000/15
NUS Institutional Animal Care and Use Committee
Date revised: Jun 2015
Form expiry date: 31 Dec 2015
Procedure D
Use of Animal/Human Tissue/Fluids and Hazardous Agents
All research projects are required to undergo a safety and health risk assessment prior to
commencement. Principal Investigators (PI) are to ensure they have obtained the approval of OSHE
and comply with all NUS safety & health procedures prior to starting the project. (Risk assessment
SOP, information and templates are available from OSHE at http://www.nus.edu.sg/osh/index.html)
1. Administration of hazardous agents, human/animal tissue and/or fluid to animals:
Biological materials must be tested for specific pathogens from a CM approved
diagnostic laboratory prior to use in animals. Please see CM Biological Material testing
SOP #605.02 for information for testing.
(a)
Chemicals (including biochemicals and recombinant proteins):
Species:
Number of Animals:
Please provide the following information:
Name of agent
(b)
Location of
use (Bldg,
Room No)
Concentration
(amount per
unit volume,
e.g. mmol / L)
Volume
used
Possible complications / side effects /
hazards
to humans
Infectious Agents (including bacteria, viruses, parasites, prions):
Species:
Number of Animals:
(i) Please provide the following information if they are to be introduced into
animals:
Location of use (Bldg, Room
No)
Name of agent
Animal
containment
(ABSL)
(ii) Is the agent attenuated?
Yes
No. Please explain:
(iii) Principal Investigators must undertake the following :
ensure that the appropriate safe work practices are followed for the assigned
Biosafety levels.
Procedure D
1
000/15
NUS Institutional Animal Care and Use Committee
ensure that the all personnel working in CM ABSL-2 facilities to enrol and complete
the Faculty Orientation.
(c)
Human blood, body fluids, normal or neoplastic tissues/cells:
Please attach IRB approval
Species:
Number of Animals:
(i) Please provide the following information if they are to be introduced to
animals:
Name of cells, tissues or fluids
Location of use (Bldg, Room No)
(ii) Pathogen screening of samples.
1. Please note that the protocol can only be approved by IACUC after the sample has
been screened or exempted from screening.
2. Please submit Appendix B of CM Biological Material testing SOP #605.02 to CM
for screening or exemption (Contact CM at 65166410 or lacacca@nus.edu.sg).
3. The following samples may be exemptedfrom screening:

Non-murine derived biological that have NOT been passaged through rodent or
exposed to murine products.

Commercially obtained biologicals for which the vendor can supply
satisfactory negative screening results.
(iii) Will Universal Precautions be followed when handling human blood,
body fluids, or tissues?
Yes.
No. Please explain:
(iv) Principal Investigators are responsible for the safety of their personnel
and will have to undertake the following :
ensure that all personnel working with human blood, body fluids or tissues have
been vaccinated for Hepatitis B.
ensure that all personnel working with human blood, body fluids or tissues obtain
blood born pathogen training. Training records will be accessible. Training
certificate, if applicable, is attached or will be submitted.
not to start the project before approval from Institutional Review Board (IRB) is
obtained.
not to start the project before the biologics have been screened for murine
pathogens, or exempted. (refer to SOP # 605.02)
(d)
Animal blood, body fluids, normal or neoplastic tissues/cells:
Species:
Number of Animals:
(i) Please provide the following information if they are to be introduced into
animals:
Procedure D
2
000/15
NUS Institutional Animal Care and Use Committee
Name of the species cells, tissues or
fluids
Location of use (Bldg, Room No)
(ii) Pathogen screening of samples.
1. Please note that the protocol can only be approved by IACUC after the sample has
been screened or exempted from screening.
2. Please submit Appendix B of CM Biological Material testing SOP #605.02 to CM
for screening or exemption (Contact CM at 65166410 or lacacca@nus.edu.sg).
3. The following samples may be exempted:

If the murine biological material is derived from donor animals located in the
same facility and room as the recipients (or approved room within the same
facility with the same health status).

Non-murine derived biological that have NOT been passaged through rodent or
exposed to murine products.

Commercially obtained biologicals for which the vendor can supply
satisfactory negative screening results.
(iii)Principal Investigators are responsible for ensuring that all materials to
be used are free from murine pathogens and will have to undertake the
following :
not to start the project before the cells, tissues or fluids have been screened for
murine pathogens or exempted. (refer to SOP # 605.02)
(e)
Recombinant DNA (including transgenic organisms):
Please read the recent multi-agency joint circular on Biosafety Guidelines for
Research, Release and Importation of Genetically Modified Organisms and ensure
compliance with the guidelines when using genetically modified organisms.
Species:
Number of Animals:
(i) Please provide the following information if they are to be introduced into
animals:
Name of agent
Location of use (Bldg, Room No)
(ii) Principal Investigators must undertake the following :
ensure that the transgenic organisms or the animals receiving recombinant DNA
or transgenic organisms are properly contained in the housing and use locations
to prevent escape and that they are not released to the environment.
(f)
Anaesthetic Gases:
Species:
Number of Animals:
(Anaesthetics provided by CM and used in CM facilities are not required to be listed below)
Name of agent
Procedure D
Location of use (Bldg, Room No)
3
000/15
NUS Institutional Animal Care and Use Committee
(g)
Radiation /Radioactive materials:
Species:
Number of Animals:
Radioisotopes or radiation sources
Location of use (Bldg, Room No)
2. Handling of hazardous materials (including animals, bedding, cages and wastes,
and ambient water for aquatic animals), animal and/or human tissue/fluid:
(a) Who will be responsible for handling the above materials and have they been
trained?
List all personnel handling hazard
Describe the safety training received on handling
hazardous materials
(b) Following the administration of the hazardous materials, who will be
responsible for the following?
Researchers
listed in 2 (a)
Tasks
CM
staff
Department
husbandry
staff
1. Husbandry
2. Health check
3. Management of contaminated cage/bedding or
contaminated ambient water of aquatic animals
3. Storage of hazardous materials:
Name of agent
Storage
location
(Bldg, Room
No)
Physical state
Maximum
quantity stored
(activity, volume,
titre or
concentration)
Source
4. Disposal of hazardous materials:
Who will perform the disposal?
Tasks
Researchers
listed in 2 (a)
1. Removal and bagging
2. Autoclave (where applicable)
3. Incineration
Procedure D
4
CM
staff
Department
husbandry
staff
Disposal
contractor
NUS Institutional Animal Care and Use Committee
000/15
5. Declaration:
I affirm the accuracy of the information provided in this form.
I will abide by the applicable declarations in 1(b)(iii), 1(c)(iv), 1(d)(iii) and 1(e)(ii) above.
I will not begin work on the project prior to the approvals from all the relevant authorities
(IACUC, IRB and OSHE).
I will ensure that all personnel working with hazardous agents, animal and/or human
cells/tissue/fluids, or recombinant DNA obtain the appropriate training in advance of starting
work on project. Training records will be accessible. Training certificate, if applicable, is
attached or will be submitted.
I will ensure that all personnel handling the hazardous materials, including husbandry staff
in departmental facilities and CM, are informed of the risks and instructed on the methods of
safe handling.
I will ensure that accident, injuries or other adverse events are reported immediately to the
CM and OSHE.
-- End of Procedure D –
Procedure D
5