IISc
WORKSHOP
ON
Molecular Medicine: Expression of Recombinant Antigens
March 24-31, 2006
Organized by
ICMR Centre for Advanced Research in Molecular Medical
Microbiology
Department of Microbiology and Cell Biology
Indian Institute of Science
Bangalore-560012, India
The workshop includes lectures and hands on training on cloning and gene
expression molecular techniques. This workshop aims to introduce young medical
graduates (MBBS/MD) to the emerging areas of molecular medicine so as to widen
their research activities in their respective centers. The details of the workshop and
the course contents will be available at the department home page at
http://mcbl.iisc.ernet.in/
Interested candidates may apply to Prof. C Durga Rao with their bio-data (include
email / fax number), statement of purpose and a letter of recommendation from
their supervisor/head of the division) by February 15, 2006. The selected candidates
will be informed by email/fax/speed post by February 25, 2006.
FACULTY
Prof. K. P. GOPINATHAN
Prof. R. NAYAK
Prof. M. S. SHAILA
Prof. C. DURGA RAO
Prof. V. NAGARAJA
Prof. U. VARSHNEY
Prof. S. VIJAYA
Prof. U. VIJAYRAGHAVAN
Prof. P. SADHALE
Prof. S. DAS
Prof. P. AJIT KUMAR
Prof. K. SOMASUNDARAM
Dr. U. NATH
Dr. K .N. BALAJI
Dr. D. CHAKRAVORTTY
Dr. S.S. INDI
Prof. C. Durga Rao
Prof. R. Nayak
Course Co-ordinator
Phone: 080 2293 2415
Email: workshop@mcbl.iisc.ernet.in
Programme Co-ordinator
Phone: 080 2293 2703
Fax: 080 2360 2697
The Workshop Contents:
The workshop includes a series of lectures by the faculty of the department
and hands-on training on basic techniques involving molecular cloning and
expression of the recombinant antigens. The techniques includes: RT-PCR,
PCR, Purification and digestion of the PCR DNA with restriction
endonucleases, agarose gel electrophoresis, purification of the DNA
fragments from ogarose gels, ligation of PCR fragments into pBluescript
plasmid vector, preparation of competent E.coli HB101 cells, transformation
of E.coli HB101 with ligated DNAs, selection of recombinant clones,
preparation of miniprep plasmid DNA, purification of the inserts and
ligation into pET22 expression vector.
Transformation of E.coli BL21
(DE3), Analysis of recombinant clones for DNA insert, protein expression
by
SDS-PAGE,
purification
of
recombinant
protein
by
affinity
chromatography using Ni-NTA agarose, SDS-PAGE of purified proteins and
western blotting and detection by specific antibodies.