Principal Investigator/Program Director (Last, First, Middle):
Mao, Jianren
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
John Joseph Anthony Marota
Assistant Professor, Harvard Medical School
Assistant Anesthetist, Mass. General Hospital
eRA COMMONS USER NAME
JJMAROTA
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
DEGREE
(if applicable)
INSTITUTION AND LOCATION
Haverford College Haverford, PA
Penn. State Univ. College of Med., Hershey, PA
Penn. State Univ. College of Med., Hershey, PA
B.S.
Ph.D.
M.D.
YEAR(s)
1978
1983
1985
FIELD OF STUDY
Chemistry
Biological Chemistry
Medicine
A. Positions and Honors
Positions and Employment
1978 – 1979 Summer Research Assistant, Dept. Chemistry, Haverford College
1979
Research Assistant, Dept. Biological Chemistry, Penn State College of Medicine
1980
Research Assistant, Dept. Biological Chemistry, Penn State College of Medicine
1980 – 1983 Graduate Research Assistant, Dept. Biological Chemistry, Penn State College of Medicine
1985 – 1986 Internship, Transitional Program, York Hospital, York, PA
1986 – 1989 Resident in Anesthesia, Milton S. Hershey Medical Center, Hershey, PA
1989 – 2000 Instructor, Department of Anaesthesia, Harvard Medical School
1989 – 1990 Clinical and Research Fellow (Neuroanesthesia) Dept. of Anesthesia, Massachusetts General
Hospital
1989 – 1991 Guest Worker, Laboratory of Molecular Neurobiology, Addiction Research Center, National
Institute of Drug Abuse
1991 – 1999 Assistant in Anesthesia, Department of Anesthesia, Massachusetts General Hospital
1999 Assistant Anesthetist, Department of Anesthesia and Critical Care, Massachusetts General
Hospital
2000 Assistant Professor, Department of Anesthesia, Harvard Medical School
Honors
1978 Departmental Honors, Chemistry, Haverford College
1978 College Honors, for research, Haverford College
B. Selected peer-reviewed publications (in chronological order).
1. Marota JJ, and Shiman R. Stoichiometric reduction of phenylalanine hydroxylase by its cofactor: a
requirement for enzymatic activity. Biochemistry. 1984; 23: 1303-1311.
2. Reaction of rat liver phenylalanine hydroxylase with fatty acid hydroperoxides. Characterization and
mechanism. J Biol Chem. 1988; 263:5646-55.
3. Marota JJ, Crosby G, and Uhl GR. Selective effects of pentobarbital and halothane on c-fos and jun-B
gene expression in rat brain. Anesthesiology. 1992; 77: 365-371.
4. Goto T, Marota JJ, and Crosby G. Nitrous oxide induces preemptive analgesia in the rat that is
antogonized by halothane. Anesthesiology. 1994; 80: 409-416.
5. Goto T, Marota JJ, and Crosby G. Pentobarbitone, but not propofol, produces pre-emptive analgesia in the
rat formalin model. Br J Anesth. 1994; 72: 662-667.
6. Kearse LA Jr, Marota JJ. The role of the electroencephalogram in assessing memory function under
general anesthesia. J Clin Anesth. 1994; 6(6):457-61.
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Biographical Sketch Format Page
Principal Investigator/Program Director (Last, First, Middle):
Mao, Jianren
7. Crosby G, Marota JJ, Goto T, and Uhl GR. Subarachnoid morphine reduces stimulation-induced but not
basal expression of preproenkephalin in rat spinal cord. Anesthesiology. 1994; 81:1270-1276.
8. Crosby G, Marota JJ, and Huang PL. Intact nociception-induced neuroplasticity in transgenic mice deficient
in neuronal nitric oxide synthase. Neuroscience. 1995; 69:1013-1017.
9. Goto T, Marota JJ, and Crosby G. Volatile anesthetics antagonize nitrous oxide and morphine-induced
analgesia in the rat. Br J Anesth. 1996; 76:702-706.
10. Zaharchuk G, Bogdanov AA, Jr., Marota JJ, Shimizu-Sasamata M, Weisskoff RM, Kwong KK, Jenkins BG,
Weissleder R, and Rosen BR. Continuous assessment of perfusion by tagging including volume and water
extraction (CAPTIVE): a steady-state contrast agent technique for measuring blood flow, relative blood
volume fraction, and the water xtraction fraction. Magn Reson Med. 1998; 40:666-678.
11. Mandeville JB, Marota JJ, Kosofsky BE, Keltner JR, Weissleder R, Rosen BR, and Weisskoff RM. Dynamic
functional imaging of relative cerebral blood volume during rat forepaw stimulation. Magn Reson Med.
1998; 39:615-624.
12. Wilkins AS, Marota JJ, Tabit E, and Kosofsky BE. Transplacental cocaine exposure. 3: Mechanisms
underlying altered brain development. Neurotoxicol Teratol. 1998; 20: 239-249.
13. Marota JJ, AyataC, Moskowitz MA, Weisskoff RM, Rosen BR, and Mandeville JB. 1999. Investigation of
the early response to rat forepaw stimulation. Magn Reson Med. 41:247-252.
