IntAct
COMMON ELEMENTS
ac := idnumber for IntAct database
shortLabel :=
for proteins – UniProt Ids
for experiments – author-year-number (author-1st author, year-of publication, number-if
multiple instances)
for interactions – gene1-gene2 (not always stable, not always unique, always lowercase)
fullName := full name of the object
xref := crossreference to other databases
name := name of external db
primary id := primary id or accession number of object in external database
secondary id := secondary id of object in external db
crossreference type := type of the crossreference
annotation := (0..n) has topic to broadly classify the annotation, and text
created := date the object has been created
updated := last updated date
MAIN OBJECTS
Experiment

BioSource := biological system in which the interaction took place in the experiment

identificationMethod := experimental method by which the molecules participating in the
interaction have been detected (ex: mass spectrometry)
http://www.ebi.ac.uk/ontologylookup/browse.do?ontName=MI&termId=MI%3A0002&termName=participant%20identification%20
method

interactionMethod := experimental method by which the interaction has been detected (ex: yeast
two hybrid)
http://www.ebi.ac.uk/ontologylookup/browse.do?ontName=MI&termId=MI%3A0001&termName=interaction%20detection%20meth
od

interactions (1..*)
Interaction

interactors (2..*)
CvInteractionType := description of the type of interaction (ex: association)
http://www.ebi.ac.uk/ontologylookup/browse.do?ontName=MI&termId=MI%3A0190&termName=interaction%20type

kD := dissociation constant

Interactor

CvInteractorType := type of interactor as defined in PSI-MI ontology (ex: protein, DNA, small
molecules)
http://www.ebi.ac.uk/ontology-
lookup/browse.do?ontName=MI&termId=MI%3A0313&termName=interactor%20type

BioSource := biological system in which the interactor is normally found (may not be the only
system in which it is found)
[if a polymer]

sequence := amino acid sequence of the protein

crc64 := the CRC64 checksum for the amino acid sequence

stoichiometry := number of units of this interactor which participate in the interaction (default = 1)

expressedIn := a BioSource object describing the biological system in which the protein has been
expressed (ex: if a human protein has been expressed in E. coli, it would be E. coli)

role := the role of the Interactor in the Interaction (ex: Y2H would have bait and prey)

features := properties of the molecule that is involved in the interaction
[endif]
AUXILIARY OBJECTS
BioSource





taxId := NCBI tax id (identifies an organism)
shortLabel := UniProt OS code (optional)
fullName := scientific name of the species
tissue := tissue type used in the experiment (optional)
compartment := cellular compartment in which the interaction has been shown to occur
CvObject
Features