APPLICATION
for the Taiwanese-Philippine Joint Research Project
for the years 2008-2011
under the Agreement
on scientific cooperation between the National Science Council in Taipei and the Department
of Science and Technology in Philippine
The Department of Science and Technology
---------------------------------------------------------------------------------------------------------------------
National Science Council
106 Ho-Ping East Road, Sec. 2
106 Taipei, TAIWAN
Phone: +886-2-27377959
Fax: :+48 2 27377607
e-mail: stchen@nsc.gov.tw
Title of the joint project (maximum of 10 words)
Engineering disease resistance potential in tobacco and abaca
Project subtitles
A. Pathogen tolerance conferred by H2O2-generating glucose oxidase in transgenic
tobacco
過氧化氫生成酵素glucose oxidase增進轉基因菸草抗病害能力之研究
Keywords (up to 4):
Hydrogen peroxide, Pathogen, Glucose Oxidase, Tobacco
B. Genetic engineering of abaca for abaca bunchy top virus (ABTV)
Keywords (up to 4):
Abaca, bunchy top virus, Coat protein, resistance
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Research Partners:
Institute in Philippines:
Postal code and address:
Institute in Taiwan:
Department of Life Sciences
National Chung Hsing University
Postal code and address:
Cell Physiology Laboratory
Department of Life Sciences
National Chung Hsing University
250 Kuo-Kuang road
Taichung 402, Taiwan, R.O.C.
Telephone:
Telephone: +886-4-22862511
Fax:
Fax: +886-4-22862511
e-mail:
Project coordinator (name and signature):
Dr. Ruben M. Gapasin
Director
NARC
Visayas State University
Philippines.
e-mail: chinho@dragon.nchu.edu.tw
Project coordinator (name and signature):
Lin, Chin Ho
Professor
Cell Physiology laboratory
Dept. of Life Sciences
National Chung-Hsing University
Taichung, Taiwan.
Other participants (name and position)
Other participants (name and position)
Dr. Subbiyan Maruthasalam
Post-doctoral Researcher
Department of Life Sciences
National Chung-Hsing University
Taichung, Taiwan.
Dr. Chorng Horng Lin
Assistant professor
Department of Bioresources,
Da-Yeh University
Chang-Hua
Taiwan.
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Personnel Exchange
Travel to Philippines
number of visitors
duration of each visit (days)
year 1
3
10
year 2
3
10
Year 3
3
10
number of visitors
duration of each visit (days)
Travel to Taiwan
year 1
year 2
Year 3
Project Summary:
A. Pathogen tolerance conferred by the expression of H2O2 generating
glucose oxidase controlled by PR1 promoter in transgenic tobacco
The scientific aims of the joint research, envisaged results and benefit to both sides from
this collaboration:
Plants are constantly confronted with a wide variety of potential pathogens, such as
fungi, bacteria and viruses leading to significant loss in crop productivity every year. Plants
display a wide spectrum of physiological and biochemical responses upon invasion by
pathogens. Plant-pathogen interactions elicit the various defense mechanisms in hosts, the
major one being the "programmed cell death (PCD) (Bolwell 1999). PCD potentially limit the
spread of the disease from the infection point, thus enhancing the viability of plants
(Greenberg 1997). The most common response of resistance of plants to pathogens is a rapid
production of reactive oxygen species (ROS) around the site of infection, commonly referred
as Hypersensitive Response (HR; Lamb and Dixon 1997). The death of infected areas blocks
the subsequent development of pathogen’s. Consequently, a long lasting disease resistance
develops throughout the plants, termed systemic acquired resistance (SAR) (Ryals et al. 1996).
During SAR, pathogenesis-related (PR) genes are activated triggering disease tolerance in
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plants.
Present investigation is aimed at developing transgenic tobacco plants with broad
spectrum resistance against fungal and bacterial diseases. Our strategy for achieving this goal
is to enhance the natural ability of plants to defend themselves against phytopathogens
through overproduction of ROS. The gene, glucose oxidase (from Aspergillus niger)
responsible for the production of ROS will be controlled by the pathogen inducible promoter
of the PR1 protein. This will combine the natural defense mechanisms (HR and SAR) and will
also ensure exogenous control over the expression of GO through treatment with salicylic
acid.
Present state of knowledge, significance and objectives of the joint research:
A. Related Current and Previous Work
Symptoms of plant diseases include death and destruction of host tissue, wilting,
abnormal growth and differentiation and discoloration of host tissues. Plant diseases cause
significant production losses of agricultural crops every year. Plant pathogens fall into two
broad categories; necrotrophs (those that kill plant cells before parasitizing them) and
biotrophs (those that obtain nutrients from living cells).
