Materials and Methods
DAY 1: ISOLATE DNA FROM SOY AND FOOD PRODUCTS
A. What type of soybean did YOU use?
B. What type of food did YOU use?
C. What is the total volume of Edward’s buffer used during Day 1?
D. BOIL. How long?
E. What is the purpose of boiling the samples?
F. CENTRIFUGE. How long?
G. What is the purpose of the centrifuge?
H. TRANSFER SUPERNATANT. Why not transfer the pellet?
I. CENTRIFUGE. How long?
J. At the end of Day 1 what do you have in your two tubes?
DAY 2: AMPLIFY DNA BY PCR
A. How many uL of primer/loading dye mix were added to the PCR tubes?
B. How many uL of DNA were added to the PCR tubes?
C. Two different primers are being used in this experiment. Name them.
D. The thermocycler was set at the following temperatures:
Separate the DNA Strands
Attach the Primers
Copy the DNA Strand
E. How many cycles was the thermocycler set for?
DAY 3: ANALYZE PCR PRODUCTS BY ELECTROPHORESIS
A. What goes into lane 1?
B. How many uL of liquid were added to each of the 9 lanes? What did you add to each of the
9 lanes?
1
2
3
4
5
6
7
8
9
FAKE RESULTS: DATA TABLE
 100 bp ladder
 35S 162 bp
 Tubulin 187 bp
100bp
size
marker
1
RR
35S
2
RR TEAM
Food
RR
35S Tubulin
3
4
Food
Tubulin
NRR
35S
5
6
NRR TEAM
Food
NRR
35S Tubulin
7
8
Empty Lanes
Food
Tubulin
9
10
11
12
REAL RESULTS: DATA TABLE
DRAW THE ACTUAL RESULTS AS THEY APPEAR ON YOUR GEL
 100 bp ladder
 35S 162 bp
 Tubulin 187 bp
100bp
size
marker
1
RR
35S
2
RR TEAM
Food
RR
35S Tubulin
3
4
Food
Tubulin
NRR
35S
5
6
NRR TEAM
Food
NRR
35S Tubulin
7
8
Empty Lanes
Food
Tubulin
9
10
11
12