Satellite Site
K McKone 2010
Lab : Micropipetting (Don’t’ let your tips touch the plastic.)
Name:______________________________________________Date:______________
**Remember 1 mL = 1,000 µL; Keep paper inside sheet protector!
**Needed: 1,100 µL of red, green, and blue water in a 1.7 mL eppies. Leave samples in the circles until
you complete the entire activity, and then clean with a paper towel.
I. Pipetting into circles. Target Practice & working on uniformity.
1) Place 5.0 µL of blue liquid inside each circle.
Total amount required: _____µL = ________mL
Pipette required: ________________
5.0 µL in each circle
2) Place 1.0 mL of red liquid inside the first circle. Pipet 2.0 µL into the other circle.
Total amount required: ______________
1.0 mL
Pipettes required: ________________
2.0 µL
3) Place 20.0 µL of green liquid inside each circle
Total amount required: _________ µL = __________mL
Pipettes required: _____________
20 µL in each circle
II. Pipetting into an eppendorf (eppie) tube.
4) Using your blue liquid, pipet the following volumes into your small eppie: 20 µL, 10 µL, & 2 µL.
*Open cap of tube before picking up micropipette!
a) Should you start with the smaller or larger volume?_________ Why?______
b) Why should you gently touch your micropipette tip to the inside wall of the reaction tube as
you expel the sample? __________________________________________________
c) If necessary, pulse-spin in the microfuge. Make sure the microfuge is balanced! Dial up a
volume of 32 µL. Withdraw the liquid from the small eppie and let the instructor see it in your tip.
_________________________________________________Teacher’s Comment