INTRODUCTION Urbanization, which is created by the establishment of areas of high-density population, poses adverse effects on our watersheds. It has been noted that urbanization is the second leading agent of stream degradation, behind agriculture (Morgan, Cushman 2005). Also, the quality and abundance of fish species, especially native species are severely endangered. A major source of pollution is urban runoff entering the watersheds. An even bigger problem is not having adequate storm water management facilities in order to manage this problem in the correct manner (SEWRPC 2010). Runoff contains numerous dangerous chemicals, such as road salts, lawn fertilizers and automobile fluids (Wang et al 2001). The magnitude of runoff increases along with urban growth. Frequent and heavy runoff can potentially cause more flooding, eroding stream floors and banks, which further damage stream ecosystems (Wang et al 2001). The increasing flow of urban runoff leads to increased amounts of sediment being transported, reducing egg survival and creating the loss of habitat (SEWRPC 2007). The damage of water quality and stream structure has a major impact on the health of native fish species. Consequences of poor water quality are reduced survival of eggs and fish of young age and toxicity of fish species (SEWRPC 2007). Introduction or overproduction of species could take away fish habitat. Increased algae production in response to urbanization causes death of fish, due to oxygen depletion (SEWRPC 2007). Fish species and their aquatic ecosystem are becoming degraded in watersheds, due to the negative impacts of urbanization. Biological monitoring needs to be applied in order to assess watershed quality and create plans to restore its health. Biological monitoring is an essential tool in assessing the quality of a watershed and observing how urbanization plays a negative role. It can provide us with valuable information on aspects of water quality such as water chemistry, physical condition, hydrology and morphology (Anderson 2001). Judging on the quality of stream ecosystems, certain restoration measures need to be put in place. Biological monitoring helps to review what is known of aquatic ecosystems and give us the necessary information needed to restore these systems (Williams et al 1997). Biological monitoring can also be assessed to test the effectiveness of improvements that have already been made. Results of biological monitoring techniques have exposed reduced stream ecosystem quality, due to urbanization in various areas around the United States. The effects of urbanization are widely displayed in the U.S. A highly urbanized area of northeast Ohio has demonstrated negative side effects in its stream habitat due to urbanization. Fish IBI scores decreased as urban land use and population density rose (Walton et al 2007). High urbanization in areas of the Eastern Piedmont and Coastal Plain ecoregions caused low fish abundance, low fish richness, low IBI scores, and a reduced number of pollution-intolerant native species and an influx of pollution-tolerant non-native species (Morgan, Cushman 2005). Small streams in western Georgia showed increased cases of eroded fins, lesions and tumors with increased urbanization (Helms et al 2005). Also, the number of lithophilic spawners decreased as the number of herbivores increased in areas containing higher urbanization (Helms et al 2005). The adverse effects of urbanization on stream ecosystems are being exposed by biological monitoring on both a national scale, as well as a local scale. Biological monitoring of the nearby urbanized waterways of the local Menomonee River and Oak Creek showed very low biotic integrity ratings for inhabitant fish species (Anderson 2001). Most of the fish species collected in this area were tolerant to water pollution and water quality degradation (Anderson 2001). The Root River, located in Racine, Wisconsin displayed similar results. A vast majority of sample sites showed poor water quality and biotic integrity (Ortenblad et al 2003). Once again, the river was dominated by tolerant omnivores, while native and lithophilous spawners were few (Ortenblad et al 2003). The basis of this study is the biological monitoring and assessment of health and abundance of fish species located in the Kinnickinnic. The study pertains to a highly urbanized section of the river that runs through the downtown section of the Milwaukee metropolitan area. Here exist numerous sources of urbanized pollution, such as heavy traffic, shops, construction, factories, homes and apartment complexes. There are expectations of a low water quality and low health and abundance of fish species, resulting in low IBI scores. The mission of the project is to test this hypothesis, as well as create plans in order to restore the Kinnickinnic. The Milwaukee Metropolitan Sewage District has already put in place a two phase proposal to help restore the river. Phase one proposed ideas to be passed onto phase two, which is deemed the advanced planning phase (MMSD 2005). Ideas include building of bridges to go over the river, improved storm sewer improvement, erosion investigation and sedimentation investigation (MMSD 2005). Engineering improvements also need to be made. A concrete lining was placed on the bottom of the river in places in order to hopefully control flooding. The opposite took place as the lining increased the velocity of water flow and decreased the amount of vegetation (MMSD 2005). Removing the lining would be a worthy improvement. The removal of the concrete lining and widening of the river bank is currently taking place. This will slow down the river flow, and allow for increased vegetation, water quality and public safety (MMSD 2011). A pool and riffle sequence is also being implemented for fish traveling from Lake Michigan (MMSD 2011). The pools allow traveling fish to cool down and rest as they move upstream. The purpose of the research is to assess the water quality and relative health and abundance of fish species in the Kinnickinnic. After examining this data, accurate assessment will be made of the current restoration measures being taken. Proposals can then be made of what else may need to be done in order to return the river back to health. Anderson, Robert C. 2001. Southeast Wisconsin’s Menomonee River and Oak Creek Biological Evaluation 1999-2000. Wisconsin Lutheran College Biology Dept. Technical Bulletin 1: 1-29. Helms, Brian S., Jack W. Feminella and Shufen Pan. 2005. Detection of Biotic Responses to Urbanization Using Fish Assemblages From Small Streams of Western Georgia, USA. Urban Ecosystems 8:39-57. Milwaukee Metropolitan Sewage District. 2011. Kinnickinnic River Concrete Removal. Milwaukee Metropolitan Sewage District. Available: http://v3.mmsd.com/NewsDetails.aspx. (October 2012). Milwaukee Metropolitan Sewage District. 2005. Kinnickinnic River Phase 2 Watercourse Management Plan. 1:1-819. Morgan, Raymond P. and Susan F, Cushman. 2005. Urbanization Effects on Stream Fish Assemblages in Maryland, USA. Journal of the North American Benthological Society 24:643-655. Ortenblad, Angela L., David A. Bolha, and Robert C. Anderson. 2003. Sustainability Through Biological Monitoring on the Root River Racine, Wisconsin. Wisconsin Lutheran College Biology Dept. Technical Bulletin 4: 1-47. Southeastern Wisconsin Regional Planning Commission. 2010. Stream Habitat Conditions and Biological Assessment of the Kinnickinnic and Menomonee River Watersheds: 2000-2009. Memorandum Report Number 194: 1-152. Southeastern Wisconsin Regional Planning Commission. 2007. Water Quality Conditions and Sources of Pollution in the Greater Milwaukee Watersheds. Technical Report Number 39:1-141. Walton, Michael B., Mark Salling, James Wyles and Julie Wolin. 2007. Biological Integrity in Urban Streams: Toward Resolving Multiple Demensions of Urbanization. Landscape and Urban Planning 79:110-123. Wang, Lizhu, John Lyons and Paul Kanehl. 2001. Impacts of Urbanization on Stream Habitat and Fish Across Multiple Spatial Scales. Environmental Management 28(2):255-266. Williams, Jack E., Christopher A. Wood and Michael P. Dombeck, editors. 1997. Watershed Restoration: Principles and Practices. American Fisheries Society, Bethesda, Maryland.