Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
No
1-
Item
Quantity Specifications
Preparative HPLC
modular, stackable, and comprised of a gradient pumping system,
Autosampler, UV/visible spectrophotometric detector, fraction
collector and software control and data processing package.
- Origin for this equipment (all parts, accessories, spare parts
etc): USA, Europe, Japan
- Operating manuals and maintenance manual included.
- Training period required
Item
Solvent delivery system:
High pressure gradient
formation by the
combination of two isocratic
pump modules with
appropriate connecting
tubing and software control
so that the units work
together as one gradient
solvent delivery system.
Quantity specifications
Each isocratic module shall
1
have:
- Dual pistons in parallel
- Flow Range setpoints from 0
-
upto 100 mL/min, in 0.001
mL/min increments.
Operating Pressure range from 2
to 40 MPa (20–400 bar, 290-5880
psi).
Flow precision: <0.3 % RSD in
range from 1 mL/min to 100
mL/min
Pump Delay volume: 700 μL or
less
- Tool kit
- Software controlled automated
electromagnetic purge valve.
- Automated seal wash for
-
-
Autosampler
1
-
extended seal lifetime. Safety
features: Leak detection and safe
leak handling (shuts system down
if leak occurs), error detection
and status display.
Communications: Controller-area
network (CAN) connection to
other modules, RS232C, remote
start/stop/prepare connection.
Storage of electronic records of
maintenance, usage and errors
with notification when pre-set
usage limits are reached to allow
maintenance planning.
Must be equipped with dualinjection-loop flow path for
analytical and preparative scale
injections without changing the
loop.
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 1
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
- Must be installed with both 50
-
-
μL and 5000 μL (5mL)
injection loops.
Injection modes: Both Partial
loop filling and Complete loop
filling available on both loops.
Injection range: 0.1 - 5000 μL
in 0.1 μL increments.
Area precision for Complete
loop fill:
Typically < 0.5% RSD (50 μL
loop, 3x overfill).
Sample viscosity range: 0.2 - 5
cp
Carryover: < 0.1% with rinse
factor > 3
Ability to automatically rinse or
flush the inside and outside of the
injection needle with wash
solvent for further reduction of
carryover.
Draw/eject rate of metering unit
up to 50 ml/min (selectable in 1
μl/min steps)
Must be upgradeable to
thermostatted operation by
simple addition of an optional
Peltier temperature control
module allowing temperature
control of the samples from 4 to
40 °C.
Sample capacity, the following
formats to be available:
- 2 well-plates (96 and 384) plus 10
additional 2-mL vials;
or - 108 x 2-mL vials in 2 × 54 vial plate
plus 10 additional 2-mL vials;
or - 30 x 6-mL vials in 2 × 15 vial plate
plus 10 additional 2-mL vials;
or - 54 Eppendorf tubes (0.5/1.5/2.0 mL)
in 2 × 27 Eppendorf tube plate
Safety features:
- Leak detection and safe leak
handling (shuts system down
if leak occurs), error detection
and status display.
Communications:
- Controller-area network
(CAN) connection to other
modules, RS232C, remote
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 2
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
Detector 1:
1
Multi-wavelength UV/visible
detector
start/stop/prepare connection.
Storage of electronic records of
maintenance, usage and errors with
notification when pre-set usage
limits are reached to allow
maintenance planning.
- Multi-wavelength UV/visible
detector with minimum of 5
independent signals
- Wavelength range: 190 to 950
nm
- Signal bandwidth: variable from
2nm to 400nm
- Reference wavelengths and
bandwidths can be set on all
signals
- Light source: Both Deuterium
and Tungsten lamps
- Detection type: photodiode array
with resolution smaller than 1nm
per diode
- Wavelength accuracy: ± 1 nm
- Verification of wavelength
accuracy with built-in holmium
oxide filter.
- Short term noise (ASTM) Single
and Multi-Wavelength: < ± 0.7 x
10-5 AU at 254 and 750 nm
- Drift: < 0.9 x 10-3 AU/h at 254
nm
- Linear absorbance range: > 2 AU
(5 %) at 265 nm
- Optical Slit width: Programmable
with minimum 1nm, standard
4nm and maximum 16 nm
resolution settings
- Supplied with preparative 3mm
path length flow cell
- Supplied with preparative 0.3mm
path length flow cell
- standard 10mm path length flow
cell
- Safety features: Leak detection
and safe leak handling (shuts
system down if leak occurs),
error detection and status display
- Communications: Controller-area
network (CAN) connection to
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
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Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
-
Detector 2: Refractive
index
1
-
-
other modules, RS232C, remote
start/stop/prepare connection
Storage of electronic records of
maintenance, usage and errors
with notification when pre-set
usage limits are reached to allow
maintenance planning.
