Lake and Ocean Circulation Prof. Tina M. Niemi UMKC –Geosciences July 18-20, 2007 1 Lesson #1 - Sampling on a Lake Materials Needed: Map thermometer Van Dorn water sampler pH meter sample bottles conductivity meter marker 5-in1 Test strips GPS units Nitrate/nitrites test strips Hach dissolved oxygen kit For these two lessons, we will meet at the Gopher Lake parking lot at James A. Reed Memorial Wildlife Area in Lee’s Summit, MO. The purpose of this field trip is to investigate the stratification of a lake, the changes in water chemistry and temperature with depth, and explore the implications of these differences on water circulation and aquatic life. We will utilize the boats provided by the park and a Van Dorn-type water sampler to collect the lake water at various water depths. The water samples will be collected in bottles. Once onshore, we will use the test kits to determine various chemical properties of the water. The figure below shows the technique for sampling lake water at various depths from a boat. Source: http://esp.cr.usgs.gov/info/lacs/hohsamp.htm 2 Measuring layers in a lake Quoted from the U.S. Geological Survey (http://esp.cr.usgs.gov/info/lacs/hohsamp.htm) “Lake and ocean waters tend to form density layers based on differences in temperature, salinity and amount of suspended particles in the water. Layers of different density don't mix unless an outside force (such as a strong wind) stirs them. This kind of stratification (layering) reduces the mixing of oxygen-rich surface waters and nutrient-rich bottom water. Thus, water at different depths will have different chemistries and abilities to support plant and animal life.” “By studying water chemistry differences within the lake we learn how algal blooms, increases in precipitation and nutrient concentrations eventually affect the sediment chemistry. This information guides our interpretations of past changes.” “Water samples at specific depths are collected using van Dorn or Kemmerer-style bottles. The bottle is an open tube with spring-loaded end caps. The bottle is lowered to a particular depth and sloshed around gently (to fill the tube with water from that depth). Then a weight, called a messenger, is sent down the line holding the tube. The weight hits a spring mechanism, allowing the end caps to spring shut.” Lesson Plans: Water on the Web http://waterontheweb.org/curricula/bs/teacher.html Procedure: 1) Load sampling equipment in boats. 2) Put life vests on, and review Safety Instructions. 3) Row field team to a sampling site on the lake. 4) Take a GPS reading of your location and record it on the data sheet. 5) Prepare the van Dorn sampler, and label the first sample bottle. 6) Throw the water sampler into the water to the desired depth trigger the end to collect the sample. 7) Haul the sampler back into the boat, and transfer the water sample into the marked bottle. 8) Repeat the procedure until all five water samples have been recovered. Collect water samples from: a) Surface water b) water from one meter water depth c) water from two meter water depth d) water the bottom e) water from midway between the bottom to the surface 3 Lesson #2 - Thermocline and Water Chemistry Team Members:________________________________________________ Date: ______________ GPS Site Sampling Location:__________________ Site Description:________________________________________________ _____________________________________________________________ Weather Conditions:_____________________________________________ Water Depth Water Temperature DO, mg/L % Saturation Conductivity Nitrite/Nitrate (ppm) 5-in1 Total Chlorine (ppm) Free Chlorine (ppm) Total hardness (ppm) Total alkalinity (ppm) pH 4 DIRECTIONS FOR WATER TESTS DISSOLVED OXYGEN [PPM] 1) Pour approximately 100 ml of sample SOL-DO into a beaker. 2) Obtain the temperature of the sample using the pocket thermometer. Mark the temperature on the "Determining Percent Saturation" - Handout 3) From the Dissolved Oxygen kit remove the round bottle with the glass stopper. 4) Fill bottle with SOL-DO. 5) Incline bottle slightly and quickly insert stopper. DO NOT TRAP ANY AIR BUBBLES IN THE BOTTLE. If you do, your test will fail later and you will have to start over. 6) Open one Dissolved Oxygen 1 Reagent Powder Pillow and one Dissolved Oxygen 2 Reagent Pillow. 7) Remove stopper and add both pillows to the sample water. 8) Replace the stopper again by adding a little sample solution and using the technique in step 4. 