Plants recognize the pathogen propagule upon infection and elicit defense responses via
a vast array of signals originating from microorganisms, the environment and the plants itself.
These biotic elicitors include cell wall fragments released from fungi and bacteria, hydrolytic
enzymes of plant or pathogen origin, some peptides, glycoproteins, polyunsaturated fatty
acids, and toxic metabolites such as ROS. For survival, pathogen resistance of plants can be
expressed at any stages of the infection process. Evidences showed that defense mechanisms
induced by biotic stress are a highly coordinated system of molecular, cellular and tissuebased response in plants. Notably, the excess production of ROS is a key step to scavenge and
isolate invasive pathogens (Lamb and Dixon 1997). To date we have understood that ROS do
play diverse functions in plant defense response, described as following.
1.
Direct action
It is demonstrated that elevated levels of hydrogen peroxide inhibit the spreading of
bacterial infections and germination of spores of many fungi (Peng and Kuc 1992).
2.
Oxidative modification of cell walls
Pathogen-induced overproduction of ROS provokes fast (2-5 min after inoculation)
changes in key protein components of the cell walls such as the structure proteins p33 and
p100 rich in tyrosine and proline. Moreover, accumulation of hydrogen peroxide results in
enhanced lignifications of cell walls and alterations in their polysaccharide and pectin content
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(Bruce and West 1989; Bradley et al. 1992).
3.
Hypersensitive response (HR) induced cell death
The induction of HR is accompanied by activation of NAD(P)H oxidase and excess
production of ROS (Lamb and Dixon 1997). The over-accumulation of ROS induces rapid
death of infected areas (PCD) thus blocking further spread of the pathogens. The NAD(P)H
oxidase inhibitor diphenylene iodonium blocked the oxidative stress resulted from pathogen
attack. A similar effect was observed when tissue cultures were treated with protein-kinase
blockers straurosporin and K252 (Lamb and Dixon 1997). The inhibition of oxidative
processes substantially increases the survival of infected cells, but leads to invasion of whole
plant by the pathogen.
Wu and co-workers further confirmed the role of ROS in the pathogen tolerance (Wu et
al. 1995, 1997). In their works, the glucose oxidase (GO) gene was placed under the control of
35S promoter of figwort mosaic virus. GO is an H2O2-generating enzyme which catalyzes the
following reaction,
Glucose + O2
Gluconate + H2O2
Generally, the GO protein is secreted into the apoplast, although lower amounts of GO
could be detected in the cytoplasm fraction. Over-expression of GO led to 2-3-fold H2O2
increase in the transgenic tomato in comparison with control plants; moreover, transgenic
plants showed enhanced resistance to bacterial (Erwinia carotovora subsp carotovora) and
fungal (Phytophthora infestans, Alternaria solani) pathogens. Addition of catalase to the
nutrition medium of potato tubers neutralizes H2O2 produced from GO and reduced resistance
to pathogens.
While the above studies do indicate that H2O2 has a significant impact on the
development of pathogen tolerance, over-accumulation of H2O2 also induces programmed cell
death (PCD; Greenberg 1997). Furthermore, the constitutive expression of GO in transgenic
plants unavoidably led to abnormal growth and development of plants (Kazan et al. 1998; Lee
et al. 2002; and Kachroo et al. 2003). Kachroo et al. (2003) transformed rice plants with GO
cDNA (from A. niger – P13006; Frederick et al. 1990) controlled by two distinct promoters,
i.e. the promoter of maize constitutive ubiquitin (UB) and wound/pathogen inducible rice
phenylalanine ammonia lyase (PAL) genes. The increased amount of GO protein in UB-GO
plants showed 3-10 fold increase in the GO activity. Consequently, UB-GO transformed plants
yielded only a few seeds that showed poor viability and germination rate. By contrast, PALGO plants were phenotypically similar to the wild type and showed 2-10-fold increase in the
GO activity upon wounding. PAL-GO plants exhibited enhanced tolerance to Xanthomonas
oryzae and Magnaporthe grisea pathogens. Taken these results into consideration, pathogen
inducible expression of heterogenous GO genes seems to be a feasible way of conferring
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broad spectrum disease resistance potential and is less likely to cause developmental
abnormalities or metabolic dysfunctions in transformed plants.
B. Working hypothesis
The objective of this proposal is to develop tobacco plants transformed with a fungal GO
gene controlled by a pathogen inducible promoter. The promoter driving the GO gene will be
isolated and cloned from tobacco PR1 gene(s). PR1 gene expresses in different tissues upon
challenge with virulent and avirulent pathogens. PR1 promoter could also be activated by
exogenous application of salicylic acid (Dixon et al. 1991; Uknes et al. 1993; Agrawal et al.