Liquid contact material: stainless
steel, PTFE and quartz glass.
Range: 1.00 – approximately 1.8
RIU, calibrated
Measurement range: +/- 600 ×
106 RIU
Temperature for optics: from
ambient to 55 °C.
Sample cell: 8 μL, maximum
pressure: 5 bar(0.5 Mpa),
maxiumum flow rate: 5mL/min.
Valves: automatic purge and
automatic solvent recycle.
pH range: Approximate range 2 –
98 or better.
Automatic zero before analysis
Volume: Inlet port to sample cell
62 μL, inlet port to outer port
590 μL
Time programmable parameters:
polarity, peak width.
Performance: short time noise <
+/- 2.5 × 10-9 RIU, drift lower
than 200 × 10-9 RIU/h
Optical zeroing: through set
screw
Control and data evaluation: PC
control with suitable software for
control and data evaluation.
Parameter entry, signal display,
on-line help and diagnostic
should be through control
module. Optional card for
method for method sequence and
logbook storage and transfer.
Max. Data rate: up to 37 Hz.
Safety and maintenance:
Extensive diagnostics, error
detection and display, leak
detection, safe leak handling, leak
output signal and shutdown of
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
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Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
pumping system.
- Communications: Controller-area
Fraction Collector
1
network (CAN) connection to
other modules, RS232C, remote
start/stop/prepare connection.
- Storage of electronic records of
maintenance, usage and errors
with notification when pre-set
usage limits are reached to allow
maintenance planning.
- Delay calibration sensor: Single
wavelength absorbance detector
working at 654 nm, consisting of
a LED and a photo diode, can be
automatically positioned at end of
fraction delivery needle to detect
blue dye calibrant peak. Allows
easy calibration of detector to
fraction collector delay volume
for accurate collection of
fractions.
- Flow rate capability up to
100mL/min
- Allows pooling of fractions from
multiple injections.
- Able to use well plates,
Eppendorf tubes, and vials and
test tubes of different heights and
widths for fraction collection.
- Fraction containers:
96 deep-well plates up to 48 mm
height;
Test tubes, minimum 48 mm up to
maximum 100 mm height (up to
45 mL); Autosampler vials (2 mL
and 6 mL)
Fraction collection full-size trays:
 4 well plates
 40 × 45 mL test tubes (30 mm
OD, 100 mm height)
 60 × 32 mL test tubes (25 mm
OD, 100 mm height)
 126 × 13 mL test tubes (16 mm
OD, 100 mm height)
 215 × 7.5 mL test tubes (12 mm
OD, 100 mm height)
 2 well plates + 10 x 2 mL vials (+
one possible additional half tray)
 100 × 2 mL (+ one possible
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 5
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
additional half tray)
 Tray for 40 collecting funnels
- Forced fume extraction to allow
-
-
-
-
-
Control and Data
Processing Software
1
-
the use of autosampler outside
fume cipboard.
Fraction Collection Trigger
modes: Time slices, Peak
triggered from detector
(threshold, up- / downslope),
Timetable (combination of time
intervals and peak)
Must be upgradeable to
thermostatted operation by
simple addition of an optional
Peltier temperature control
module allowing temperature
control of the fractions from 4 to
40 °C.
Safety features: Leak detection
and safe leak handling (shuts
system down if leak occurs),
error detection and status display
Communications: Controller-area
network (CAN) connection to
other modules, RS232C, remote
start/stop/prepare connection.
Storage of electronic records of
maintenance, usage and errors
with notification when pre-set
usage limits are reached to allow
maintenance planning.
Software controls all functions of
the LC modules
- Software
shows status of
instrument
modules
and
parameters on a graphical user
interface
- Allows methods to be stored
containing
the
instrument
parameters and data processing
parameters
- Automated sequences can be
setup to process multiple samples
and to turn off the pump and
detector at the end of the
sequence.
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 6
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
- System
offers
standard
qualitative
and
quantitative
reports and customised reports
- Reports include fraction reports
showing how the fractions were
acquired and which containers
they were stored into.
- Graphical setting of threshold,
up-/downslope, upper threshold
values on example chromatogram
for ease of determining values.
Required additional items for the prep-HPLC:
Tool kit with screwdrivers
1
and wrenches required for
routine maintenance of
system
Analytical scale C-18 column
Prep Scale column
Prep Scale C18 column, 50
mm i.d.
Vials
1
Test tubes and trays for
fraction collector
1
ITEM
2-
1
1
UV-Vis double beam
Spectrophotometer
1
Analytical scale C-18 column
column C-18 column, 21.2 or 30
mm i.d.