9) Gripping the bottle and stopper firmly, carefully shake it vigorously (do not break the bottle!!) to mix the sample water and the reagents. A flocculent precipitate (floc) should begin to form. If oxygen is present, the precipitate will be a brown-orange color. o Allow the sample to stand until the floc has settled halfway in the bottle (use the marked line at the bottle), leaving the upper half of the sample clear. o Shake the sample again, and again let it stand until the upper half of the sample is clear. o Open one Dissolved Oxygen 3 Reagent Powder Pillow. Remove the stopper from the bottle and add the contents of the pillow. Carefully place the stopper back on the bottle. o Shake the sample water and reagent to mix. The floc will dissolve completely and a yellow color will develop if oxygen is present. 10) Fill the small measuring tube (full) of the prepared sample. 11) Pour the measured sample into the square mixing bottle. o Add Sodium Thiosulfate Standard Solution. ADD ONE DROP AT A TIME. Swirl the mixture around in the bottle after each drop. Count each drop as it is added. Continue adding drops until the sample changes from yellow to colorless. o Each drop used is equal to 1 ppm of dissolved oxygen. Mark the amount of drops on the "Determining Percent Saturation" - Handout o Use information on the attached "Determining Percent Saturation" – Handout to determine the amount of DO in the sample. 12) Record the value on the %Saturation Graph. 13) Pour used solution into waste water bottle!! 14) Rinse DO bottles and stopper with distilled water and return to the kit. 5 Dissolved Oxygen Percent Saturation Percent Saturation is the amount of oxygen dissolved in the water sample compared to the maximum amount that could be present at the same temperature. For example, water is said to be 100% saturated if it contains the maximum amount of oxygen at that temperature. A water sample that is 50% saturated only has half the amount of oxygen that it could potentially hold at that temperature. Sometimes water can become supersaturated with oxygen because of rapidly tumbling water. This usually lasts for a short period of time but can be harmful to fish and other aquatic organisms. DO Percent Saturation values of 80-120% are considered to be excellent and values less than 60% or over 125% are considered to be poor. Determining % Saturation - The "quick and easy" - Method For a quick and easy determination of the percent saturation value of dissolved oxygen at a given temperature, use the saturation chart below. Pair up the mg/L of dissolved oxygen you measured and the temperature of water in degrees C. Draw a straight line between temperature and mg/L of dissolved oxygen. The percent saturation is the value where the line intercepts the saturation scale. Streams with a saturation value of 90% or above are considered healthy. 6 ELECTRICAL CONDUCTIVITY [µS] 1) Remove protective cap and turn unit on by sliding the on/off switch. 2) Immerse the sensor-end of the unit into the sample. Make sure sensors are completely submerged. 3) Wait for the display to stabilize. 4) Multiply the stabilized number by 10 and record. 5) Rinse off the sensor-end of meter with distilled water. 6) Turn the unit off and replace cap. 7) Return sample to correct SOL bottle. NITRATE/NITRITE [PPM] 1) Dip a single strip into sample water for 1 second and remove. DO NOT SHAKE OFF EXCESS WATER. 2) Hold the strip level, with pad side up, for 30 seconds. 3) Compare the NITRITE test pad to the color chart on side of bottle. 4) Record the value 5) At 60 seconds, compare the NITRATE test pad to the color chart. 6) Record the value. 7) Dispose of test strip in waste basket, and replace lid on test strip bottle. 8) Dispose of used sample water in bottle labeled WASTE WATER. 1) 2) 3) 4) 5) 6) 7) 5-IN-1 TEST: TOTAL CHLORINE [PPM], FREE CHLORINE [PPM], TOTAL HARDNESS [PPM], TOTAL ALKALINITY [PPM], PH Dip one strip into the sample water for 1 second. DO NOT SHAKE OFF EXCESS WATER. Hold strip level for 30 seconds. Compare TOTAL HARDNESS, TOTAL ALKALINITY and pH pads to color chart on side of bottle. Record these values Dip strip into the sample water again and move back and forth for 30 seconds. Compare CHLORINE pad to color chart. Record data, dispose of strip, return cap to bottle and dispose of waste water into the sink. 7 Graph your data with water depth on the y-axis and other variables on the x-axis: 1. At what depth is the thermocline? 2. What layer contained the most dissolved oxygen? 3. Describe the variations in depth of other chemical properties of the lake water. 4. What is a “Dead Zone”? How do they form? 5. What is eutrophication? 8 Concept Mapping Terms: Temperature, dissolved oxygen, thermocline, nitrates, eutrophication, stratification, nutrient-rich water, oxygen-rich water, conductivity, total dissolved solids, and other terms as needed 9 Lesson #3 - Ocean Circulation 10 Exploration: Water in the ocean (Adapted from DiscoverySchool.com) http://school.discovery.com/lessonplans/programs/oceans/index.html Materials needed: Globe or map Food coloring Ice cube tray Aquarium, or large clear plastic rectangular container Hot plate Pan for boiling water Table salt Balance Thermometer 2 Petri dishes Stirring rod Examine a globe and answer the following questions. How many oceans are there and how are they connected? What is the temperature of the ocean? What temperature is at the bottom of the ocean? How does water in the ocean move? Why does ocean water circulate? 