2000a, b). The plants transformed with a construct consisting of promoter of salicylic aciddependent acidic PR1 and bacterial/fungal GO genes (PR1-GO lines) have the following
advantages in comparison with the above cited works:
1.
The expression of PR1-GO (and thus elevated levels of endogenous H2O2) is expected
primarily after pathogen invasion.
2.
The expression of PR1-GO can be also induced by exogenous treatment with salicylic
acid. This additional stimulation of PR1 promoter is also helpful in cases wherein the
immune responses are not triggered during pathogen infection. Moreover it is possible
that the expression of PR1-GO would exert positive effects against some abiotic stresses.
3.
PR1-GO expression is limited in time. Reactivation can be achieved during new
invasion of pathogens or treatment with salicylic acid.
We proposed here to develop two different PR1-GO tobacco transformants namely, PR1-GОe
(exocellular accumulation) and PR1-GOi (intracellular accumulation). Normally the fungal
GO protein is secreted out of the cell. Since the original 22-amino acid signal peptide
sequence from A. niger GO in the constructs used in the above cited works remained intact,
the production of the inserted genes is mainly exocellular. We suppose that PR1-GОe could
have a considerable direct effect on the phytopathogens. On the other hand, search in
databases showed the possible existence of a cytosolic GO. It is possible that the intracellular
GO to be more effective than exocellular. The construction of PR1-GOi could be of valuable
importance for evaluation of mode of action of H2O2.
Envisaged results and benefit
Innovation – The enzyme Glucose oxidase (GO) is capable of forming H2O2 by the oxidation
of glucose. Transgenic plants over-expressing GO are more resistant to various bacterial and
fungal pathogens. The production of H2O2 elicits several defence associated reactions. The
rapid oxidative burst results in rapid cell death around the pathogen infection site which is
termed as hypersensitive response (HR). In most transgenic system developed so far,
constitutive expression of GO always resulted in undesirable growth patterns. Moreover, in
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most host-pathogen interactions, the downstream events strengthening plant defence systems
are not clearly understood. The present study aims at engineering broad spectrum disease
tolerance potential in tobacco by pathogen inducible (with the use of pathogenesis-related
protein promoter) GO expression. This would definitely serves as an excellent model system
to study the hypersensitive defence responses in plants against different genera/taxa of
phytopathogens and the system could also be extended for other economically important crops
species.
Relevance - Enhancing the tolerance of crops towards broad spectrum of diseases is an
important issue on a world wide scale.
Capacity strengthening - The co-operation between the Taiwanese and Phillipine scientists,
with well experience in studying of stress responses of plants by means of molecular or
physiological approach separately, constitute a solid basis for the project execution. Of course,
exchange of research personnel will be an important feature of the joint research program.
Collaboration - Referring to the all section of the research plan, all of them will be performed
in both countries utilizing the exchange of researchers and probes. For the execution of the
scientific program the financial resources of the participating institutions will be utilized. The
first phase of the program is expected to continue over a 3-year period. The results will be
published in international journals coauthored by participants of both countries.
Research plan for the first year of collaboration:
1.
First project year – 01 July 2008 to 30 June 2009
(1)
Cloning of pathogen inducible promoter (PR1) from the 5'-flanking region of PR1
gene(s) of toabacco.
(2)
Isolation of the glucose oxidase cDNA form Aspergillus niger
(3)
Construction of pCAMBIA1301-PR1-GOi (intracellular form) and pCAMBIA1301PR1-GOe (extracellular form) vectors.
(4)
Transformation of Agrobacterium with pCAMBIA1301-PR1-GOi (intracellular form)
and pCAMBIA1301-PR1-GOe (extracellular form) vectors.
(5)
Agrobacterium-mediated transformation of tobacco with intracellular (pCAMBIA1301PR1-GOi) and exocellular (pCAMBIA1301-PR1-GOe) GO gene constructs.
Research plan for the second year of collaboration:
2.
First project year – 01 July 2009 to 30 June 2010
(1)
Molecular characterization of putative transgenic tobacco plants through Southern,
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northern and western analyses.
(2)
Isolation of fungal (Pythium aphanidermatum and Botrytis cinerea) and bacterial
(Pseudomonas syringae pv. tabaci) pathogens from diseased tobacco plants.
(3)
Evaluation of transgenic tobacco plants for disease tolerance by challenge inoculation
with fungal and bacterial pathogens.
Research plan for the third year of collaboration:
3.
