independent reactions (stroma) ________ - ATP, NADPH ________ - ADP, NADP+ 28. Using the image below, provide the connections between photosynthesis and cellular respiration. For each item below, select the letter that labels the correct part of the image. ________ - sugar ________ - O2 (oxygen) ________ - ATP (adenosine triphosphate) ________ - H2O (water) ________ - CO2 (carbon dioxide) 29. Label the structures in this cross section of a leaf. For each item below, select the letter that labels the correct part of the image. ________ - mesophyll cells ________ - stoma ________ - vascular bundle ________ - cuticle ________ - upper epidermis ________ - chloroplasts 30. Below is a diagram of light-dependent reactions of photosynthesis. Place the descriptions of the steps in this process next to the appropriate portion of the diagram. For each item below, select the letter that labels the correct part of the image. ________ - Electrons are passed to the electron transport system. ________ - Light is absorbed by the light-harvesting complex of photosystem II and energy is passed to the reaction center chlorophyll molecule. ________ - Electrons are ejected out of the energy center. ________ - Due to the action of the electron transport system, a hydrogen gradient is created in thylakoids, generating ATP. ________ - The electrons from photosystem I are captured in an electron transport system and used to create NADPH. ________ - Light strikes photosystem I, causing it to emit electrons. 31. Photosynthesis is measured in the leaf of a green plant exposed to different wavelengths of light. Photosynthesis is highest in green light highest in red light highest in blue light highest in red and blue light the same at all wavelengths 32. Where do the light-dependent reactions of photosynthesis occur? in leaf cell mitochondria within the thylakoid membranes of the chloroplast in the stomata in the leaf cell cytoplasm in the chloroplast stroma 33. The oxygen produced during photosynthesis comes from the breakdown of CO2 the breakdown of oxaloacetate the breakdown of both CO2 and H2O the breakdown of H2O photorespiration 34. The role of accessory pigments is to allow photosynthesis to occur in the dark prevent photorespiration donate electrons to chlorophyll reaction centers provide an additional photosystem to generate more ATP capture additional light energy and transfer it to the chlorophyll reaction centers 35. The generation of ATP by electron transport in photosynthesis and cellular respiration depends on a proton gradient across a membrane proton pumps driven by electron transport chains an ATP-synthesizing enzyme complex 1, 2, and 3 are all required for ATP generation none of the above are required for ATP generation 36. Where do the light-independent, carbon-fixing reactions occur? in the chloroplast stroma at night in the thylakoids in the guard cell cytoplasm within the thylakoid membranes in mitochondria KEY 1. In most land plants, photosynthesis occurs in cells of the __________ of the leaves, because these cells contain the largest numbers of chloroplasts. mesophyll 2. Light energy is initially captured by "photosystems" within thylakoid membranes. Photosystems are organized arrays of __________. all of the above 3. Why is carbon dioxide a key molecule in the light-independent reactions of photosynthesis? Carbon dioxide, together with water, is the raw material for the synthesis of sugars, which are the key products of the lightindependent reactions. 4. What is photorespiration? Photorespiration is the process that prevents sugar production in C3 plants when CO2 levels are low and O2 levels are high. 5. In the light-dependent reactions of photosynthesis, ATP is produced by chemiosmosis. Describe this process. As high-energy electrons move from carrier to carrier in the electron transport system of the thylakoid membrane, some of the energy is captured to pump hydrogen ions across it. 6. A lovely tree called the flowering plum has beautiful pink flowers in spring and deep-purple leaves in summer. What types of pigments are plentiful in this plant? Can photosynthesis occur in these purple leaves? Explain your answer. The leaves probably contain a high amount of phycocyanins and carotenoids and less chlorophyll than do plants with green leaves. They can still perform photosynthesis, because photosynthesis can occur to some extent at all wavelengths of light. 7. Take a deep breath and slowly exhale. The oxygen in that breath is being used by your mitochondria in reactions that produce ATP from sugars and other food molecules you ate. Where did that oxygen come from originally? The oxygen in the air is produced by the splitting of water during the light-dependent reactions of photosynthesis. 