UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Methylmagnesium Bromide, 3.0M Diethyl Ether
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☒Hazardous Chemical
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Purpose
Methylmagnesium bromide is a highly flammable compound that reacts violently with water. Contact with
water releases flammable gases which may ignite spontaneously. It is considered a pyrophoric chemical. It
may also form explosive peroxides. It is harmful if ingested or absorbed through the skin and may be harmful
if inhaled. It causes burns by all exposure routes. Methylmagnesium bromide is a Grignard reagent that can be
used as a strong base and in nucleophilic subsitutions.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 75-16-1; diethyl ether: 60-29-7
Class: Flammable, pyrophoric, water reactive, harmful by ingestion, corrosive, peroxide former
Molecular Formula: CH3BrMg in C4H10O
Form (physical state): Liquid
Color: Grey-brown
Boiling point: N/A
Potential Hazards/Toxicity
Methylmagnesium Bromide, 3.0M Diethyl Ether
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Methylmagnesium bromide is a highly flammable liquid and vapor that is both light and air-sensitive. It reacts
violently with water. Contact with water releases flammable gases which may ignite spontaneously. It is a
pyrophoric liquid. Handle and store under inert gas and protect from moisture. May form explosive
peroxides. It is harmful if ingested or absorbed through the skin and may be harmful if inhaled. It is an
aspiration hazard if ingested. Causes burns by all exposure routes. Material is extremely destructive to the
tissue of the mucous membranes and upper respiratory tract. Causes skin and eye burns with severe damage.
Vapors may cause drowsiness or dizziness. Symptoms of exposure include burning sensation, coughing,
wheezing, laryngitis, shortness of breath, headache, nausea, vomiting, chemical pneumonitis, and
inflammation and edema of the bronchi. Diethyl ether has a permissible exposure limit (PEL) of 1200 mg/m3.
Engineering Controls
Methylmagnesium bromide should be used in a glove box or in a closed system in a certified chemical fume
hood.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Handle with gloves. Fluorinated rubber gloves are recommended. Nitrile gloves may also be used.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with methylmagnesium bromide, 3.0 M diethyl ether.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved, tight-fitting glasses/goggles. Face shields are recommended.
Skin and Body Protection
Flame-resistant lab coat preferably made of antistatic material, long pants, closed-toe shoes.
Hygiene Measures
Avoid contact with skin, eyes, and clothing. Wash hands before breaks and after handling.
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Consult a physician.
In case of skin contact
Methylmagnesium Bromide, 3.0M Diethyl Ether
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Immediately flush with plenty of water for at least 15 minutes while removing contaminated clothing. Consult
a physician.
In case of eye contact
Flush eyes with plenty of water for at least 15 minutes lifting upper and lower eyelids and removing contact
lenses. Consult a physician. Continue rinsing eyes during transport to hospital.
If swallowed
Do not induce vomiting. Never give anything by mouth to an unconscious person. If victim is conscious and
alert, rinse mouth with water and give 2-4 cupfuls of milk or water. Consult a physician.
Special Handling and Storage Requirements
Precautions for safe handling: Avoid contact with skin, eyes, and clothing. Avoid inhalation and ingestion.
Use spark-proof tools and explosion-proof equipment. Provide adequate ventilation. Keep away from heat
and other sources of ignition- No smoking. Prevent build-up of electrostatic charge.
Conditions for safe storage: Keep container tightly closed in a cool, dry, and well-ventilated area. Opened
containers must be carefully resealed and kept upright to prevent leakage. Never allow contact with water.
Handle and store under inert gas. Store protected from moisture and sunlight. Store in flammables area. Store
away from combustible materials. Light, moisture, and air-sensitive. Avoid oxidizing agents, acids, alcohols,
and oxygen.
Always date peroxide forming chemicals with the date of receipt and the date of opening the chemical. Please
completely use or dispose of the chemical within one year of the opening/receipt date.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Methylmagnesium Bromide, 3.0M Diethyl Ether
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
Methylmagnesium Bromide, 3.0M Diethyl Ether
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Methylmagnesium Bromide, 3.0M Diethyl Ether
5
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)