First project year – 01 July 2010 to 30 June 2011
(1) Estimation of exo- and endogenous H2O2 generation in transgenic plants expressing GO
and wild plants upon challenge with the pathogens.
(2) Study of downstream defence (pathogenesis-related protein accumulation, phytoalexin
production, accumulation of phenols and cell wall structural proteins, antioxidant defense
enzymes, etc) responses in transgenic plants harbouring GO gene elicited by the H2O2
activity during plant-pathogen interaction.
(3) Study of interaction between exogenous salicylic acid application and H2O2 production in
transgenic plants.
References:
Agrawal, G.K. Rakwal, R., and Jwa, N. (2000a) A novel rice (Oryza sativa L.) acidic gene
highly responsive to cut phytohormones, and protein phosphatase inhibitors. Biochem.
Biophys. Communic. 274: 157-165.
Agrawal, G.K., Rakwal, R., and Jwa, N. (2000b), Rice (Oryza sativa L.) OsPR1b gene is
phytohormonally regulated in close interaction with light signals. Biochem. Biophys.
Communic. 278: 290-298.
Bolwell, G.P. (1999) Role of active oxygen species and NO in plant defense responses. Curr.
Opin. Plant Biol. 2: 287-294.
Bradley, D.J., Kjellbom, P., and Lamb, C.J. (1992) Elicitor and wound-induced oxidative
cross-linking of a proline-rich plant cell wall protein: a novel rapid defense response, Cell
70: 21-30.
Bruce, R.J. and West, C.A. (1989) Elicitation of lignin biosynthesis and isoperoxidase activity
by pectic fragments in suspension cultures of castor bean. Plant Physiol. 91: 889-897.
Dixon, D., Cutt J., and Klessig, D. (1991) Differential targeting of the tobacco PR1
pathogenesis related proteins to the extracellular space and vacuoles of crystal idioblasts.
8
EMBO J. 10: 1317-1324.
Frederick, K.R., Tung, J., Emerick, R.S., Masiartz, F.R., Chamberlan, S.H., Vasavada, A.,
Rosenberg, S., Chakraborty, S., Schopter, L.M., and Massey, V. (1990) Glucose oxidase
from Aspergillus niger. Cloning gene sequence, secretion from Saccharomyces cerevisiae
and kinetic analysis of a yeast derived enzyme. J. Biol. Chem. 265: 3793-3802.
Greenberg, J.T. (1997) Programmed cell death in plant-pathogen interaction. Ann. Rev. Plant
Physiol. Plant Mol. Biol. 48: 525-545.
Kachroo, A., He, Z., Patkar, R., Zhu, Q., Zhong, J., Li, D., Ronald, P., Lamb, C., and Chattoo,
B. (2003) Induction of H2O2 in transgenic rice leads to cell death and enhanced resistance
to both bacterial and fungal pathogens. Transgenic Res. 12: 577-586.
Kazan, K., Murray, F.R., Goulter, K.C., Llewellyn, D.J., and Manners J.M. (1998) Induction
of cell death in transgenic plants expressing a fungal glucose oxidase. Mol. Plant Microbe
Interact. 11: 555-562.
Lamb, C. and Dixon, R.A. (1997) The Oxidative burst in plant disease resistance. Ann. Rev.
Plant Physiol. Plant Mol. Biol. 48: 251-275.
Lee, Y.H., Yoon, I.S., Suh, S.C., and Ki, H.I. (2002) Enhanced disease resistance in transgenic
cabbage and tobacco expressing a glucose oxidase from Aspergillus niger. Plant Cell Rep.
20: 857-863.
Peng, M. and Kuc, J. (1992) Peroxidase-generated hydrogen peroxide as a source of
antifungal activity in vitro and on tobacco leaf disks. Phytopathology 82: 696-699.
Ryals, J.A., Neuenschwander, U.H., Willits, M.G., Molina, A., Steiner, H.Y., and Hunt, M.D.
(1996) Systemic acquired resistance. Plant Cell 8: 1809-1819.
Uknes, S., Dincher, S., Friedrich, L., Negrotto, D., Williams, S., Thompson-Taylor, H., Potter,
S., Ward, E., and Ryals, J. (1993) Regulation of pathogen-related protein-1a gene expression
in tobacco. Plant Cell 5: 159-169.
Wu, G.S., Short, B.J., Lawrence, E.B., Leon, J., Fitzsimmons, K.C., Levine, E.B., Raskin, I.,
and Shah, D.M. (1997) Activation of host defense mechanisms by elevated production of
H2O2 in transgenic plants. Plant Physiol. 115: 427-435.
Wu, G.S., Short, B.J., Lawrence, E.B., Levine, E.B., Fitzsimmons, K.C., and Shah, D.M.
