Legends to Supplementary Figures for 409 1007
Figure 1. Genome map. Linear map of the chromosome of the TN strain of M. leprae
showing the position and orientation of known genes, pseudogenes and CDS. Genes above
the lines are transcribed from left to right, those below from right to left. Pseudogenes are
depicted as hatched, white rectangles while genes are colour coded using the following
functional categories: Lipid metabolism (dark grey); Intermediary metabolism and respiration
(yellow); Information pathways (red); Regulatory proteins (sky blue); Conserved hypothetical
proteins (orange); Proteins of unknown function (light green); Proteins specific for M. leprae
(light green with red tag); Insertion sequences and phage related functions (blue); Stable
RNAs (dark blue); Cell wall and cell processes (green); PE and PPE protein families
(magenta); Virulence, detoxification, adapatation (white). Repetitive DNA is indicated by
blue rectangular boxes situated on the central lines. For additional information about gene
functions
consult
the
websites
http://genolist.pasteur.fr/Leproma/ ).
(http://www.sanger.ac.uk/Projects/M_leprae
or
Recombination between rep-DNA
A
Rv1056
B
Rv0409, Rv0408
(ackA, pta)
ML 0266 REPLEP ML0267, ML0268
C
Rv1817
Rv3361c
Rv2521 (bcp), Rv2520
ML0423 RLEP
ML0424 (bcp), ML0425
D
Rv1115
ML0934 LEPREP ML0939, ML0940
Rv2601
(speE)
ML0476’ RLEP ‘ ML0476
(speE ’ ‘ speE)
Figure 2. Repetitive elements and genome discontinuities. The three main repetitive elements
in the M. leprae genome are shown together with examples of flanking genes and their
counterparts in M. tuberculosis. Note the breaks in continuity of numbering of M. tuberculosis
genes that indicate translocation events. A. REPLEP, 12 complete copies of ~875 bp, plus 2
fragments; B. RLEP, 36 complete copies of 545 – 700 bp plus 1 fragment; C. LEPREP, 6
complete copies of 2,400 bp plus 3 fragments. D. Example of gene disrupted by RLEP, speE,
spermidine synthase.
Electron donors
(other substrates)
Dehydrogenases
Electron transfer
from quinone
Terminal
Reductases
Terminal
electron
acceptors
H+
NDH I
NADH
Translocation
nuoA-N
ndh
Succinate
Succ DH
Glycerol-3-P
sdhABCD
Quinone pool
?
NDH II
G-3-P DH
gpdA
H+
QcrABC
Cytochrome
c oxidase
H+
O2
cta/ccs
G-3-P DH
(Glycerol)
glpD1,2
NarI
PDH
aceE-Rv0462
pdhA,B,C-lpdA
H+
Nitrate
narGHJ
(Pyruvate)
FrdCD
LDH*
lldD2?
Lactate*
Nitrate
reductase A
Lactate oxidase
Fumarate
reductase
H+
Fumarate
frdAB
lldD1?
Formate
Formate DH**
hyc/fdhD,F
Figure 3. Comparison of predicted electron transfer pathways in M. tuberculosis and M.
leprae. Genes and proteins that are functional in both mycobacteria are shown in black
lettering whereas those that are present as pseudogenes in M. leprae but functional in M.
tuberculosisare in red. Abbreviations (where not defined): G-3-P DH, glycerol-3-phosphate
dehydrogenase; PDH, pyruvate dehydrogenase; LDH, lactate dehydrogenase; NDH I and
NDH II, NADH dehydogenases I and II; Succ DH, succinate dehydrogenase. Gene names
conform to standard genetic nomenclature.