CHIRIACO, FARINELLI et al.
REGULATED LENTIVIRAL VECTORS FOR X-CGD
L. Figure S1. Schematic diagram of the lentiviral vectors used in this study.
GA RRE
BdLV.ctrl
polyA
dNGFR
PGK
GFP
WPRE
polyA
dNGFR
PGK
GFP
WPRE
Y
SD
SA
GA RRE
BdLV.miRT
miRTs
Y
SD
SA
GA RRE
PGK.gp91
PGK
gp91phox
WPRE
PGK
gp91phox
WPRE 126T 126T
MSP
GFP
WPRE
MSP
gp91phox
WPRE
MSP
GFP
WPRE 126T 126T
MSP
gp91phox
WPRE 126T 126T
SFFV
gp91phox
WPRE
Y
SD
SA
GA RRE
PGK.gp91_126T(2)
Y
SD
SA
GA RRE
MSP.GFP
Y
SD
MSP.gp91
SA
GA RRE
Y
SD
MSP.GFP.126T(2)
SA
GA RRE
Y
SD
MSP.gp91_126T(2)
SA
GA RRE
Y
SD
SFFV.gp91
SA
GA RRE
Y
SD
SA
1
CHIRIACO, FARINELLI et al.
REGULATED LENTIVIRAL VECTORS FOR X-CGD
Figure S1. Schematic diagram of the lentiviral vectors used in this study. All vectors have a
self-inactivating (SIN) configuration, with long terminal repeat (LTRs) sequences at terminal end,
the splice donor (SD) and the acceptor site (SA), the ψ encapsidation signal including the 5’
portion of the gag gene (GA), a Rev-responsive element (RRE) and a woodchuck hepatitis virus
posttranscriptional regulatory element (WPRE). From top to bottom: Phosphoglycerate kinase
promoter (PGK) driven bidirectional control (BdLV.ctrl) or miRNA regulated reporter vector
(BdLV.miRT). dNGFR: truncated low affinity nerve growth factor receptor. PGK driven, gp91 phox
expressing vectors without or with 2 tandem repeats of the miR126 target sequence (PGK.gp91,
PGK.gp91_126T(2)).Myeloid Specific Promoter- driven vectors expressing GFP or gp91phox,
with or without 2 tandem repeats of the miR-126 target sequence(MSP.GFP, MSP.gp91,
MSP.GFP.126T(2), MSP.gp91_126T(2)) Spleen focus forming virus promoter-driven vector
expressing gp91phox(SFFV.gp91).
2