Phosphatidyl Serine (PS) Exposing Red Blood Cells
Phosphatidyl serine (PS) exposure has been shown to be increased in disease
states such as sickle cell anemia and thalassemia (ref: deJong, year).
A sample of erythrocytes is incubated with a fluorescently labeled dimer of the
annexin V molecule, which binds specifically to PS in the presence of calcium.
Any fluorescent events detected by flowcytometry are determined to expose PS
and are counted as positive events.
Phosphatidyl serine exposing cells are represented as the percent of the
population that binds annexin V for sickle cell disease patients (SS) and normal
controls (AA).