ANIMALS TO BE BRED BY THE PRINCIPAL INVESTIGATOR
& TO BE USED FOR SUBSEQUENT EXPERIMENTATION
FORM 6
Please indicate separately the no. of breeders and stock animals for each strain/line listed below and attach
separate A-4 size sheets if necessary.
“Breeder” refers to the estimated number of male and female breeders in the breeding colony at any one time.
“Reserve Breeder” includes those animals (male and female) set aside in order to reach maturity so that they
can be used as future breeders in order to maintain the breeding colony.
“Stock” refers to the weaned offspring in the breeding colony at any one time.
[note: these weaned animals, include all offspring which will be used in the experiments and surplus animals
which are unsuitable for use in the experiments]
A.
Brief description of breeding
programme
(please include a justification for this
breeding programme)
B.
Breeding Animals
i.
ii.
iii.
iv.
v.
vi.
C.
Species
Sex (indicate number of males
and females)
Age
Special characteristics
Total number of breeding pairs
including reserve breeders and
stock animals
Justification of numbers of
breeders
(e.g. providing reason for
number of breeding cages,
groups of reserve breeders and
stock animals, etc.)
Location
(Please note that prior approval of
animal facility manager is required)
i.
Location of breeders
(and reserve breeders)
Bldg
Room
ii.
Location of stock
animals
Bldg
Room
D.
Breeding Programmes
(Please attach a breeding flowchart
for the generation of modified
animals)
i.
Please describe the breeding
programme to be performed, list
as 1, 2, 3 etc.
ii.
Include the likelihood of either
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an undesired or desired
phenotype/genotype at each
breeding procedural step.
iii.
Please provide an estimate of
the total number of stock being
held at any one time (see above
for definition of “stock”
animals).
E.
iv.
Please allocate a strain name or
code for the
phenotype/genotype generated
by this breeding programme, for
ease of reference and
identification.
v.
Severity:
[U] Unclassified
Animal Welfare
(for both the breeding, reserve
breeding, and stock animals – please
list separately as required)
i.
How often will the animals be
monitored for their well-being?
ii.
Person(s) who will monitor wellbeing of animals
(please give the name and post
of staff / student and contact
phone no.)
iii.
Will there be phenotypes /
genotypes that limit the ability of
the animal to move freely, feed
and groom itself?
Please explain!
F.
G.
H.
Humane Endpoints
(for the breeding, reserve breeding
and stock animals – please list
separately as required)
If animals are found to be suffering
during the course of this breeding
programme under what conditions
the animals will be terminated?
Please confirm whether offspring will
be used for short-term and/or
long-term and attach appropriate
Forms 1, 2, 3, 4, 5 and/or 6.
Termination
(for both the breeding, reserve
breeding, stock and experimental
animals – please list separately as
required)
Clearly describe method to be used.
I.
Justification if non-standard
euthanasia method used
J.
Disposal method for carcasses,
tissues and contaminated materials
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K.
Disposal method for live animals
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