C-18 column, (50 mm i.d).
for use with auto-sampler, both
2mL and 6mL, and appropriate
trays to hold them
Quantity. Specifications
1
- 1nm Resolution or better, satisfying
the standards of wavelength
resolution demanded by the European
Pharmacopoeia.
- Fully compliance with FDA 21 CFR
Part 11.
- Has a built in USB port for direct
connection with printer, data copy on
a USB memory stick.
- Including software for data analysis
Photometric analysis, spectrum mode,
quantitation mode, kinetics mode,
time scan, multi component
quantitation mode.
- Including laser printer, Personal
computer, LCD display screen with
software for analysis and data
processing.
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 7
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
- LCD display screen on the instrument
showing spectrums for easy access
for students
- Including a pair of UV Cell Quartz.
 Optical system: Double beam
 Wave length range: 190 – 1100 nm
 Spectral band width: 2 nm (190 –
1100 nm) or better
 Wavelength setting : 0.1 nm
increments
 Wave length accuracy: ±0.3 nm
 Wave Length reproducibility: ≤ 0.2
nm
 Speed Scan: 3000 to 2 nm/min or
better
 Photometric Mode: Transmittance,
Absorption, Concentration
 Wavelength slew rate: about 6000
nm/min
 Photometric range: - 4.0 – 4.0 Abs,
transmittance:
0%
up
to
approximately 400 %
 Photometric accuracy: ± 0.002 A
(0.0-0.5 A), ± 0.004 (0.5 – 1.0A), ±
0.006 Abs (2.0 Abs) and/or 0.3% T
(0-100%T) or better.
 Stray light: ≤ 0.02% T (220 nm NaI,
340 nm NaNO2), less than 1.0%
KCl (198 nm).
 Baseline stability: less than
0.0003Abs/hr (700 nm, 0Abs), after
2 hours of turning lamp on or use
(or warm-up).
 Baseline flatness: about or within ±
0.0006 Abs (190-1100nm, one hour
after light source turned on)
 Noise level: within 0.00005 Abs
(700 nm) 30 min warm-up.
 Light source: Deuterium D2 and
Tungsten halogen lamps, Built in
light source and auto position
adjustment
 Monochromator:
Blazed
holographic grating in CzernyTuner mounting
 Detector: Silicon photodiode.
 Connections and parallel port.
 Cell holder: 4 cell.
 Power supply: Switchable 120-
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 8
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
230VAC, 50-60 Hz.
 PC (Brand name).
 Training included.
 Operation and service manuals are
included.
 Certificate of conformity.
 Origin (for the instrument and all
needed accessories: Europe, USA
or Japan).
3-
Water jet vacuum
pump
1
4-
Refrigerated bath
circulator
(Recirculating cooler)
1
5-
water-jet aspirator
4
 Ultimate vacuum: 23 hPa (mbar) at
20°C and 7 hPa (mbar) at 1°C
circulating water temperature
 Suction capacity: 4.500 L/h
 Supply: 230V/50Hz, 520W, singlephase
Origin (for the pump and all needed
accessories: Europe, USA or Japan)
 Capacity: 8-14 L
 Cooling capacity: 1200W at 10ºC
 Temperature range: -20 - + 30 °C
 Control accuracy: ±2ºC or better.
 Pump rate: 18-20 L/min
 Display: LED four digits display
 Material: - Bath: Stainless steel; Case:
powder coated
 Safety: over current and leakage
breaker.
 Approximate dimensions: overall (w×
d×h, mm): 400 ×600 × 650
 Electrical supply: 230 V, 50-60 Hz
 Temperature controller: Digital PID
controller
 Digitally set temperature
 Quiet operation
Origin (for the instrument and all
needed accessories: Europe, USA or
Japan).
 Portable dual-channel,
 Bath volume (L/cu ft) 9.5/0.3
 Max. vacuum capacity (Mpa/Hg):
0.0998/29.5
 Free air capacity: 18 L/minute (0.6
cfm × 2ea)
 Material: Path: polypropylene,
Aspirator: Nickle coated brass X 2ea
 Dimensions: (bath (W × L, D)
(mm/in) 298 × 210/11.7 × 8.3, 8.9
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 9
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
 Overall (W×L×H) (mm/in): 330×
265× 390/ 13.0 × 10.4× 15.4
 Inhale nozzle: internal diameter 9.5
mm (0.4) × 2ea
 Drain cook, over flow nozzle
(mm/inch): Ø 17.0 /0.7
 Electrical requirements: 230 V, 50/60
Hz, 0.7 A
 Accessories: cooling coil, Aspirator
Origin (for the instrument and all
needed accessories: Europe, USA or
Japan, Korea).
6-
Electrical Heating mantel
suitable for 2L Round
Bottom flasks.
2
7-
Digital Abee
Refractometer
4
8-
Analytical Balance
3
 Maximum temperature: 450 °C
 Indicator lamps for power and heater
operation.
