Faculty of Engineering
List of Experiment / Activity
Department:
S/N
Last updated: 29 Feb 2008
Lab Supervisor
Location/Project
Work Experiment / Activities (Routine /
Non-Routine)
Priority
Target Date for
Assessment
Faculty of Engineering
Activity Sequence List - Activity or Experiment-Based Risk Assessment
Experiment / Activity Steps
Department:
No.
BiE
Experiment / Activity
Bacteria culture of Bacillus PS3 and
Halobacterium Saliinarum
Location:
E3A 7 floor
Laboratory:
Nanobioanalytics
Workable Sequence/Task
Preparation of culture media
Autoclave sterilisation of media and labware
Bacteria culture in shaking incubator
Use of spectrophotometer to track culture progress
Centrifuge of bacteria cells/membranes
Chemical/detergent treatment and assays of cell membranes and protein
Last updated: 29 Feb 2008
Faculty of Engineering
Activity or Experiment-Based Risk Assessment Form
Department:
BIE
Name of Experiment/Activity:
Bacteria culture (Bacillus PS3 and Halobacterium Salinarum)
Location:
Nanobioanalytics Lab
Name of Person in-charge:
Tan Wee Jin
Last Review Date:
1
Task
Preparation of
Hazards
Spillage
media
2. Risk Evaluation & Control
Skin/Eye contact,
Existing Risk
Control
(if any)
Lab coat, covered
inhalation
hand/foot/eyewear,
Possible
Consequences
S
L
R
1
2
2
2
1
2
2
1
2
, take precautions
based on MSDS
2
Autoclave
High temperature
Skin/eye contact,
Lab coat, covered
sterilisation and
and pressure
inhalation, spillage,
hand/foot/eyewear,
burns
follow standard
drying of
media/labware
eqpt operating
instructions
3
Dr Dieter Wilhelm Trau
Next Review Date:
1. Hazard Identification
No.
Name of PI:
BSL 1 Bacteria
Moving parts
Skin/eye contact,
Lab coat,
culture in
inhalation, spillage,
hand/eye/footwear
incubator/shaker
glass breakage,
protection, follow
eqpt malfunction
standard operating
instructions,
perform std
decontamination
with bleach/ 70%
ethanol in case of
Last updated: 29 Feb 2008
Additional /
New Risk
Control
S
L
R
Action By
Deadline
Faculty of Engineering
spillage
Use of
Spillage
Spectrophotomet
Contamination,
Use of protective
eqpt malfunction.
eqpt, Follow std
er
1
1
2
3
1
3
operating and
decontamination
instructions
Use of centrifuge
Spillage, high
Contamination,
Use of protective
Check Motor in
speed moving parts
eqpt malfunction
hand/foot/eyewear
case of
and eqpt, balance
suspected
all bottles before
damage, e.g. in
centrifuge, check
case rotor falls
safe operating
onto ground
speeds, follow
operating
instructions
Chemical/deterge
Spillage
Skin/eye/inhalation
Use protective
nt treatment and
contact. Work area
wear, proper
assays of
contamination
decontamination
membranes/
procedures, follow
protein
chemical MSDS
1
3
3
Use smaller
volumes
safety procedures
Conducted by: (Name, designation)
Approved by: (Name, designation)
Signature:
Signature:
Date:
Date:
Last updated: 29 Feb 2008
Risk Assessment Matrix
Likelihood
Severity
Minor
Moderate
Major
Remote (1)
Occasional (2)
Frequent (3)
1
2
3
2
4
6
3
6
9
(1)
(2)
(3)
Severity Categories
Level
Human (Impact to
Physical Being)
Biological Impact
Environmental
Damage
Property Damage (S$)
(1) Minor
No Injury or light injury
requiring only first aid
treatment (MC < 4 days
MC)
Any injury/ill health
leading to ≥ 4 days MC or
≥ 1 day hospitalisation or
leads to temporary
disability
May not cause human disease, if does, the disease
is unlikely to spread to the community and there is
usually effective prophylaxis or treatment available;
Reversible
Up to $5,000
Can cause severe human disease, not ordinarily
spread by casual contact from one individual to
another; it may spread to the community, but there
is usually effective prophylaxis or treatment
available
Reversible but takes
years
$5,001 to $50,000
Fatality, permanent
Disability or life
threatening disease
Can cause lethal human disease, may be readily
transmitted from one individual to another, or from
animal to human or vice-versa directly or indirectly,
or casual contact, it may spread to the community;
usually no effective prophylaxis or treatment
available
Irreversible
More than $50,000
(2) Moderate
(3) Major
Likelihood Categories
Level
(1) Remote
(2) Possible
(3) Frequent
Events Frequency
Undesired event which may occur but unlikely, once in 5 years
Undesired event which is probable, once in a year
Undesired event which probably occur in most circumstances, once or more in a month
Risk Level and Acceptability Criteria
Risk Score
Risk Level
Acceptability
of Risk
<3
Low Risk
Acceptable
Medium Risk
Moderately
Acceptable
3–4
Last updated: 29 Feb 2008
Recommended Actions
No additional risk control measures required. To continue to monitor to ensure risk do not escalate to higher level.
Acceptable to carry out the work activity; however, task need to be reviewed to bring risk level to As Low As Reasonably
Practicable.
Interim control measures such as administrative controls can be implemented. Supervisory oversight required.
Faculty of Engineering
Job must not be carried out until risk level is brought to at least medium risk level.
>4
High Risk
Not Acceptable
Risk controls should not be overly dependant on personal protective equipment. Controls measures should focus on
Elimination, substitution and engineering controls.
Immediate Management intervention required to ensure risk being brought down to at least medium level before work can
be commenced.
Last updated: 29 Feb 2008