GENETICS AND MOLECULAR BIOLOGY
(BIOLOGY 218) - Spring 2005
Professor
:
Dr. George Mourad
Textbook
:
GENETICS: from Genes to Genomes
Authors: L.H. Hartwell, L. Hood, M.L. Goldberg, A.E.
Reynolds, L.M. Silver, R.C. Veres
Second Edition, 2004
Office
:
Science Building, Room 380
Phone: 481-5704
E-mail: mourad@ipfw.edu
Web site:
http://users.ipfw.edu/mourad/Courses/courses.htm
Office hours :
Monday
10:30 - 11:30 a.m.
Wednesday 10:30 - 11:30 a.m.
Friday
3:30 - 4:30 p.m.
Or by appointment
Grading and evaluation:
There is a total of 600 points assigned for this course. The lecture material is worth 450
points (75%) and the laboratory part is worth 150 points (25%). For the lecture material,
there will be four exams. The first three exams are not comprehensive. The final exam
will be partially comprehensive.
The distribution of points is as follows:
Exam I
Exam II
Exam III
Exam IV
Laboratory
Total
Grading Scale:
= 100 points
= 100 points
= 100 points
= 150 points
= 150 points
= 600 points
> 88% (528/600 pts)
A
77% (462/600 pts) - 87% (522/600 pts)
B
66% (396/600 pts) - 76% (456/600 pts)
C
55% (330/600 pts) - 65% (390/600 pts)
D
< 54% (324/600 pts)
FAttendance
is expected and will be taken each lecture. Attendance will be used only to help you if
you are at a border line between two grade categories (B/A, C/B or D/C). In other
words, if you attended 42 out of the 45 lectures and you need one percent to change
your grade from B to A then you will have this point. The same applies to C/B and D/C
border line grades. In previous years, several students benefited from this policy.
VERY IMPORTANT: Makeup exams are not allowed except for strong medical reasons
documented by a medical certificate from your physician or for a strong personal
reason that will be subject to my approval. A makeup up exam for the previous reasons
will be allowed only ONCE during the semester (NO makeup for the final exam). You
should call or e-mail me BEFORE the exam if possible and arrange with me for a time
to take the makeup exam within 48 hours following the originally scheduled exam.
Laboratory:
The laboratory part of this course will consist of two main parts. In the first part you will
do experiments dealing with classical genetics including Mendelian monohybrid and
dihybrid crosses in the fruit fly Drosophila melanogaster, three-point mapping in
Drosophila melanogaster, allozyme variation in fish and natural selection in fruit flies.
Classical genetics experiments will take the first half of the semester and will be taught
by Dr. Robert Gillespie. In the second part of the semester, immediately following spring
break, you will do experiments dealing with molecular genetics and genetic engineering.
You will spend the second half of the semester doing molecular genetics experiments
and will be taught by Dr. George Mourad. Molecular experiments include genetic
engineering of bacteria with an unknown plasmid DNA molecule. You will identify the
plasmid molecule by purifying plasmid DNA from the transformed bacteria, digesting the
plasmid DNA with endonuclease restriction enzymes and do DNA agarose gel
electrophoresis. You will also isolate chromosomal DNA from a small plant
(Arabidopsis thaliana) and clone different genes using the technique of polymerase
chain reaction (PCR) followed by ligation into different vectors.
Date
Jan 10
Jan 12
Jan 14
Topic
Introduction: The Study of Biological Information
Mendel’s Principles of Heredity
Mendelian Inheritance in Humans & Solving Problems
Chapter
1
2
2
Pages
1-12
13-28
29-42
Jan 17
Jan 19
Jan 20
HOLIDAY (Martin Luther King, Jr.)
Extensions to Mendel: Gene Interactions, Multiple alleles
Epistasis
3
3
43-53
53-68
Jan 24
Jan 26
Jan 28
Solving Problems
3
The Chromosome theory of Inheritance (Meiosis & variation) 4
Sex Determination & Solving Problems
4
69-75
77-95
95-112
Jan 31
Feb 2
Feb 4
Gene Linkage & Mapping
Solving Problems
Review
5
5
113-144
145-154
Feb 7
Feb 9
Feb 11
EXAM I
Structure of Genetic Material
DNA Replication & Recombination
6
6
155-169
169-190
Feb 14
Feb 16
Feb 18
Review
Molecular Basis of Mutations
Mutations Reveal Gene Structure (Complementation)
7
7
191-206
206-213
Feb 21
Feb 23
Feb 25
Mutations Reveal Gene Function (Biochemical Genetics)
Review
Gene Expression: The Genetic Code
7
213-227
8
237-247
Feb 28
Mar 2
Mar 4
Gene Expression: Transcription (Prokaryotes Eukaryotes)
Gene Expression: Translation (Protein Synthesis)
Review
8
8
247-256
256-275
Mar 7
Mar 9
Mar 11
Spring Break
Spring Break
Spring Break
Mar 14
Mar 16
Mar 18
The Eukaryotic Chromosome: Packaging
EXAM II
DNA Packaging & Gene Expression
12
415-428
12
428-440
Mar 21
Mar 23
Mar 25
Catch Up
Chromosomal Rearrangements
Transposable Elements
13
13
441-460
460-466
Mar 28
Mar 30
Apr 1
Numerical Chromosomal Aberrations
Bacterial Genetics
Gene Transfer in Bacteria
13
14
14
466-486
487-496
496-511
Apr 4
Apr 6
Apr 8
Non-Mendelian Inheritance: Organelle Genomes
Mitochondrial & Chloroplast Genetics
Review
15
15
525-535
535-550
Apr 11
Apr 13
Apr 15
EXAM III
Gene Regulation in Prokaryotes: at Transcriptional Level
Gene Regulation in Prokaryotes: Attenuation & Others
16
16
551-567
567-572
Apr 18
Apr 20
Apr 22
Gene Regulation in Eukaryotes: at Transcriptional Level
17
Gene Regulation in Eukaryotes: at Transcriptional (Cont’d) 17
Gene Regulation in Eukaryotes: at Post-transcriptional Level 17
581-592
592-602
602-615
Apr 25
Apr 27
Apr 29
Developmental Genetics
Developmental Genetics (Cont’d)
Review
649-665
665-672
19
19
May 2
EXAM IV (8:00 am - 10:00 am)
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