NAYUN KIM, PH.D.
324 Aaron Circle
Durham, NC 27713
nk43@notes.duke.edu
(919) 684 - 0042
PROFILE OVERVIEW
Doctoral research molecular biologist with practical, laboratory and
management experience in the areas of molecular genetics, molecular
immunology, and microbiology with research focus on the DNA repair pathways
and genomic instability.
EDUCATION
August 2000 Ph.D. Biochemistry and Molecular Biology
Thesis title “Transcription and DNA repair in immunoglobulin gene
somatic hypermutations”
Department of Biochemistry and Molecular Biology, University of
Chicago, Chicago IL
June 1994
B.S. Chemistry
University of Chicago, Chicago IL
WORK EXPERIENCE
Sept 2006 to present
Research Associate Senior
Duke University Medical Center
Department of Molecular Genetics and Microbiology
My advisor Dr. Sue Jinks-Robertson has relocated to Duke
University Medical Center in Sept 2006. After helping with
the move and set-up of the new lab at the new location, I am
continuing with my research in genomic instability in
Saccharomyces cerevisiae.
Sept 2003~Sept 2006
Postdoctoral Fellow
Emory University
Department of Biology. Atlanta, GA
I am taking a molecular genetic approach to study
transcription-associated DNA mutagenesis and
recombination, using the model system Saccharomyces
cerevisiae. I am also studying the molecular basis of “AtRisk” sequence motifs implicated in tumorigenesis using a
yeast model system.
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Advisor: Professor Sue Jinks-Robertson, Ph.D.
Sept 2000 – Aug 2003
Postdoctoral Fellow
University of Pittsburgh
Department of Pharmacology Pittsburgh, PA
I created recombinant expression and purification protocols
for a novel human DNA polymerase using human tumor cell
line HELA cells, Sf9 insect cell system, and E. coli bacterial
cell system.
Advisor: Professor Richard D. Wood, Ph.D.,F.R.S.
1994 – 2000
Ph.D. Candidate
University of Chicago
Dept. of Biochem. and Mol. Bio. Chicago, IL
My graduate research focused on the molecular process
involved in the affinity maturation and repertoire
diversification of activated B lymphocytes.
Advisor: Professor Ursula Storb, M.D
MOLECULAR, BIOCHEMICAL, AND CELL BIOLOGICAL TECHNIQUES
RNA, DNA manipulation, PCR, RT-PCR, Real-time Quantitative
RT-PCR, electrophoresis, Southern, Northern and Western
analysis, Fluorescent Activated Cell Sorting analysis, DNA
polymerase activity assay using radio-labeled nucleic acid, protein
Affinity purification, Fluorescent microscopy, Ion exchange Fast
Protein Liquid Chromatography (FPLC), human and murine primary
cell culturing, transgenic mice, di-deoxy terminating Sequencing,
and SSCP (single strand conformation polymorphism) analysis
using radio-labeled nucleic acid.
TEACHING EXPERIENCE
Fall 2004 & 2005
Emory University Atlanta, GA
Teaching Assistant/ Biology 141
Instructor for the laboratory exercises in introductory cell and
molecular genetics course.
Fall 1999
University of Chicago Chicago, IL
Teaching Assistant/ BioSci 181
Assistant for the introductory cell and molecular biology
course for advanced undergraduate science major students.
Fall 1995
University of Chicago Chicago, IL
Teaching Assistant/ BMB 311
Protein Structure and Function – Graduate course in the
introduction to the biochemical study of protein.
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PUBLICATIONS
Kim, N. and Jinks-Robertson, S. 2009 “dUTP incorporation into genomic DNA is
linked to transcription in yeast.” Nature 459:1150 – 1153.
Kim, N., Abdulovic, A., Gealy, R., Lippert, M., and Jinks-Robertson, S. 2007
“Transcription-associated mutagenesis in yeast is directly proportional to the
level of gene expression and influenced by the direction of DNA replication” DNA
repair 6:1285 – 1296.
Abdulovic, A., Kim, N., and Jinks-Robertson, S. 2006 “ Mutagenesis and the
three R’s in yeast” DNA repair 5:409 – 21.
Marini, F., Kim, N., Schuffert, A., and Wood, R. 2003 “POLN, a nuclear POLA
family DNA polymerase homologous to the DNA cross-link sensitivity protein
Mus308” The journal of biological chemistry 278:32014 – 32019.
Michael, N., Shen, H. M., Longerich, S., Longacre, A., Kim, N., and Storb, U.
2003 “’The E-box motif, CAGGTG, is an enhancer of somatic hypermutation
without enhancing transcription.” Immunity 19:235 – 242.
Kim, N., Martin, T. E., Simon, M. C., and Storb, U. 2003 "The transcription
factor Spi-B is not required for somatic hypermutation" Molecular Immunology
39:577 – 583.
