Supplementary Material (ESI) for Journal of Materials Chemistry
This journal is © The Royal Society of Chemistry 2001
1. The histogram for Mg-solubility of Mg2Al-LDH as function of pH.
In order to describe the pH dependent Mg2Al-LDH solubility, it has been
measured that the amount of Mg ion resolved from Mg2Al-LDH in each pH aqueous
solution after 24hrs. We can theoretically expect that LDHs will be dissolved in
endosome (pH 4~5) or cytosol.
100
80
S/%
60
40
20
0
1
2
3
4
5
6
7
pH
Figure S-1. The amount of Mg ion resolved from Mg2Al-LDH for 24 hrs at room
temperature.
Supplementary Material (ESI) for Journal of Materials Chemistry
This journal is © The Royal Society of Chemistry 2001
2. FITC-LDH exchange rate according to NaCl concentration.
A fixed amount of FITC-LDH was added to the 1, 110-1, 110-2, 110-3, 1104
, 110-5, and 110-6 M NaCl solutions, and incubated at 37 °C for 8 hrs. After
centrifugation to remove nanoparticles, the resulting supernatant was collected to
measure fluorescence intensity. The histogram means that interlayer FITC molecules are
partially replaced by Cl- in aqueous solution and also in case of the similar to body
fluids, 110-2 or 110-3, the 23~35 % of interlayer FITC molecules can be released for 8
hours.
60
Relative fluorescence (%)
50
40
30
20
10
0
0
1
2
3
4
-Log[NaCl]
5
6
Supplementary Material (ESI) for Journal of Materials Chemistry
This journal is © The Royal Society of Chemistry 2001
Figure S-2. FITC-LDH exchange capacity at various NaCl concentrations. 1 M of
FITC-LDH was added to 1, 110-1, 110-2, 110-3, 110-4, 110-5, and 110-6 M NaCl
solution, respectively, and incubated at 37 ºC for 8hrs. After centrifugation to remove
nanoparticles, the resulting supernatant was collected to measure fluorescence intensity.
Supplementary Material (ESI) for Journal of Materials Chemistry
This journal is © The Royal Society of Chemistry 2001
3. Cytotoxicity test of Mg2Al-NO3--LDH itself.
Absorbance(arb.unit)
2.5
2.0
1.5
1.0
control
1g
10g
30g
50g
100g
300g
500g
1000g
0.5
0.0
1
2
3
4
Days
Figure S-3. Cytotoxicity experiment of LDH in HL-60 cells. Various amounts of LDH
were added to cells at final concentrations from 1  g/ml to 1000 g/ml.