Ampicillin
Kanamycin
Agar
Hazardous Substances Policy - Assessment
LB Broth
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Rober H. Insall
Assessor
Frank Zimmermann
Assessment Number*
2040
Date of Assessment
04.03.02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
8th Floor labs
List names where possible
Laura Machesky, Tom Millard, Juana Magdalena, Jacqui Woodings, Isabel
Olazabal, Sophie Launay, Robert Insall, Sophie Launay, Celine Costa, Kam Sethi
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Electroporation of E. coli
AMOUNT**
Attach copies of data sheet(s)
HAZARD
Ampicillin
50 mg/ml
Harmful
Kanamycin
50 mg/ml
Toxic
Agar
LB Broth
1.5 g/l
20 g/l
Non hazardous
but avoid
inhalation of
dust as a
precaution.
E. coli
R42:/43 May cause
sensitization by
inhalation or skin contact.
R61: May cause harm to
the unborn child.
see biological coshh
Signalling to Cell Motility
DNA
5
RISK PHRASES
3 µg
Non hazardous.
INTENDED USE
Give brief details and attach protocol/instructions
To transform E. coli by electroporation.
HAZDAT NO***
BIOSCIENCESNO***
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Ampicillin: May cause sensitization by inhalation and skin contact.
Kanamycin: May cause congenital malformations in the foetus.
Risk of burns from incorrect use of electroporator.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The level of risk is not acceptable and control measures are necessary.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Keep away from heat. Keep away from sources of ignition. Empty containers pose a fire risk, evaporate the
residue in a fume hood. Do not ingest. Do not breathe dust.
Avoid contact with skin. Wear suitable protective clothing. If ingested, seek medical advice immediately and
show the container or the label. Keep away from incompatibles such as oxidizing agents, moisture.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Only staff trained in the use of the electroporator will be allowed to perform this task.
10
MONITORING
Performance of control measures,
Check fume hood is working properly before use.
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Waste plates shall be double bagged in autoclave bags and autoclaved at 121˚C for 50min.
Place the waste into a yellow bag and seal for disposal. Kill bacteria with Virkon according to the manufacturers
instructions.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
November 2007 or when more information about Kanamycin becomes available.
13
EMERGENCY ACTION
TO CONTROL HAZARDS
To stabilise situation eg spread absorbent on liquid spill; eliminate sources of ignition, etc.
Ventilate the area. Wear a facemask. Extinguish any naked flames.
Damp down solids with a water spray to reduce aerosols.
Use a scoop to put the material into a yellow bag for incineration. Finish cleaning by spreading water on the contaminated surface and mop up with
absorbent material. Dispose into yellow bags for incineration.
TO PROTECT PERSONNEL
Evacuation, protection for personnel involved in clean-up, Special First Aid
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately.
Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If large
quantities of this material are swallowed, call a physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband.
In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Cold
water may be used. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water
may be used. Get medical attention.
TO RENDER SITE OF EMERGENCY SAFE
Clean-up/decontamination
Use a scoop to put the material into a yellow bag for incineration. Finish cleaning by spreading water on the contaminated surface and mop up with
absorbent material. Dispose into yellow bags for incineration.
R. H. Insall
CONTACT L.Machesky
42507
PHONE 42504
10.10.00
*
Prefix T is used for Teaching Assessment Number.
**
List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
***
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.