(1995) Disease resistance conferred by expression of a gene encoding H2O2-generating
glucose oxidase in transgenic potato plants. Plant Cell 7: 1357-1368.
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Taiwan Project Leaders and PIs
PRIMARY INFORMATION
Name and SURNAME:
Chin-Ho Lin
e-mail:
chinho@dragon.nchu.edu.tw
Phone:
+886-4-22862511
Fax:
+886-4-22862511
Address for correspondence:
Department of Life Science, National Chung-Hsing University,
250, Kuo Kuang Road, Taichung, Taiwan
EDUCATION AND EMPLOYMENT
1963-1967, B.S. Department of Botany, National Chung Hsing University, R.O.C.
1971-1973, M.S. Botany, University of California, Davis, U.S.A.
1974-1976, Ph.D. Plant Physiology, University of California, Davis, U.S.A.
PROFESSIONAL EXPERIENCE
1969-1971
Teaching Assistant, Department of Botany, National Chung Hsing University
1972-1976
Research Associate, Department of Botany, University of California, Davis
1976-1983
Associate Professor, Institute of Botany, National Chung Hsing University
1983
Recipient of “Outstanding Research professor” by Ministry of Education,
Central government.
1983-2002
Professor, Department of Botany, National Chung Hsing University
1993
Visiting Professor, Division of Biological Sciences, University of California,
Davis
1993-95
Recipient of “Outstanding Research award” by National Science Council,
Central Government
2001-2003 President, Botanical Society of the Republic of China.
2003/2004
Research Project Coordinator, Biological Division National Science Council,
Central Government.
2002-till date Professor, Department of Life Sciences, National Chung Hsing University
PROFESSIONAL MEMBERSHIP
1. American Society of Plant Biologists
2. Botanical Society of the Republic of China
RESEARCH INTERESTS
1. The scientific activity is concentrated on plant response to different environmental
stresses, in particular; chilling, flooding and oxidation stresses. The research cover
physiological, biochemical and molecular approaches of plant response as well as
measure alleviate stresses.
2. Flowering physiology of tropical fruits.
3. Recovery of plant growth regulator contaminated soil of orchard.
4. Delaying of natural flowering in pineapple.
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TEACHING SUBJECT
Plant Physiology, Cell Biology, Plant Hormone, Biological Microtechniques
PATTERN HOLDING
Plant natural Vernalization detecting and logging device
PUBLICATIONS
1
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1. 秉訓, 林金和 2000. Plastochron Index 在植物生理學上之意義. 科學農業.
48(9,10):249-253.
2. Chung-Ta Liao and Chin-Ho Lin , 2001. Physiological responses of plants to flooding
stress (invited review) Proceedings of National Science Council Part B: Life science. Vol.
25(3) 148-157.
3. Chin Ho Lin, Bing Shiunn Chen. Chin Wen Yu and Shih Wen, Chiang. 2001. A WaterBased Triphenyltetrazolium Chloride Method for the Evaluation of Green Plant Tissue
Viability. Phytochemical Analysis.Vol.12. 211-213.(SCI)
4. Yu-Ting Wu and Chin-Ho Lin. 2001 Analysis of cytokinin activity of commercial
aqueous seaweed extract. Gartenbauwissenschaft, 65 (4).S. 170-173.
5. Yu-Mei Hsu, Menq-Jiau Tseng, and Chin-Ho Lin. 2001. Alteration of Polypeptides
Synthesis in Flooded Wax-apple (Syzygium samarangense Merr.et Perry) Roots.
Gartenbauwissenschaft. 66(44).s.182-187.(SCI)
6. 許玉妹, 曾夢蛟, 林金和. 2002.
淹水逆境下蓮霧根部酒精脫氫酵素同功異構酵素之純化與生化特性之探討.
台灣農業化學與食品科學會誌. 40(1):19-2
7. Lai,Rong-Mao,Shao-Pin Yo,Yu-Mei Hsu,and Chin-Ho Lin.2001.Hydrogen Peroxide
Reduced Chilling Induced Fruit Abscission in Syzygium Samarangense Merr.et
Perry._Gartenbauwissenschaft. 66(6).S.289-292(SCI)
8. Chih-Wen Yu, Terence M. Murphy, Wei-Wen Sung and Chin-Ho Lin. 2002. H2O2
treatment induces glutathione accumulation and chilling tolerance in mung bean. Funct.
