Biology
Molecular biology
Department of Biology
Assoc. Prof. Dr. Ale Kucinskiene
Studentų 39, Vilnius LT-08106, room 213,. tel.: +370 5
2751813
(office),
+37069935743
(private),
ale.kucinskiene@leu.lt; ale.kucinskiene@gmail.com
English
Language of Instruction
none
Required Prerequisites
Suggested Academic Cycle Bachelor degree studies (4th year of studies)
or Year of Studies
Autumn
Semester
5
ECTS Credits
6
Contact Hours per Week
compulsory
Compulsory/ Elective
Lectures, laboratory works and individual consultations
Methods of Teaching
Individual research work (30%), 2 tests (10% + 10%), final
Form of Assessment
examination (50 %)
Title of the Study Program
Title of the Module
Faculty, Department
Instructor
Address
Course Description
The main objective of the course is to provide students with the framework for understanding
the main concepts of macromolecule structure: primary, secondary and tertiary structure of DNA
and RNA macromolecules, DNA replication in molecular level. Part of the course focuses on
transcription in prokaryotes and eukaryotes, the structure of chromatin, the ripening of RNA
molecular, RNA splicing, the importance of introns and egzons. The course gives knowledge in
biosynthesis of proteins in prokaryotes and eukaryotes.
The course also deals with the main methods of investigation: polymerase chain reaction
(principle, the use in fundamental investigations), DNA sequencing.
The course also aims at training abilities of experimental work. Much attention is paid to
learning to perform the main methods in molecular biology: DNA isolation, electrophoresis of
nucleic acids, polymerase chain reaction, restriction of lambda phage DNA.
Readings
1. Burton E. Tropp. Molecular Biology. Genes to proteins. Third edition, USA, 2008 (Internet.
adresas: http://biology.jbpub.com/molecular).
2. Geoffrey M. Cooper, Robert E. Hausman. The cell. A molecular approach. Fourth edition.
Boston University, 2007.
3. B.Lewin. Genes. Oxford, 2000 m.
4. Current protocols in molecular biology. Ed. By F.M. Ausubel. 2000 m.
5. B.Alberts et all. Molecular biology of the cell. New York, London, 1991m.
interneto tinklapiai:
www. web-books.com/MolBio/Free/Link
www. nhgi.nih.gov/educationkit/online.html.
www. genome.ad.jp./kegg/
www. average. org/pruss/nucleosome.html.