BIOGRAPHICAL SKETCH
Kevin B. Temeyer
USDA, ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory
2700 Fredericksburg Road, Kerrville, Texas 78028, USA
Tel: (830) 792-0330, Fax: (830) 792-0314, Email: Kevin.Temeyer@ars.usda.gov
(a) Professional Preparation
Iowa State University, Ames, Iowa. Biology and Psychology, B.S. 1973.
Iowa State University, Ames, Iowa. Bacteriology, 37 qtr. hr. (non-degree BS equivalent) 1974.
University of Missouri, Columbia, Missouri, USA. Biolobical Sciences (Microbiology), M.A. 1977.
University of Missouri, Columbia, Missouri, USA. Biological Sciences (Cell Biology & Molecular
Genetics), Ph.D. 1982.
(b) Appointments
1989 - Present Research Molecular Biologist, USDA, ARS, Kerrville, TX
1984 - 1989
Research Microbiologist, USDA, ARS, Kerrville, TX
1982 – 1984
Research Associate (Research Microbiologist), USDA-ARS, Kerrville, TX
(c) Publications
(i) Publications Related to the Proposed Project (last 5 yrs):
Temeyer KB, Olafson PU, Brake DK, Tuckow AP, Li AY, & Pérez de León AA. 2013.
Acetylcholinesterase of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi:
gene identification, expression, and biochemical properties of recombinant proteins. Pest.
Biochem. Physiol. 106:118-123.
Swale DR, Tong F, Temeyer KB, Li AY, Lam PC-H, Totrov MM, Carlier PR, Pérez de León AA
& Bloomquist JR. 2013. Inhibitor profile of bis(n)-tacrines and N-methylcarbamates on
acetylcholinesterase from Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi.
Pest. Biochem. Physiol. 106:85-92.
Temeyer KB, Tuckow AP, Brake DK, Li AY, & Pérez de León AA. 2013. Acetylcholinesterases
of blood-feeding flies and ticks. Chemico-Biological Interactions 203:319-322.
Pérez de León AA, Rodriguez-Vivas TI, Guerrero FD, Garcia VZ, Temeyer KB, Dominguez GDI,
Li AY, Soberanes CM, Miller RJ, & Rodrigo-Cruz R. 2013. Acaricide resistance in
Rhipicephalus (Boophilus) microplus: impact on agro-biosecurity and cattle trade between
Mexico and the United States of America. Proceedings–III International Symposium of
Pesticide Resistance in Arthropods: Integrated Control of Cattle Ticks/Flies/Mitigation,
Ixtapa, Mexico, June 23-27, 2013. (Peer-reviewed Conference Proceedings)
Temeyer KB, Olafson PU, & Pruett JH. 2012. Sequence polymorphism in acetylcholinesterase
transcripts and genotyping survey of BmAChE1 in laboratory and Mexican strains of
Rhipicephalus (Boophilus) microplus. J. Med. Entomol. 49:555-562.
Li AY, Miller R, Pound JM, Guerrero F, Temeyer KB, & Pérez De León AA. 2012. The US
experience of eradicating and preventing re-infestation with cattle fever ticks. Workshop
Proceedings. 4th International Bayer Cattle Symposium, Lisbon, Portugal, June 3, 2012.
(Peer-reviewed Conference Proceedings)
Temeyer KB, Chen AC, Davey RB, Guerrero FD, Howell JM, Kammlah DM, Li AY, Lohmeyer
KH, Olafson PU, Pérez de León AA, Phillips PL, Pound JM, & Welch JB. 2012. Novel
Approaches for control of Rhipicephalus (Boophilus) microplus. Rev. Mex. Cienc. Pecu.
3(Supl 1):25-40.
Temeyer KB, Tijerina M, Davey RB, & Olafson PU. 2011. Genetic factors potentially reducing
fitness cost of organophosphate-insensitive acetylcholinesterase(s) in Rhipicephalus
(Boophilus) microplus (Acari: Ixodidae). Zoosymposia 6:260–266.
1
Olafson PU, Temeyer KB, & Pruett JH. 2011. Multiple transcripts encode glucose 6-phosphate
dehydrogenase in the southern cattle tick, Rhipicephalus (Boophilus) microplus. Exp. Appl.
Acarol. 53:147-165.
Temeyer KB, Pruett JH & Olafson PU. 2010. Baculovirus expression, biochemical
characterization and organophosphate sensitivity of rBmAChE1, rBmAChE2 and rBmAChE3,
of Rhipicephalus (Boophilus) microplus. Vet Parasitol. 172:114-121.
Pérez de León AA, Pound JM, Schuster G, Guerrero F, Davey RB, Lohmeyer KH, Hewitt DG,
Ortega AJ, Campbell TA, Racelis AE, Goolsby J, Holman P, Messenger M, Ellis D, Duhaime
R, Soliz AJ, Li AY, Phillips PL, Temeyer KB, Teel P, Wikel S, Kammlah DM, Wagner GG,
Varner KP, & Brake DK. 2010. Shift in rules of engagement for eradication of cattle fever
ticks in the United States. American Association of Veterinary Parasitologists, Proceedings.
(Peer-reviewed Conference Proceedings)
Temeyer KB, Olafson PU & Miller RJ. 2009. Genotyping mutations in BmAChE3: a survey of
organophosphate-resistant and –susceptible strains of Rhipicephalus (Boophilus) microplus.
J. Med. Entomol. 46:1355-1360.
Chen AC, He H, Temeyer KB, Jones, S, Green P & Barker SC. 2009. A survey of Rhipicephalus
microplus populations for mutations associated with pyrethroid resistance. J. Econ. Entomol.
102:373-380.
(ii) Other Significant Publications (last 2 yrs):
Tuckow AP, Temeyer KB, Olafson PU, & Pérez de León AA. 2013. Discovery of microRNAs of
the stable fly (Diptera: Muscidae) by high-throughput sequencing. J. Med. Entomol. 50:925930.
Temeyer KB, Brake DK, Tuckow AP, Li AY, & Pérez de León AA. 2013. Acetylcholinesterase
of the sand fly, Phlebotomus papatasi (Scopoli): cDNA sequence, baculovirus expression,
and biochemical properties. Parasites & Vectors 6:31.
Temeyer KB, & Chen AC. 2012. Acetylcholinesterase of Stomoxys calcitrans (L.) (Diptera:
Muscidae): cDNA sequence, baculovirus expression, and biochemical properties. Vet.
Parasitol. 184:92-95.
Temeyer KB, Brake DK & Schlechte KG. 2012. Acetylcholinesterase of Haematobia irritans
(Diptera: Muscidae): baculovirus expression, biochemical properties, and organophosphate
insensitivity of the G262A mutant. J. Med. Entomol. 49:589-594.
(d) Synergistic Activities
(i) Dr. Temeyer has presented invitational papers at the American Society for Microbiology (St.
Louis, Missouri, 1984), the Vth International Colloquium on Invertebrate Pathology and
Microbial Control (Adelaide, Australia, 1990), II International Symposium on Pesticide
Resistance (Merida, Mexico, 2009), the XIII International Congress of Acarology (Recife, Brazil,
2010), the 11th International Meeting on Cholinesterases (Kazan, Russia, 2012), and the 244th
American Chemical Society Meeting (Philadelphia, PA, 2012).
(ii) Dr. Temeyer and coworkers developed research tools, including monoclonal antibodies, dual
luciferase reporters for high throughput in vitro gene silencing in Ripicephalus microplus cell
cultures, and improved microinjection for in vivo gene silencing in R. microplus and R. annulatus.
2