Plant Biol., 29, 1081-1087. (SCI)
9. Jing-Yueh Huang and Chin-Ho Lin. 2003. Cold Water Treatment Promotes Ethylene
Production and Dwarfing in Tomato seedlings. Plant Physiology and Biochemistry 41,
283-288. (SCI)
10. Chih-Wen Yu, Terence M. Murphy and Chin-Ho Lin. 2003. Hydrogen peroxide-induced
chilling tolerance in mung beans mediated through ABA-independent glutathione
accumulation. Funct. Plant Biol. 30. 955-963.(SCI)
11. 楊苑欣, 林金和, 陳福旗. 2003. Thidiazuron及2,4D或Plicloram促進火鶴花試管苗葉片之培養再生不定芽.中國園藝. 49(4). 375-382
12. Yo, S-P, and C.-H Lin. 2004. Qualitative and Quantitative Composition of the Flavour
Components of Taiwan Calamondin and Philippine Calamansi Fruit. Europ. J. Hort. Sci.
69(3). 245-252.(SCI)
13. Jung-Kang Jin, Douglas O. Adams, Yeong Ko, Chin-Wen Yu, and Chin-Ho Lin. 2004.
Aviglycine and Propargylglycine inhibit conidial germination and mycelial growth of
Fusarium oxysporum f. sp. luffae. Mycopathologia.158:369-375, 2004. (SCI)
14. Ching-San Kuan, Chin-Wen Yu, Mei-Li Lin, Hsin-tszu Hsu, Duane P. Bartholomew and
Chin-Ho Lin. 2005. Foliar application of aviglycine reduces natural flowering in
pineapple. Hortscience. 40(1):123-126, 2005.(SCI)
15. Sht-Hsien Hung, Chih-Wen Yu, Chin Ho Lin. Hydrogen peroxide functions as a stress
signal in plants. Bot. Bull. Acad. Sin. 46:1-10, 2005.
16. Jung-Kang Jin, Hui-Ling Chen, Chih-Wen Yu, and Chin Ho Lin. Establishment of a
plastochron index for growth assessment of Phalaenopsis. Can. J. Bot. 83: 1–7 (2005)
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17. Hazak, J.C., D.O. Adams, C.H. Lin* and B-H Ro. 2005. The phenolic components of
grape berries in relation to wine composition. Acta Horticulturae 689:189-196 (2005)
18. Maruthasalam, S., Lin, C.H.*, Loganathan, M., Ko, Y., Lien, W.C., Chen, C.C. and
Chuang, S.H. 2006. Gene sequence submission in NCBI database Accession #
DQ830987. Isolation and characterization of 1-aminocyclopropane-1-carboxylate
deaminase gene from a plant growth promoting Pseudomonas strain.
19. Wang, R.H., Hsu, Y.M., Bartholomew, D.P., Maruthasalam, S. and Lin, C.H.* 2007.
Delaying Natural Flowering in Pineapple through Foliar Application of Aviglycine, an
Inhibitor of Ethylene biosynthesis. HortScience 42(5):1188-1191. (SCI).
20. Ko, Y., Yao, K.S., Chen, C.Y and Lin, C.H.* 2007. First report of Gray Mold disease of
Sponge Gourd (Luffa cylindrica) caused by Botrytis cinerea in Taiwan. Plant Dis. 91
(9):1199. Disease Notes (SCI).
21. Ko, Y., Yao, K.S., Chen, C.Y and Lin, C.H.* 2007. First report of Gray Leaf Spot of
Mango (Mangifera indica) Caused by Pestalotiopsis mangiferae in Taiwan. Plant Dis.
91(12):1684 - Disease Notes (SCI)
22. Ko, Y., Yao, K.S., Chen, C.Y., Liu, C.W., Maruthasalam, S. and Lin, C.H.* 2008. First
Report of Gummosis Disease of Plum (Prunus salicina) Caused by Botryosphaeria sp. in
Taiwan. Plant Dis. 92 (3): 483. Disease Notes (SCI).
23. Ko, Y., Chen, C.Y., Yao, K.S., Liu, C.W., Maruthasalam, S. and Lin, C.H.* 2008. First
report of fruit rot of strawberry (Fragaria chiloensis var. ananassa) caused by Alternaria
tenuissima in Taiwan. Plant Dis. (Accepted for publication) Disease Notes (SCI).
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PRIMARY INFORMATION
Name and SURNAME:
Subbiyan Maruthasalam
e-mail:
asmaruthu@hotmail.com
Phone:
+886-4-22862511
Fax:
+886-4-22862511
Address for correspondence:
Department of Life Science, National Chung-Hsing University,
250, Kuo Kuang Road, Taichung, Taiwan, R.O.C.
EDUCATION AND EMPLOYMENT
1992-1996: B.S. Agriculture, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu,
India.
1997-1999: M.S. Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil
Nadu, India.
