Cell Biology Membrane Transport Note: please remember that these are abbreviated lecture notes and that I often cover additional material in class. Also, the notes provided are not meant to replace your reading the text. Membrane Transport - Regulation of traffic of nutrients, wastes, ions - Membranes are selectively permeable o Sugars, amino acids, nucleotides, ions Too polar/charged to pass Require transport proteins o O2, CO2 and H2O Freely pass Types of Transport - Simple Diffusion - Passive Transport (facilitated diffusion) o Requires transport protein - Active Transport o Requires transport protein + energy Simple Diffusion - high concentration to low concentration - no energy required - small molecules; O2, CO2 and H2O Osmosis - movement of H2O across a membrane from a region of low to high [solute] - hypotonic solution – lower solute conc. than inside - hypertonic solution – higher solute conc. than inside - isotonic solution – same Osmoregulation (control of water balance) - most animals cells live in a hypotonic solution 2 o intracellular solutes e.g. negatively charged ions/organic molecules - water tends to flow in; can burst cell - Animal cells o Continuously pump out inorganic ions (e.g. Na+) o Primary purpose of Na+/K+ pump - Plants (and algae, fungi, bacteria) o cell wall keeps from bursting o cells become turgid (firm) o turgor pressure forces out as much water that comes in o in hypertonic solution, plants wilt (plasmolysis) Transfer of Uncharged Solutes - determined only by concentration gradient - by diffusion or transport proteins - e.g. glucose Cellular Ion Concentrations - [ion] in cytosol differs from extracellular fluid - cytosol is more negative charged (more anions) - maintained by ion specific transport mechanisms Inside Cell Outside Cell Ca2+ Na2+ K+ Membrane Potential - difference in electrical charge (voltage) across membrane - cytoplasmic side is more negative - favors passage of cations into cell; anions out of cell Transfer of Charged Solutes 2 3 - electrochemical gradient o concentration gradient + membrane potential o can work in same direction Na+ o Can work in opposite direction; little movement K+ Passive Transport - movement of substance down its concentration or electrochemical gradient - requires ion channel or carrier protein Passive transport via Ion channels - Multipass membrane proteins - Hydrophilic pore - Selective o Na+, K+, Cl-, Ca2+ o Size and charge - Direction determined by electrochemical gradient - Fast (1000x faster than transport by carrier proteins) - Open or closed depending on conformation o Ligand-gated o Stress-activated (mech. Force) auditory hair cells o Voltage-gated – nerve and muscle cells; venus fly trap Passive Transport via Carrier Proteins - Multipass transmembrane proteins - Selective – ions, sugars, amino acids, nucleotides - Binding triggers conformational change - Direction according to conc. or electrochemical gradient 3 4 - Types o Uniports – 1 molecule in one direction Passive glucose carriers Determined by conc. gradient o Symports – 2 different molecules in same direction o Antiports – 2 different molecules in opposite direction Active Transport - movement of substance against its concentration or electrochemical gradient - requires carrier protein - requires energy input o ATP hydrolysis o Existing ion gradient Na+-K+ pump (Na+-K+ ATPases) - Animal cells - Antiport carrier protein - Pumps out 3 Na+; pumps in 2 K+ - Uses ATP - Uses approximately 1/3 of energy in most cells! - Function o Maintains osmotic balance; keeps cells from bursting o Extracellular Na+ gradient drives in other molecules Active Transport of Glucose into Animal cells - high glucose in cytosol; low glucose in extracellular fluid - Na+-K+ pump creates high extracellular [Na+] - Glucose driven in via glucose-Na+ symport 4 5 Active Transport of Sucrose into Plant Cells - plants and bacteria do not have Na+-K+ pumps - use H+-ATPases (H+-pumps) o H+ gradient used to drive in sucrose via sucrose-H+ symport H+-ATPases - also used to regulate pH in animal and plant cells - e.g. lysosomes 5