11 Activity #1: Variations in Water Temperature Preparation: The day before the experiment--Dye water with various colors (blue, red, and green work best) using food coloring and freeze in ice cube trays. 1. Fill the aquarium with warm tap water 2. Place a blue and a red ice cube at each end of the aquarium 3. Describe what happens. Activity #2: Evaporation Preparation: Place one Petri dish of tap water and a second Petri dish of salt water on the window ledge and watch it over the course of 1 to 2 weeks. Describe what you see. Activity #3: Variations in Salinity 1. Make the saltwater Measuring 17.5 g of salt on the balance Pour salt into an empty dry 500 mL cylinder Fill the cylinder to the 500 mL mark with Distilled Water Stir until salt dissolves Add food coloring 2. Fill the aquarium and a 500 mL graduated cylinder with warm tap water 3. Determine the temperature of the water 4. Slowly add the dyed water into the aquarium. 5. Describe what you observe. 12 Concept Introduction: Thermohaline Circulation “The major water masses in the deep ocean are differentiated by their temperature and salinity. These properties determine their relative densities, which in turn drive deep thermohaline circulation of the oceans. NADW = North Atlantic Deep Water. AABW = Antarctic Bottom Water. Modified from figure courtesy of Dr. Steve Hovan, Indiana University of Pennsylvania.” 1. What is density? Based on your experiments with waters of different temperatures and salinity, what makes the densest water? 2. Examine the diagram above. Where in the oceans will you find the densest water? 3. How does the atmosphere interact with the ocean? 4. What is upwelling and downwelling? Concept Mapping Density, salinity, evaporation, equator, polar, temperature, density, sodium chloride, halite, salt, thermohaline circulation, thermocline, upwelling, downwelling 13 Application: El Niño-Southern Oscillation (ENSO) Goals: 1) Describe El Niño and La Niña. Why are they important? 2) Explain how the ocean and atmosphere interact in the tropical Pacific. 3) Explain how conditions in the tropical Pacific affect climate worldwide. Procedure: 1. Examine EI Nino!-La Nina! with the slide insert pushed all the way into the device so that Neutral (normal) Conditions appears in the indentation along the bottom of the large window. (Note: If La Niña appears when fully inserted, pull the slide out, flip it to the other side, and reinsert.) 2. Look at the large window. It displays a schematic of the Pacific Ocean along the equator (greatly exaggerated in the vertical). The scene depicts the ocean surface with atmosphere above and a vertical cross-section of the ocean below. 3. Above the large window picture are the atmospheric variables shown at three longitudinal locations across the equatorial Pacific: rainfall, surface air pressure, and trade wind (direction and magnitude). 4. Below the large window picture are the ocean variables shown at two longitudinal locations across the equatorial Pacific: Surface currents, sea surface temperature, sea surface height, thermocline depth. 5. Use the Slide Card to determine the variations in Neutral, El Niño, and La Niña conditions and fill out the following charts. 6. According to the values reported in the windows, the highest sea surface temperatures (SST) during neutral conditions occur in the western tropical Pacific. Explain why this SST pattern occurs. 7. Explain how upwelling is created along the Eastern tropical Pacific. How does this affect the weather (SST, pressure, rainfall etc…) in that location? 14 Neutral (Normal) Conditions Western Pacific Atmosphere •rainfall •Air Pressure •Trade winds Eastern Pacific Ocean •Currents •SST •Sea height •thermocline El Niño Conditions Atmosphere •rainfall •Air Pressure •Trade winds Western Pacific Eastern Pacific Western Pacific Eastern Pacific Ocean •Currents •SST •Sea height •thermocline La Niña Conditions Atmosphere •rainfall •Air Pressure •Trade winds Ocean •Currents •SST •Sea height •thermocline 15 8. Changes in SST in the eastern tropical Pacific have implications across the world. Turn your EI Niño!-La Niña! over. Briefly describe the changes in the weather across the world for an El Niño episode. 9. Briefly describe the changes in the weather across the world for a La Niña episode. The following website shows current SST’s. http://www.emc.ncep.noaa.gov/research/cmb/sst_analysis/ The EI Nino!-La Nina! slide rules may be purchased through this website: http://www.ametsoc.org/amsedu/AERA/ed_mats.html Concept Mapping: El Niño, La Niña, ENSO, global weather, trade winds, upwelling, equatorial Pacific, thermocline, SST 16