Plants and People: Nutrition and Technology Grade level: 7-9 State Standards Concepts and processes: Life systems -Levels of organization in living systems -Diversity of organisms History and nature of science in personal and social decisions Student background knowledge -flower parts and reproduction -monocots and dicots, -hydroponics - plant nutrition. Day 1 “You are What you Eat”: Human Nutrition and the World of Plants Time: 60 minutes Goals Students will understand that there is an abundance of plant diversity with agriculture representing a few important families Students will understand that the functions of various plant parts/organs tie closely to human nutrition Students will understand that the commonalities and differences of plants are both determined by genetics that have changed over millions of years. Objectives Students will be able to describe examples of plant organs and the common characteristics of these anatomical parts Students will determine how characteristics of each of these parts fit into human nutrition. Students will be able to analyze scientific materials to gain knowledge and understanding of a topic Guiding Questions What parts of a plant do we rely on for food and energy? If these plant parts are useful to us, how are they also useful to the plant? Is plant diversity natural or man-made? Engage Choose a plant family with particular relevance to the region where the school is located. For example, Lovell, Wyoming is also known as “Rose City” because this shrub grows well in the climate. Roses are in the family Rosaceae. Does Rosaceae just refer to the flowers we call roses? How many types of rose ARE there? Draw classification scheme on the board: Kingdom Plantae -- Plants Subkingdom Tracheobionta -- Vascular plants Superdivision Spermatophyta -- Seed plants Division Magnoliophyta -- Flowering plants Class Magnoliopsida -- Dicotyledons Subclass Rosidae Order Rosales Family Rosaceae -- Rose family Genus Rosa=garden roses What are some other plants in the Rosaceae family? Show some examples of variations within this family. Members of the rose family: rose, serviceberry, strawberries, raspberries, cinquefoil, mallow ninebark, cherries, plums, thimbleberry, spiraea (avens), almonds, california holly (“Hollywood”), pears, apricots, peaches.... Pass around a few examples/snacks of plants within the plant family chosen. Examples of Rosaceae plants: almonds, strawberries, apple slices, rose and rose hips. What plant parts do we humans use from members of the rose family besides just the pretty flowers of Rosa….? Make a big list on the board (should include fruit, seeds/nuts, leaves, etc. as well as flowers). Explore Students should brainstorm and write down the similarities and differences between these important plant parts: what are the necessary features of each of these plant parts and why? Explain To review a little bit, have students work with the person next to them to draw out the major steps of a life cycle of a plant, making sure that all of the plant parts listed on the board are incorporated somehow. Students should feel free to use their textbooks or notes from previous classes. Humans use plants in different ways based on what part of the plant we want and how we can use it. Different plant parts have different nutritional values…but WHY? Revisit functional parts of a plant, or organs (humans aren’t the only one’s with an anatomy and specific organs!) Elaborate Students will take on the role of plant biologists working to communicate their understanding to the public. Students will work in groups of 3-4 to develop an advertisement for one of the major organs or functional parts of a plant. The goal is to market the particular plant part as an important addition or contribution to our daily diet. Students should think about what their plant part can contribute that is essential to human nutrition and health. Students will be assigned to one of the following: -Roots and tubers -Shoots and leaves -Flowers -Fleshy fruits -Seeds and hardened seed coverings Teacher will hand out specific student resources to each group, including a rubric for the short project. Groups will have 20-25 minutes to work through materials and design advertisements. Advertisement should contain: a diagram of where and how this particular organ fits into the biology or life cycle of an angiosperm plant. The finished product should be interesting and convince people that they should include it in their diet or that this plant part makes important contributions to human health and nutrition. Evaluate Students will present their advertisements to the class. Teacher will assess work using the attached rubric. Time for question-and-answer between student groups. Wrap-up Revisit guiding questions. Both man-made and natural plant diversity influence how we use plants. We rely on some plants more than others. What plant part have humans come to rely on most? Is diversity within our diet still important? Some other plant families are important to human civilization. Fit Brassicaceae (cabbage, mustard, turnips), Poaceae, and Fabaceae (peas, beans), etc. into the classification scheme on the board. Materials: various examples (edible or non-edible) of a particular plant family, background info/diagrams to hand out to students, project rubric, poster board, markers/colored pencils, colored and white paper. Day 2 Frankenfoods or Miracle Crops? Goals Students will understand the basic principles that enable scientists to create genetically modified foods Students will understand the various perspectives surrounding the production and use of genetically modified plants Objectives Students will describe the important steps in creating a transgenic plant Students will create research questions based on background knowledge of biotechnology Students will be able to critically read scientific material, analyzing for bias and perspective in scientists’ conclusions Guiding Questions What are genetically modified plants and how are they different from other plants? Why are scientists creating transgenic plants? Are