Specialist Diploma in Molecular Biotechnology (Proteomics Module)
Assessment:
Module
Proteomics
Assessment
20% Quiz
20% Practicals
60% Final exam
Lectures
8 sessions
Practicals
4 sessions
Schedule:
Mon 9th Jan
Topic 1: Introduction to Proteomics
(Lecture 1)
Objectives: Introduce the basic concepts of proteins and proteomics. A general
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outline for the proteomic process is described and briefly discussed.
Topic 2: Sample Preparation
Objectives: The various ways to extract proteins from samples are discussed.
The process of detergent lysis is given in further detail. The purification and
quantification of extracted proteins is also discussed.
th
Tues 10 Jan Topic 3: Isoelectric Focusing (IEF)
(Lecture 2)
Objectives: The theory of isoelectric focusing using CA and IPG are compared
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and discussed. The procedures to conduct IEF are further discussed.
Wed 11th Jan
(Lecture 3)
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Mon 16th Jan
(Lecture 4)
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Tue 17th Jan
(Practical 1)
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Topic 4: SDS-PAGE
Topic 5: Visualisation (Western blot)
Objectives: The basic principles and procedures of SDS-PAGE are discussed.
The visualisation of proteins on SDS-PAGE/Western blots is further discussed.
Topic 5: Visualisation (staining)
Topic 6: Comparative analysis
Topic 7: In-gel digestion
Objectives: The concepts of gel staining are discussed in relation to
comparative analysis of protein spots on 2D-gels. The various steps involved in
comparative analysis are further discussed.
Practical 1: Protein Quantification
Objectives: Total protein extracted from bacteria is quantified by BCA and/or
Bradford Assays
Wed 18th Jan
(Practical 2)
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Mon 30th Jan
(Lecture 5)
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Practical 2: SDS-PAGE
Objective: SDS-PAGE of bacterial lysate
(Quiz 1)
Topic 8: Chromatographic Techniques in Proteomics
Tue 31st Jan
(Lecture 6)
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Topic 9: Mass Spectrommetry I Techniques
Objectives: The basic principles of ionisation – MALDI-TOF and ESI-MS/MS
and mass analysis are discussed.
Wed 1st Feb
(Practical 3)
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Mon 6th Feb
(Lecture 7)
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Practical 3: IEF
Objective: IEF of bacterial lysate – also 1st dimension of 2D- gel
electrophoresis (Practical 4)
Topic 10: Mass Spectrommetry II Interpretation
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Tues 7th Feb
(Lecture 8)
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Topic 11: Quantitiation in LC-MS proteomics
Wed 8th Feb
(Practical 4)
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Practical 4: 2D-Gel electrophoresis
Objectives: 2D-gel electrophoresis of bacterial lysate
(Quiz 2)
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References:
 Check Ngee Ann library on “Proteomics”
 Check web-sites for specific queries
www.google.com)
(Use
“Google”
search
engine:
Administrative matters:
 CET student e-services (http://www.np.edu.sg/cpd/sos.htm)
-> Your survival Guidebook
Q) What is the minimum class attendance I need to attain?
: At least 80% overall class attendance
: Please submit documentary proof such as medical certificate, national service
letter, or letter from your employer (for job assignment reasons) to CPD within 48
hours from your absence from class.
Q) What happens if I am absent from the examinations?
: If you are absent from an examination due to a sudden illness, you must produce a
valid medical certificate (from a government doctor or registered medical practitioner)
within 48 hours to CPD.
: In hospitalization cases, the medical certificate must be submitted 48 hours after
you have been discharged from the hospital.
: Students who are absent from an examination without valid reasons are deemed to
have failed the module even if they have obtained 50 marks or more for the module
from the course work component alone.
: In all cases, it is important that you inform CPD or your module leader of your
absence from the examinations immediately and follow-up with the submission of
your medical certificate.
Q) What are considered valid reasons for absence during an examination?
: Hospitalization
: Medical leave
: Death of an immediate family member (ie spouse, parent, sibling, grandparent)
: Participation in approved local/overseas events
: Involvement in an accident, court case, or adverse circumstances such as floods.
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