SUPPORTING INFORMATION Diversification of subgenus Calathus (Coleoptera: Carabidae) in the Mediterranean region – glacial refugia and taxon pulses Carlos Ruiz, Bjarte H. Jordal and José Serrano Journal of Biogeography Appendix S2 Supplementary figures on subgenus Calathus phylogeny. Figure S1 Sampling localities. Locality codes are indicated in Table S1 of Appendix S1. Figure S2 The 50% majority consensus tree of subgenus Calathus resulting from the Bayesian analysis of the cox1–cox2 sequence. Numbers above nodes show posterior probability ≥ 0.90. Bootstrap support values are shown below nodes. Circle numbers are discussed in the text. MED: Mediterranean region; NA: North Africa; IB: Iberian Peninsula; IT:Italian Peninsula; BA: Balkan Peninsula; AN: Anatolian Peninsula; CA: Caucasus, LV: Levant region; EU: Central and North Europe; -S: Southern distribution; -C: Central distribution; -N: Northern distribution; -W: Western distribution; -E: Eastern distribution. Figure S3 The 50% majority consensus tree of the subgenus Calathus resulting from the Bayesian analysis of the EF1α sequence. Numbers above nodes show posterior probability ≥ 0.90. Bootstrap support values are shown below nodes. Circle numbers and squared letters refer to particular clades which are discussed in the text. Figure S4 The 50% majority consensus tree of the subgenus Calathus resulting from the Bayesian analysis of the 28S sequence aligned with MAFFT. Numbers above nodes show posterior probability ≥ 0.90. Bootstrap support values are shown below nodes. Circle numbers and squared letters refer to particular clades which are discussed in the text. Figure S5 The 50% majority consensus tree of Iberian Calathus (clade 9) resulting from the Bayesian analysis of mitochondrial (cox1–cox2) and nuclear (EF1a and 28S) markers. Numbers above and below nodes as in Fig. 3. Background colour shows the approximate distribution of Iberian species belonging to clade 9. Arrows indicate inferred introgression events. Figure S6 Graphic representation of the 95% confidence intervals of time estimates for 8 sister species in the subgenus Calathus under different calibration analyses. Analysis A was based on 9 calibration points, analysis B on previously reported rates. The shaded area represents Pleistocene glacial cycles.