14. Mandeville JB, Marota JJ, Ayata C, Zaharchuk G, Moskowitz MA, Rosen BR, and Weisskoff RM. Evidence
of a cerebrovascular postarteriole windkessel with delayed compliance. J Cereb Blood Flow Metab. 1999;
19: 679-689.
15. Zaharchuk G, Mandeville JB, Bogdanov AA, Jr., Weissleder R, Rosen BR, Marota JJ, Iadecola C, and Kim
SG. Cerebrovascular dynamics of autoregulation and hypoperfusion. An MRI Study of CBF and changes in
total and microvascular cerebral blood volume during hemorrhagic hypotension. Stroke. 1999; 30:21972204; discussion 2204 – 2195.
16. Mandeville JB, Marota JJ, Ayata C, Moskowitz MA, Weisskoff RM, and Rosen BR. MRI Measurment of the
temporal evolution of relative CMRO2 during rat forepaw stimulation. Magn Reson Med. 1999; 42:944-951.
17. Mandeville JB, and Marota JJ. Vascular filters of functional MRI: spatial localization using BOLD and CBV
contrast. Magn Reson Med. 1999; 42:591-598.
18. Marota JJ, Mandeville JB, Weisskoff RM, Moskowitz MA, Rosen BR, Kosofsky BE. Cocaine activation
discriminates dopaminergic projections by temporal reponse: an fMRI study in rat. NeuroImage 2000;
11:12-23.
19. Boas DA, Gaudette T, Strangman G, Cheng X, Marota JJA, Mandeville JB. The accuracy of near infrared
sectroscopy and imaging during focal changes in cerebral hemodynamics. NeuroImage. 2001; 13:76-90.
20. Mandeville JB, Jenkins BG, Kosofsky BE, Moskowitz MA, Rosen BR, Marota, JJA. Regional Sensitivity and
coupling of BOLD and CBV changes during stimulation of Rat Brain. Magn. Reson. Med. 2001; 45:443-7.
21. Packard SD, Mandeville JB, Ichikawa T, Ikeda K, Terada K, Niloff S, Chiocca EA, Rosen BR, Marota JJ.
Functional response of tumor vasculature to PaCO2: determination of total and microvascular blood
volume by MRI. Neoplasia. 2003; 5(4):330-8.
22. Mandeville JB, Jenkins BG, Chen YI, Choi J, Kim Y, Belen D, Liu C, Kosofsky BE, Marota JJ. Exogenous
contrast agent improves sensitivity of gradient-echo fMRI at 9.4 Tesla. Magn. Reson. Med. 2004; 52:127281.
23. Liu CH, Greve DN, Dai G, Marota JJ, Mandeville JB. Remifentanil administration reveals biphasic phMRI
temporal response in rat consistent with dynamic receptor regulation. Neuroimage 2007; 34(3):1042-53.
24. Jones RCW, Ren JQ, Chou T, Russ A, Marota JJA, Mandeville JB, Weisskoff R, Moskowitz MA, Rosen
BR, Kosofsky BR. Dopamintergic influences on cocaine-induced activation in rat brain: A 2-DG and fMRI
study. J Comparative Neurology, Revisions requested. *
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Principal Investigator/Program Director (Last, First, Middle):
Mao, Jianren
C. Research Support
Ongoing Research Support
5 R21 DA021773-02 Marota (PI)
09/30/06-07/31/08
NIH/NIDA
Effects of Cocaine Self-administration: fMRI of Awake Non-human Primates
Role on Project: Principal Investigator
The goal of this project is to develop the technology necessary to perform functional MRI in awake behaving
primates in order to image self-administration of cocaine.
Completed Research Support
5 KO8 DA14665 A 04 Marota (PI) 08/25/02-07/31/07
NIH/NIDA
fMRI Characterization of Acute Tolerance to Cocaine
Role on Project: Principal Investigator
The goal of this project is to use magnetic resonance technology to elucidate the mechanisms underlying the
development of acute tolerance to cocaine.
5R01 EB00357-03 Mandeville (PI) 7/1/00-6/30/03
NIH
Role on Project: Investigator
MRI Measurement of Relative CMR02: Validation using PET
Research supported by this grant will 1. Develop and validate MR methods for quantitative imaging of the
relative regional cerebral metabolic rate of oxygen utilization (rCMRO2) under different physiological conditions
in animal models and in human volunteers, and 2) validate the relationship between changes in CMRO2 and
hemodynamics in the setting of focal brain activation modulated by neuronal activity.
5 P01 DA 09467-10 Rosen (PI) 9/30/94-6/30/05
NIH/NIDA
Functional Brain Mapping of Cocaine Action (Project 3)
Role On Project: Investigator
The major goals of this project are to understand the circuitry in humans that underlies cocaine action based
on functional magnetic resonance imaging of human subjects and animal models. As a clinical anesthesiologist
and experienced researcher in rodent studies, Dr. Marota plays a key role in the success of the rodent studies.
NCRR P41 RR14075 04 Rosen (PI)
9/28/99-8/31/05
NIH
Role on Project: Investigator
Center for Functional Imaging Technologies
Regional Resource for functional brain imaging.
Dr. Marota is a clinical anesthesiologist and an experienced researcher in rodent studies. His participation is
essential to the success of the rodent studies.
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