Michael, N., Martin, T. E., Nicolae, D., Kim, N., Padjen, K., Zhan, P., Nguyen, H.,
Pinkert, C., and Storb, U. 2002 “Effects of sequence and structure on the
hypermutability of immunoglobulin genes.” Immunity 16:123 – 134.
Shen, H. M., Michael, N.,Kim, N., and Storb, U. 2000 “The TATA binding
protein, c-MYC and survivin genes are not somatically hypermutated, while Ig
and BCL6 genes are hypermutated in human memory B cells.” International
Immunology 12:1085 – 1093.
Storb, U., Shen, H. M., Michael, N., and Kim, N. 2000 "Somatic hypermutation
of immunoglobulin and non-immunoglobulin genes" Philosophical Transactions
of the Royal Society 356:13 – 20.
Storb, U., Peters, A., Kim, N., Shen, H.M., Bozek, G., Michael, N., Hackett, Jr,
J., Klotz, E., Reynolds, J.D., Loeb, L.A., and Martin, T.E. 1999 “Molecular
aspects of somatic hypermutation of immunoglobulin genes.” Cold Spring
Harbor Symposia on Quantitative Biology 64:227 – 34.
Kim, N., Bozek, G., Lo, J. C., Storb, U. 1999 “Different mismatch repair
deficiencies all have the same effects on somatic hypermutation: intact primary
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mechanism accompanied by secondary modifications.” Journal of Experimental
Medicine 190:21-30.
Kim, N., and Storb, U. 1998 “The role of DNA repair in somatic hypermutation of
immunoglobulin genes.” Journal of Experimental Medicine 187:1729-1733.
Storb, U., Peters, A., Klotz, E., Kim, N., Shen, H. M., Kage, K., Rogerson, B.,
Martin, T. E. 1998 “Somatic hypermutation of immunoglobulin genes is linked to
transcription.” Current Topics in Microbiology and Immunology 229:11-18
Storb, U., Peters, A., Klotz, E., Kim, N., Shen, H. M., Rogerson, B., and Martin,
T. E. 1998 “Cis-acting sequences that affect somatic hypermutation of Ig
genes.” Immunological Reviews 162:153-160.
Kim, N., Kage, K., Matsuda, F., Lefranc, M.-P., and Storb, U. 1997 “B
lymphocytes of xeroderma pigmentosum or Cockayne syndrome patients with
inherited defects in nucleotide excision repair are fully capable of somatic
hypermutation of immunoglobulin genes.” Journal of Experimental Medicine
186:413-419.
Zent, C., Kim, N., Hiebert, s., Zhang, D. E., Tenen, D. G., Rowley, J. D., and
Nucifora, G. 1996 “Rearrangement of the AML1/CBFA2 gene in Myeloid
leukemia with the 3;21 translocation : Expression of co-existing multiple chimeric
genes with similar functions as transcriptional repressors, but with opposite
tumorigenic properties.” Current Topics in Microbiology and
Immunology 211:243-252.
ABSTRACTS and PRESENTATIONS
“The role of replication orientation and endogenous DNA damage in
Transcription-associated mutagenesis in Saccharomyces cerevisiae” Gordon
Research Conference on Mutagenesis 2006
“Probing Transcription stimulated mutagenesis (TAM) in Saccharomyces
cerevisiae using Tetracycline-regulative Dual Expression System” Gordon
Research Conference on Mammalian DNA Repair 2005
“Somatic Hypermutation of Ig genes and DNA mismatch repair” Keystone
Symposium on B cell immunobiology and disease 1999
“Somatic Hypermutation of Ig genes and transcription” Keystone Symposium on
Transcription regulation 1997
HONORS, AWARDS
1998 and 1999
Committee on Cancer Biology Travel Grant
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1998
June 1994
June 1994
REFERENCES
Howard Tager Memorial Travel Grant
General Honors at Graduation (B.S.)
Induction to Phi Beta Kappa
1. Professor Sue Jinks-Robertson, Ph.D.
Duke University Medical Center
Department of Molecular Genetics and Microbiology
228 Jones Building, Research Drive Box3020
Durham, NC 27710
Phone: (919) 681 – 7273, Fax: (919) 684 – 2790
sue.robertson@duke.edu
2. Professor Richard D. Wood, Ph.D.
University of Pittsburgh
Pittsburgh Cancer Institute
5117 Centre Avenue, Research Pavilion, Suite 2.6
Pittsburgh, PA 15213
Phone: (412) 623 – 7762, Fax: (412) 623 – 7761
rdwood@pitt.edu
3. Professor Ursula Storb, M.D.
University of Chicago
Department of Molecular Genetics and Cell Biology
920 E. 58th Street CLSC1061
Chicago, IL 60637
Phone: (773) 702 – 4440
stor@midway.uchicago.edu
4. Professor Gray Crouse, Ph.D.
Emory University Department of Biology
1009 Rollins Research Center
1510 Clifton Road
Atlanta, GA 30322
Phone: (404) 727 – 4236, Fax: (404) 727 – 2880
gcrouse@biology.emory.edu
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