BIOLOGY 1 STANDARD 4.0 LESSON 4.9 PRINT OUT THIS LESSON BEFORE CONTINUING IN ORDER TO FOLLOW THE DIRECTIONS AND COMPLETE THE ASSIGNMENT. KEEP THESE PAGES IN YOUR NOTEBOOK Genetics and Biotechnology The student will investigate the concepts of genetics and heredity, different methods of reproduction, patterns of inheritance, and genetic disorders; as well as, explore and evaluate the DNA technologies from both a scientific and ethical perspective. Lesson 5 - Investigate genetic engineering and DNA fingerprint technology. Use the link “Create a DNA fingerprint” to explore this exciting new technology. Don’t forget to print out a copy of the questions before you start. DNA Fingerprinting Create a DNA fingerprint Students will learn the steps of DNA fingerprinting by creating a fingerprint in a virtual lab. They will use this fingerprint to solve a virtual crime. The virtual lab is interactive and goes through the step-by-step process of DNA fingerprinting Introduction: 1. DNA is unique for everyone. The only exception is if a person has what? 2. What are DNA fingerprints used for? Part 1 “It Takes a Lickin” 3. What “crime” was committed? 4. What bodily fluid was removed from the “crime scene” to get DNA? Part 2 “DNA Fingerprinting at the NOVA Lab” 5. What does a restriction enzyme do? 6. What is agarose gel? 7. What is electrophoresis? 8. Smaller fragments of DNA move ____________ than longer strands? 9. Why do you need to place a nylon membrane over the gel? 10. Probes attach themselves to __________ 11. Which chemical in your “virtual lab” is radioactive? 12. Sketch your DNA fingerprint. Use the following link to learn more about DNA technology, you should go through three activities (out lined below) Use the next arrow at the bottom of the screen to continue though the lesson. Hands On DNA Fingerprinting 1. The "Hey! You're Me!" button takes you to a story about two people who meet their identical twin they never knew and turn to DNA Fingerprinting to find out if they really are related. If you don't know much about DNA Fingerprinting start here! 2. The "Tutorial" button takes you to the Tutorial, which allows you to interactively learn about DNA Fingerprinting. This section is detailed, and assumes you know some terminology to start with. 3. The "Try It Out" button takes you to the "Try It Out" section of the Tutorial, where you can gain some hands-on experience with some of the steps involved with DNA Fingerprinting Go to the following link and answer the practice problems regarding DNA Technology. Bio-technology practice problems Instructions: The following problems have multiple choice answers. Correct answers are reinforced with a brief explanation. Incorrect answers are linked to tutorials to help solve the problem. 1. 2. 3. 4. 5. 6. Southern hybridization technique Determining Paternity Father's Profile Rape investigation VNTR: hypervariable regions Probability