8. A scientist studying photosynthesis illuminated a culture of algae with bright visible light. She then turned out the light and simultaneously began to bubble radioactive CO2 gas into the culture. After 30 minutes, she stopped the reaction and measured the amount of radioactivity inside the cells. What did she find? Explain your answer. There was radioactivity in the cells, because the CO2 is used to synthesize sugar, even in the dark. 9. Imagine that you are trying to set up a large fish tank and want to select a colored light that will show off the fish to best advantage but will also allow the green plants in the tank to grow and stay healthy. You decide to measure the efficiency of photosynthesis by looking at the production of oxygen bubbles on the leaves (see Figure 7-5). At first, you use a white fluorescent lamp and see many oxygen bubbles on the leaves, indicating that photosynthesis is occurring normally. Next, you put a sheet of green cellophane between the white light and the water so that the light coming through to the tank appears green. Will the oxygen bubbles remain the same, increase, decrease, or disappear? Explain your answer. The bubbles will decrease, because green light cannot be absorbed by chlorophyll. 10. Which of the following is a true statement about photosynthesis? In photosynthesis, inorganic molecules such as carbon dioxide and water react to produce organic, energy-rich molecules such as glucose. 11. Leaves include a number of structural modifications for the purpose of photosynthesis, including __________. Both the first and second answers are correct. 12. During photosynthesis, electrons are continuously lost from the reaction center of photosystem II. What source is used to replace these electrons? water 13. The term carbon fixation refers to __________. the incorporation of atmospheric carbon dioxide into a larger organic molecule 14. Photorespiration occurs when __________. Both the first and third answers are correct. 15. How have C4 plants adapted to environmental conditions that would result in increased photorespiration? all of the above 16. The stomata __________. The first two answers are correct. 17. You have just discovered a new plant with red-orange leaves. What wavelengths of visible light are NOT being absorbed by this pigment? red and orange 18. In the light-dependent reactions of photosynthesis the difference in hydrogen ion concentration across the thylakoid membrane is used to generate __________. ATP 19. Which of the following represents the products of the light-dependent reactions of photosynthesis? For what process are these products needed? NADPH and ATP; Calvin-Benson cycle 20. Which of the following is NOT required for the light-independent reactions of photosynthesis? O2 21. __________ converts the energy of sunlight into chemical energy. photosynthesis 22. __________ are organisms that can produce their own food in their cells. autotrophs 23. Photosynthesis takes place within __________. chloroplasts 24. In the __________, light energy is converted to chemical energy in the form of ATP and NADPH. light-dependent reactions 25. In the __________, chemical energy is used to drive the formation of glucose. light-independent reactions 26. __________ is the key light-capturing molecule found in chloroplasts. Chlorophyll 27. 28. Complete the following cycle of reactions. light-dependent reactions (thylakoids) A. light-independent reactions (stroma) B. ATP, NADPH C. ADP, NADP+ D. Using the image below, provide the connections between photosynthesis and cellular respiration. sugar D. O2 (oxygen) E. ATP (adenosine triphosphate) C. H2O (water) A. CO2 (carbon dioxide) B. 29. 30. Label the structures in this cross section of a leaf. mesophyll cells C. stoma F. vascular bundle E. cuticle A. upper epidermis B. chloroplasts D. Below is a diagram of light-dependent reactions of photosynthesis. Place the descriptions of the steps in this process next to the appropriate portion of the diagram. Electrons are passed to the electron transport system. C. Light is absorbed by the light-harvesting complex of photosystem II and energy is passed to the reaction center chlorophyll molecule. A. Electrons are ejected out of the energy center. B. Due to the action of the electron transport system, a hydrogen gradient is created in thylakoids, generating ATP. D. The electrons from photosystem I are captured in an electron transport system and used to create NADPH. F. Light strikes photosystem I, causing it to emit electrons. 31. E. Photosynthesis is measured in the leaf of a green plant exposed to different wavelengths of light. Photosynthesis is highest in red and blue light 32. Where do the light-dependent reactions of photosynthesis occur? within the thylakoid membranes of the chloroplast 33. The oxygen produced during photosynthesis comes from the breakdown of H2O 34. The role of accessory pigments is to capture additional light energy and transfer it to the chlorophyll reaction centers 35. The generation of ATP by electron transport in photosynthesis and cellular respiration depends on 1, 2, and 3 are all required for ATP generation 36. Where do the light-independent, carbon-fixing reactions occur? in the chloroplast stroma