1999-2003: Ph.D. Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil
Nadu, India.
PROFESSIONAL EXPERIENCE
2003-2005 Research Associate, Center for Plant Molecular Biology and Biotechnology,
Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
2005- till date Post Doctoral Researcher, Department of Life Sciences, National Chung
Hsing University, Taichung, Taiwan, R.O.C.
PROFESSIONAL MEMBERSHIP
1. Indian Phytopathological Society
2. Indian Society of Plant Pathologists
HONORS
1997-1999 - Junior Research Fellow, Novartis India Limited, Mumbai, India.
2000-2002 - Senior Research fellowship, Department of Biotechnology, New Delhi, India.
2002
- Prof. M. J. Narasimhan Academic Merit Award commendation certificate
RESEARCH INTERESTS
1. Genetic transformation of crop plants with disease resistance/defence genes
2. Delaying natural flowering in pineapple through RNA interference (RNAi)
3. Gene isolation from bacteria and plants
PUBLICATIONS
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1. Kamalakannan, A., Shanmugam, V. and Maruthasalam, S. 2004. Evaluation of maize
genotypes for resistance to Sorghum Downy Mildew (SDM) caused by
Peronosclerospora sorghi (Weston and Uppal) C.G. Shaw. Pl. Dis. Res. 19(1): 60-63.
2. Maruthasalam, S., Sabitha Doraiswamy, Shanmugam, V. and Kamalakannan, A. 2005.
Evaluation of inoculation techniques to screen maize genotypes against Sorghum Downy
Mildew. Ann. Pl. Prot. Sci. 13(1): 233-234.
3. Kalaiarasan, P., Maruthasalam, S., Sivakumar, M., Sudhakar, D. and Balasubramanian,
P. 2005. An efficient regeneration protocol for tomato (Lycopersicon esculentum L.) cv.
PKM1. Full paper published in the proceedings of the National symposium on
Biotechnological interventions for improvement of horticultural crops: Issues and
strategies, held at Thrissur, Kerala, India, January 10-12, 2005.
4. Kumar, K.K., Maruthasalam, S., Loganathan, M., Sudhakar, D. and Balasubramanian,
P. 2005. An improved Agrobacterium-mediated transformation protocol for recalcitrant
elite indica rice cultivars. Plant Mol. Biol. Reptr. 23 (1): 67-73.
5. Kalpana, K., Maruthasalam, S., Rajesh, T., Poovannan, K., Kumar, K.K., Kokiladevi,
E., Raja, J.A.J., Sudhakar, D., Velazhahan, R., Samiyappan, R. and Balasubramanian, P.
2006. Engineering sheath blight resistance in elite indica rice cultivars using genes
encoding defense proteins. Plant Sci. 170: 203-215.
6. Maruthasalam, S., Kalpana, K., Kumar, K.K., Loganathan, M., Poovannan, K., Raja,
J.A.J., Kokiladevi, E., Samiyappan, R., Sudhakar, D. and P. Balasubramanian. 2007.
Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight
and bacterial blight. Plant Cell Rep. 26: 791-804.
7. Maruthasalam, S., Lin, C.H.*, Loganathan, M., Ko, Y., Lien, W.C., Chen, C.C. and
Chuang, S.H. 2006. Isolation and characterization of 1-aminocyclopropane-1carboxylate deaminase gene from a plant growth promoting Pseudomonas strain. Gene
sequence submission in NCBI database Accession # DQ830987.
8. Wang, R.H., Hsu, Y.M., Bartholomew, D.P., Maruthasalam, S. and Lin, C.H.* 2007.
Delaying Natural Flowering in Pineapple through Foliar Application of Aviglycine, an
Inhibitor of Ethylene biosynthesis. HortScience 42(5):1188-1191. (SCI).
9. Ko, Y., Yao, K.S., Chen, C.Y., Liu, C.W., Maruthasalam, S. and Lin, C.H.* 2008. First
Report of Gummosis Disease of Plum (Prunus salicina) Caused by Botryosphaeria sp. in
Taiwan. Plant Dis. 92 (3): 483. Disease Notes (SCI).
10. Ko, Y., Chen, C.Y., Yao, K.S., Liu, C.W., Maruthasalam, S. and Lin, C.H.* 2008. First
report of fruit rot of strawberry (Fragaria chiloensis var. ananassa) caused by Alternaria
tenuissima in Taiwan. Plant Dis. (Accepted) Disease Notes (SCI).
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PRIMARY INFORMATION
Name and SURNAME:
Chorng-Horng Lin
e-mail:
clin@mail.dyu.edu.tw
Phone:
+886-4-8511888 ext 6214
Fax:
+886-4-8511326
Address for correspondence:
Dept. of Bioresources, Da-Yeh University, 112 Shan-Jiau Rd.
