NATIONAL INSTITUTE FOR PLANT GENOME RESEARCH
(Automated DNA Sequencing Facility)
Date……………..
Indentor………………......................
Email….…………………………….
Phone number...…………………….
Name of Scientist ………………………………
Lab No...……………………………
Please fill this table while adding the DNA Samples in the reaction plate
1
2
3
4
5
6
7
8
9
10
11
12
A
B
C
D
E
F
G
H
Plasmid DNA Samples - 2μl (75ng/μl) to be provided by indentor .Own primers must be provided by the
indentor.
Number of DNA samples to be sequence with M13 Forward primer
= ……………………….
Number of DNA samples to be sequence with M13 Reverse primer
= ……………………….
Number of DNA samples to be sequence with Own (Gene specific) primer
= ……………………….
Total number of DNA samples
= ………………………..
Signature of Scientist …………………………………………. …………………………
Indentors signature after loading DNA samples in the reaction plate
…………………………..
REGULATIONS OF USING DNA SEQUENCING FACILITY
1) Requisitions for the number of sequencing reactions has to be entered in the logbook (Do
not mention the primers).
2) Altogether at least total of 90 samples are required to run a plate. Until that the indenter has
to wait.
3) No change will be accepted after the entry in the log book (over writing etc.).
4) In case of any change a fresh entry has to be made.
5) Indents have to be submitted during the entry in the booking logbook.
6) Indents must be signed by any of the faculty members. (Photocopy of signature is not
allowed).
7) DNA samples have to be loaded within 12 noon on the day of sequencing.
8) It will be understood that booking in the log book for sequencing means DNA samples are
ready.
9) In case of unavailability of indent form or DNA samples during the sequencing, booking will
be cancelled for next two sequencing schedule.
10) No trainee and /or accompanying persons are allowed in the DNA Sequencing Facility to
minimize crowd while loading.
11) The sequences will be permanently deleted from both the computers associated with the
sequencer within seven days of sequencing.
12) DNA Sequencing Facility is not responsible for keeping and / or providing the sequences
after seven days of sequencing.
Due acknowledgement be given to DNA Sequencing Facility, NIPGR, in the research publication
emerging out of the work carried out at this facility.
Arun Kumar
Debasis Chattopadhyay
Technical Officer
DNA Sequencing Facility
NIPGR
Staff Scientist
DNA Sequencing Facility
NIPGR