HIBBING COMMUNITY COLLEGE
COURSE OUTLINE
COURSE TITLE & NUMBER: MLT 2450: Hematology 3
CREDITS: 2
PREREQUISITES: None
CATALOG DESCRIPTION:
Hematology 3 introduces students to the principles and disorders of hemostasis
and thrombosis, reviews special procedures in hematology and coagulation,
hematology instrumentation and analysis of instrumental data.
OUTLINE OF MAJOR CONTENT AREAS:
I.
II.
III.
IV.
V.
VI.
Principles of Hemostasis and Thrombosis
Blood Coagulation Factors
Disorders of Hemostasis and Thrombosis
Laboratory Assessment of Coagulation Deficiencies
Review of Special Procedures in Hematology
Instrumentation in Hematology
COURSE GOALS/OBJECTIVES/OUTCOMES:
The student will
1. explain how vasoconstriction participates in hemostasis.
2. explain the function of platelets in response to vascular damage.
3. list the steps in platelet plug formation and describe the process of platelet
adhesion and aggregation; describe the sequence of events that leads to
fibrinolysis.
4. cite at least two symptoms of thrombocytopenia.
5. describe the laboratory tests that may be used to evaluate platelet
function: Ivy bleeding time, Duke bleeding time, clot retraction, platelet
aggregation testing.
6. list the principal coagulation factors.
7. describe the sequence of events in the extrinsic pathway.
8. describe the sequence of events in the intrinsic pathway.
9. explain the uses of oral warfin therapy and the effect this anticoagulant
has on coagulation tests.
10. discuss the principle prothrombin time (PT) test including reagents used
and their major components, methodology, coagulation factors measured,
and normal reference range.
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11. discuss the purpose of the International Normalized Ratio (INR) for
reporting prothrombin time test results for patients who are undergoing
oral anticoagulant therapy.
12. compare prolonged prothrombin times with clinical conditions and/or
disease states.
13. explain the uses of therapeutic heparin and the effect this anticoagulant
has on coagulation tests.
14. discuss the principle of the activated partial thromboplastin time (APTT)
test including reagents used and their major components, methodology,
coagulation factors measured, and normal reference range.
15. compare prolonged activated partial thromboplastin times with clinical
conditions and/or disease states.
16. explain the principle of specific coagulation factor assay tests.
17. discuss the principle of thrombin and fibrinogen testing.
18. discuss theory and principle of D-dimer testing.
19. compare and contrast the principles of the laboratory tests used most
frequently to diagnose von Willebrand's Disease, Bernard-Soulier
syndrome and Glanzmann’s thrombasthemia.for bleeding time, PT, APTT,
factor VIII:C assay, and platelet aggregation studies.
20. compare patterns of inheritance for hemophilia A, hemophilia B, and
hemophilia C and state the specific factor deficiency for each disorder.
21. explain the effect of circulating factor VIII:C antibodies on hemophilic
patients.
22. discuss the process of disseminated intravascular coagulation. List
common clinical conditions associated with DIC.
23. compare and contrast the type of expected coagulation results (D-dimers,
PT, APTT, thrombin time, platelet count, fibrinogen) in patients with DIC.
24. explain why examination of a peripheral blood smear is important when
DIC is suspected.
25. discuss the laboratory evaluation of AT-III deficiency.
26. identify risk factors associated with the hypercoagulability state.
27. discuss different endpoint detection methodologies used to detect clot
formation by coagulation analyzers.
28. explain the significance of improperly filled sodium citrate (blue top) tubes
and the proper steps to be taken when this situation arises.
29. discuss the principle and procedure for performing: total leukocyte count,
manual hematocrit, platelet estimates, ESR, Donath-Lansteiner screening
test for PCH, sucrose hemolysis test for PNH, osmotic fragility test,
dithionite tube test, and Kleihauer-Betke stain.
30. describe the basic theory of the electrical impedance principle of cell
counting and sizing.
31. describe the basic theory of the optical detection (light scatter) principle of
cell counting and sizing.
32. describe the appearance of microcytic and macrocytic erythrocytes on a
histogram.
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an equal opportunity educator & employer
33. name two conditions that would contribute to a bimodal cellular
distribution on an erythrocyte histogram.
34. describe the appearance of a leukocyte histogram.
35. analyze and discuss common errors and flags regarding automated
hematology analyzers.
36. apply prior knowledge to problem solving.
 recognize abnormal or unusual test results.
 recognize unacceptable quality control results.
 apply knowledge of established procedures to verify test results
LAB COMPETENCIES:
The student will
1. demonstrate the performance of a PT/PTT using automated
instrumentation and acceptable/unacceptable QC interpretation
2. calculate the INR from the prothrombin time in seconds and ISI, and
interpret the results are normal or therapeutic or panic values
3. perform an ESR and interpret results as normal/abnormal
4. interpret histogram results of automated instrumentation, with resolution of
flagged parameters
5. demonstrate use of reference laboratory procedure manuals and
specimen collection for special coagulation testing procedures
6. demonstrate the recognition of significant normal and abnormal results in
case studies
7. demonstrate safe and professional work habits
STUDENT CONTRIBUTIONS:
Students are expected to complete assignments on time and spend the
necessary study time to pass all exams.
METHODS FOR EVALUATING STUDENT LEARNING:
Performance appraisals, unit tests, assignment completion, lab reports, and a
final exam are methods used for evaluation.
11/16/04
Date Approved
Hibbing Community College, a technical & community college
an equal opportunity educator & employer