Da-Tsuen, Changhua, 515, Taiwan.
EDUCATION AND EMPLOYMENT
1985-1988: BS. Dept. of Botany, National Chung Hsing University, Taichung, Taiwan.
1988-1990: MS, Dept. of Botany, National Chung Hsing University, Taichung, Taiwan.
1993-1998: Ph.D, Dept. of Molecular Biology, Vanderbilt University, Nashville, TN, USA.
Apr. 1998- Mar. 1999
May, 1999- Jul. 2001
Aug. 2001-Jul. 2004
Aug. 2004- to date
PROFESSIONAL EXPERIENCE
Postdoctoral fellow in the laboratory of Dr. Leslie Leinwand, Dept. of
Molecular, Cellular and Developmental Biology, University of
Colorado at Boulder, USA
Postdoctoral fellow in the laboratory of Dr. JiannShiun Lai, Institute of Biomedical Sciences, Academia Sinica,
Taiwan
Research Associate, National Museum of Marine Biology and
Aquarium. Taiwan.
Assistant professor, Dept. of Bioresources, DaYeh University, Taiwan.
HONORS
Chosen for the oral presentation in the first RNA society meeting (1996) in Madison,
Wisconsin, USA
Graduate research excellence award in Molecular Biology, Vanderbilt University, USA (1998)
RESEARCH INTERESTS
1. Bacterial diversity in paclobutrazol applied agricultural soils.
2. Circulating laddered DNA fragments in acute stroke patients.
3. Characterization of 5’-flanking region of the sea anemone ATP ribosylation factor 1 and
beta-actin genes
PUBLICATIONS
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1. Lin, C.H., and Lin, C.H. (1992) Physiological adaptation of waxapple to waterlogging.
Plant, Cell and Environment. 15: 321-328.
2. Lin, C.H., and Patton, J.G. (1995) Regulation of alternative 3’ splice site
selection by constitutive splicing factors. RNA 1: 234-245.
3. Perez, I.,* Lin, C,-H.,* McAfee, J.G., and Patton, J.G. (1997) Mutation of PTB
binding sites causes misregulation of alternative 3’ splice site selection in vivo. RNA 3: 764778.
4. Dye, B.T., Buvoli, M., Mayer, S.A., Lin, C.H., and Patton, J.G. (1998) Enhancer elements
-tropomyosin exon 2. RNA 4:15231536.
5. Chen, M.-C., Cheng, Y.-M., Wang, L.-H., Lin, C.-H., Huang, X.-Y., Liu, M.-C., Sung, P.-J.,
and Fang, L.-S. (2004) Cloning and characterization of the first cnidarian ADP-ribosylation
factor, and its involvement in the Aiptasia-Symbiodinum endosymbiosis. Marine
Biotechnology. 6(2):138.
6. Shiue, Y-L, Wang, L-H, Chao, T-Y, Lin, C-H, and Tsai, C-L (2004) EST-based
identification of genes expressed in the hypothalamus of adult tilapia, Oreochromis
mossambicus. Biochem. Biophys. Res. Commun. 316(2): 523-527.
7. Kuo, Jimmy, Chen, M.-C., Lin, C.-H., and Fang, L.-S., (2004) Comparative gene expression
in the symbiotic and aposymbiotic Aiptasia pulchella by expressed sequence tag analysis.
Biochem. Biophys. Res. Commun. 318:176-186.
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Philippine research project and Collaborator details
Project Summary
B. Genetic engineering of abaca for abaca bunchy top virus (ABTV)
The scientific aims of the joint research, envisaged results and benefit to both sides from
this collaboration:
Present state of knowledge, significance and objectives of the joint research:
A. Related Current and Previous Work
B. Working hypothesis
Envisaged results and benefit
Innovation
Relevance
Capacity strengthening
Collaboration
Research plan for the first year of collaboration:
1.
First project year – 01 July 2008 to 30 June 2009
Research plan for the second year of collaboration:
2.
First project year – 01 July 2009 to 30 June 2010
Research plan for the third year of collaboration:
3.
First project year – 01 July 2010 to 30 June 2011
References:
Philippine Project Leaders and PIs
PRIMARY INFORMATION
Name and SURNAME:
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8
e-mail:
Phone:
Fax:
Address for correspondence:
EDUCATION AND EMPLOYMENT
PROFESSIONAL EXPERIENCE
PROFESSIONAL MEMBERSHIP
RESEARCH INTERESTS
TEACHING SUBJECT
PATTERN HOLDING
PUBLICATIONS
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