 A chemically resistant outer case with
polypropylene or epoxy coating.
 Use with round bottom flasks 2L.
 Built in energy regulator.suppleid
with clamps for 1.3 cm support tods.
 Certificate required.
 Nickel connectors.
 Thermally insulated case, mineral
wool fiber insulation.
 Safety earth connection
 Dimensions (d x w x h), mm: 400 x
350 x 190
 230V 50/ 60Hz, 500W, EU Plug
 Weight: Maximum 3 kg.
- Origin (for for the heating
mantel and all needed
accessories: Europe, USA or
Japan).
 Refractive index: 1.3000-1.7000 (±
0.0002); Brix Bx-TC: 0-95%, (± 0.1
%); Brix Bx: 0-95% (± 0.1 %),
Temperature working range 0 -50 °C,
 - training included.
Origin: Europe, USA or Japan.
 Fully automatic balance, 4 digits
 Made of chemically resistant
material
 With glass shield side and top
 Max capacity: 200 – 210 g
 Readability: 0.0001g
 Repeatability: ±0.0001g (0.1mg)
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 10
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
 Pan size: Ø range 80-90 mm
 Linearity: Linearity: ± 0.0002
 Large easy to read high contrast
LCD
 Sealed keypad
 Stainless Steel weighing pan
 Internal, manual calibration
 Automatic zero adjustment
 Units of measurements: g, ct and oz.
 Tare range by subtraction up to
maximum capacity.
 Level adjustment (bubble).
 Operable temperature range
ambient 5 °C to 40 °C.
 Automatic calibration with external
weight modes, Dust cover.
 Stability time 10 seconds or better
 Power supply 220-240 V
 With English service and instruction
manuals.
Origin: USA, EU or Japan
9-
Electronic precision
balance
4
10-
Digital Avometer
30
11- Analogue Melting point
4
apparatus + thermometer






Capacity: up to 410 g
Readability: 0.001g
Repeatability: ±0.001g
Linearity: ±0.002
Pan size: Ø approximately 120 mm
Large easy to read high contrast
LCD
 Sealed keypad
 Stainless Steel weighing pan.
 Protective in use cover.
 Multiple weight modes.
 Internal, manual calibration
 Dust cover
 Automatic calibration with external
weight modes, Dust cover.
 Power Supply: 230 Hz, 50-60 Hz
Origin: Europe, USA or Japan.
 (digital).
 Origin: Europe, USA or Japan
Melting point Apparatus, complete with
thermometer and pack of 100 melting
point tubes (open both ends).
 Number of samples: up to 3
 Temperature range: ambient to
300°C
 Heating rate: 1 to 10°C/min
 Net weight: should not exceed
2 kg.
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 11
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories




12- Conductivity meters
4
(bench top)
Electrical supply: 230 V, 50
Hz, 50 W.
Easy to operate.
Spare thermometer (2 for each
apparatus)
Origin: Europe, USA or
Japan.
- Bench top with large screen
- Measures conductivity and TDS
- Both automatic and manual setting
temperature
- Compensation with fixed coefficient,
(2 % or better).
- Calibration with standards of
conductivity and TDS.
- Temperature measuring range: 0-60
C.
- RS-232 output to connect to a printer
or computer
- Temperature sensor connection Rt2252.
- Conductivity ranges: 0-20, 200, 200
μS/cm; 0-10 mS/cm. precision: 1.5 %
or better.
- TDS range: 0-1000 mg/L
- Operating manual + maintenance
manual.
- Origin: USA, Europe, Japan
13
Integrated GC-MS
System
1
Fully operable of an integrated Gas
Chromatograph/Mass
Spectrometer
(GC/MS) system of high performance
with consistent ultra-trace detection
limits is required. It has a simplified
maintenance and easy to clean without
tools. Also, it has the flexibility to
choose the ideal level of sensitivity and
dynamic range, and software for easeof-use from data collection for
evaluating and reporting. Also, sample
handling system must be included.
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 12
Department of Applied Sciences – Faculty of Engineering
Technology: Instrumentation for Instrumental analysis, Analytical
Chemistry, Organic Chemistry and General Chemistry Laboratories
Educational with suitable set of
experiments to be demonstrated.
- Original operating manual
- Full training programme on running
the equipment and maintenance.
-- Manufacturer must be from either
Jordan, USA, UK, Europe or Japan
-
-.
Origin for all equipment should be USA, Europe, Japan unless otherwise indicated
- Operating manual + Maintenance manual.should be included
- Spare parts and all required material for proper operation of the instruments should be included.
- Certificate of origin is required.
Department of Applied Sciences – FETAl-Balqa Applied Univesrity
Page 13