Prelab Questions
DNA Extraction
Name: _________________________
Biotechniques Virtual Lab: http://learn.genetics.utah.edu/units/biotech/extraction/
1. What are some reasons to isolate DNA from humans?
2. List some of the necessary tools/chemicals for extracting DNA:
3. How does proteinase K help unravel the chromosomes into strands of DNA?
4. How do you balance a centrifuge if you have an odd number of tubes? Why is this
important?
5. After centrifuging the solution the first time, where is the DNA? Where is the unwanted
cell debris?
6. When the DNA is placed in isopropyl alcohol (ethanol), it comes out of solution. Based
on your knowledge of DNA, is ethanol a polar or nonpolar substance?
7. After centrifuging the solution a second time, where is the DNA?