genetically modified foods bad for you? Background: Biotechnology does not equal genetically modified foods. Biotechnology applies to the crop breeding that has been going on for 8 thousand years as well as grafting, tissue culture and other manipulations of plants and animals. One of the main purposes of genetic modification is to improve crops in a more financially or time efficient manner. More specifically, purposes of creating GM plants include disease resistance, drought or cold tolerance, salinity tolerance, and increased ability to take up nutrients. Another main purpose is to create useful pharmecuticals or by-products from plants (more basic biotechnology). Some GM plants produce plastics (Michigan scientists developed plant that produces polyhydroxybutyrate). Also we commonly see biopesticides developed in transgenic plants. For example, Bacillus thuringiencis compounds are expressed in corn and potato. New horizons in the field of transgenic plants include nematode-bacteria complexes that kill insects via infection. Many transgenic plants are produced via culture tissue with bacteria Agrobacetrium tumefaciens (the bacteria that normally causes crown gall disease in plants). A. tumefaciens carries the gene of choice and inserts it into the plant cells nucleus, where it is taken up by the plant DNA. This bacteria is naturally “promiscuous” and inserts its DNA into the plant and the plant begins to express it. Other transgenic plants, particularly the monocots are transformed with a gene gun. This alternative, while not as effective, is necessary for grain crops because A. tumefaciens does not infect it. It is important to keep in mind that expression of genes is something regulated at many levels- it is influenced by hormones, enzymes, chemicals, etc.. In a strand of DNA, genes are preceded by a promotor and operator. The promotor signals transcription of RNA and the operator regulates genes. Process of creating genetically modified plants: -isolate the gene you want (mapping) -make lots of copies of it (to be on the safe side, and so you have a lot to work with) (PCR) -add a promotor to make sure it is expressed (usually this is an altered form of cauliflower mosaic virus, found naturally in Brassicas…one risk is that this promotor leads to recombination, a possible risk. -transfer DNA to totipotent plant cells using a “gene gun” or soil bacteria -check to make sure gene made its way into DNA of plant and is being expressed (via marker gene that causes chemical signal, fluorescence, or antibiotic resistance) Engage Tie today’s lesson to yesterday’s lesson with story about one example of a genetically modified plant that has received a lot of attention: transgenic fruit with a fish gene. Why did scientists develop this plant? Is it part fish? How is this process similar and different to crop breeding that produced commercial strawberries from small tart wild strawberries? In general, there are a lot of myths out there, as well as a lot of unanswered questions. We will investigate some of these issues for the rest of the period. Explore In groups, students will rotate throughout the room, visiting exploration stations. Students will spend 5-10 minutes at each station, taking notes along the way. Station topics include: 1. purpose(s) of creating genetically modified plants Teacher will provide real world examples of various genetically modified plants with a short explanation. Ask students: Why would scientists develop genetically modified plants? Can you think of 3 roles these plants play based on the examples provided here? (for resources see references below) 2. Are genetically modified foods bad for you? 3. Steps in the process Teacher will provide computer with internet access. Students will complete the simulation of creating a transgenic plant from the “Harvest of Fear” website created by Frontline and NOVA (http://www.pbs.org/wgbh/harvest/engineer/). 4. DNA and a plant cell under the microscope Students will try to organize various pictures from largest to smallest in scale to help them understand how genetic expression fits into a cell -plant leaf -plant cell -cell nucleus -chromosomes -DNA double helix Explain Students will regroup and debrief the exploration stations as a class. Elaborate Students will receive one of two short articles about genetically modified foods. Students will read the materials carefully and then discuss the pros and cons of genetically modified foods with each other. Evaluate Based on the exploration stations and two articles, students will describe the various perspectives or biases expressed in the articles and brainstorm 3 questions they believe still need to be addressed concerning genetic modification of plants. What science do these authors rely on? What plant science is missing? Wrap-up Genetic modification of plants is a very exciting field of science and requires the participation and knowledge of the public and responsible action on the part of the scientists. If time, teacher will show students a quick video of plants with firefly fluorescing gene linked to expression of circadian rhythm genes. For final assessment, have students take turns explaining the steps in the process that led to this amazing event! References Armstrong, W.P. 2007. Wayne’s Word: 15 July 2007. http://waynesword.palomar.edu/ecoph17.htm (8 October 2007) Chrispeels, M.J., and Sadava, D.E. 1994. Plants, Genes, and Agriculture. (available online). Jones and Bartlett Publishers: Boston. Chrispeels, M.J., and Sadava, D.E. 2003. Plants, Genes, and Crop Biotechnology. Jones and Bartlett Publishers. Colorado State University. “Transgenic Crops: An Introduction and Resource Guide”. (http://cls.casa.colostate.edu/TransgenicCrops/history.html) PBS. 2001. “Harvest of Fear”. (http://www.pbs.org/wgbh/harvest) Taiz and Zeiger (2002). Plant Physiology (3rd ed.) USDA, Natural Resources Conservation Service: Plants Database (http://plants.usda.gov/java/ClassificationServlet?source=display&classid=Rosacea e) Young, P. 1982. The Botany Coloring Book. Coloring Concepts, Inc. Oakville, CA.