Molecular Biology Services
DNA Sample Preparation &
Analysis Order Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
Instructions:
* Denotes required fields
CUSTOMER DETAILS
*Name:
Reference ID:
*Institute Name / Department
*Address:
*Contact Number:
*Email Address:
(Office)
(Mobile)
Principal Investigator / Supervisor:
DNA PREPARATION / ANALYSIS (Please indicate with a X)
Type of Service
A.
PREPARATION
Min. sample amount /
reaction required
Remarks
Genomic DNA Extraction
Project dependent
Specifically for subsequent PCR
optimization. Please enquire for others
DNA Digestion using Restriction Enzyme(s)
5 g of genomic DNA /
plasmid DNA
Excludes gel extraction. Please enquire
if gel extraction is required after RE
digestion
Dephosphorylation of Linear Vector DNA and Gel Purification
5 g of plasmid DNA
DNA Shearing by Sonication and PCR Clean-up.
10 g of genomic DNA
Please select the peak size:
(1) 150 (
)
(2) 200 (
)
(3) 300 (
)
(4) 500 (
)
(5) 800 (
)
(6) 1000 (
)
(7) 1500 (
)
Blunting and Phosphorylation of Double-stranded DNA
(100 – 2500 bp) and PCR clean-up
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5 g of DNA fragments
Excludes gel extraction. Pls enquire if
gel extraction is required after DNA
shearing
Molecular Biology Services
DNA Sample Preparation &
Analysis Order Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
DNA PREPARATION / ANALYSIS (Please indicate with a X)
DNA Digestion using Restriction Enzyme(s)
B.
ANALYSIS
DNA LabChip
DNA, double-stranded, fragmented, size 25 to 7500 bp
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
1 g of genomic DNA/
plasmid DNA
I. 5 l of DNA fragments
II. concentration: 0.5 to 50
ng/l
Supercoiled dsDNA needs to be
linearized before this service
SAMPLE INFORMATION (Note: for larger samples, please attach a separate sheet with details as requested in the table)
#
S/N
*Sample Name
Organism
*Sample Type
Concentration
Purity
Volume
Special
(1) Genomic DNA
OD260/280
Instructions/Protocols/ Result
(ng/l)
(l)
(2) Plasmid DNA
Requirements
1.
2.
3.
4.
5.
* Required fields
# Please indicate if you have any specific instructions/protocols/result requirements, attach as separate sheets if necessary
Have I met the sample preparation requirements?
Checklist (Please indicate with a X):
Submitted minimum required amounts of DNA for one reaction.
Purified DNA is in either TE or 10 mM Tris-HCl (pH 8.0) elution buffer. Its purity OD260/280 = 1.8 – 2.0
Prepared samples in 1.5/ 0.5ml microcentrifuge tubes with at least 10 l of contents. Each tube should be clearly labeled using a permanent marker,
with caps sealed with parafilm. For optimal analysis results, Genomic DNA samples, Plasmid DNA and PCR products are recommended to be shipped
at 4oC.
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