Translational Research Preconference
Targeting Tumour Cell Signaling and Microenvironment
Facilitator: Robert Bristow
Location: Centre Ballroom
Wednesday, September 10, 2008
DRAFT 08JUN08
Objectives
Upon completion of this symposium, attendees should be able to:
 Describe the role of hypoxia in the prediction of radiotherapy response
 Describe novel strategies to image and target the tumour microenvironment to sensitize
tumours to radiotherapy
 Understand the role of abnormal intracellular signaling in radiotherapy resistance and novel
molecular-targeting agents which may sensitize tumour to radiotherapy.
7:00-8:00
Registration and Breakfast
8:00-8:15
Introduction and Overview
Robert Bristow
8:15-9:00
Educational Seminar
Current Status of Hypoxia and Radiotherapy
Anthony Fyles
9:00-9:50
Scientific Session 1- Molecular and Functional Imaging
Moderators:
9:00-9:12
1
ULTRASOUND MICROBUBBLE-POTENTIATED ENHANCEMENT OF
TUMOUR RESPONSE TO RADIATION: PRELIMINARY RESULTS
Justin Lee1, Raffi Karshafian2, Behzad Banihashemi3, Amanda Caissie3, Anoja
Giles2, Peter Burns2, Gregory Czarnota1
Odette Cancer Centre, Toronto, ON1; Sunnybrook Health Sciences, Toronto,
ON2; University of Toronto, Toronto, ON3;
9:12-9:24
2
DCE-MRI PREDICTING FOR RESIDUAL NECK DISEASE: INTEGRATING
PRE-TREATMENT TUMOUR PERFUSION IN THE OVARLL MANAGEMENT
OF NECK METASTASIS
Harry Quon, University of Pennsylvania, Philadelphia, PA
9:24-9:36
3
A PILOT STUDY TO ASSESS INTRA-TREATMENT FDG-PET PARAMETERS
THAT PREDICT FOR LOCOREGIONAL CONTROL IN ADVANCED HEAD AND
NECK CANCER TREATED WITH CHEMORADIATION
Ian Poon, Curtis Caldwell, Judith Balogh, Robert MacKenzie, Danny Enepekides,
Kevin Higgins Odette Cancer Centre, Toronto, ON
9:36-9:48
4
A PILOT PROSPECTIVE STUDY USING FDG PET CT IN THE ASSESSMENT
OF METABOLIC AND ANATOMIC RESPONSE BEFORE, DURING, AND
AFTER RADIOTHERAPY IN LUNG CANCER
Gerald Lim, Alex Sun, Barbara Wysocka, Douglas Vines, Marc Freeman, Andrea
Bezjak, John Cho, Anthony Brade, Andrew Hope, Stephen Breen
Princess Margaret Hospital, Toronto, ON
9:50-10:10
Break
10:10-11:00
Scientific Session 2 - Molecular Targeting of Radiotherapy
Moderators:
10:10-10:22
5
COMBINATION OF IONIZING RADIATION AND NOVEL BINARY TARGETED
MOLECULE POTENTIATES CYTOTOXIC THERAPY
Mitra Heravi, Atta Goudarzi, Zakaria Rachid, Bertrand Jean-Claude, Thierry
Muanza
McGill University, Montreal, QC
10:22-10:34
6
LOVASTATIN SENSITIZES NON-SMALL CELL LUNG CANCER (NSCLC)
CELLS TO RADIATION; POSSIBLE INVOLVEMENT OF THE AKT SIGNALING
PATHWAY
Ayesha Tsakiridis1, Ciaqiong Liu1, Evangelia Tsiani2, Gurmit Singh1, Jim
Wright1
Juravinski Cancer Centre, Hamilton, ON1; Department of Community Health
Science, St. Catharines, ON
10:34-10:46
7
RADIATION INDUCED B1–INTEGRIN EXPRESSION AND RESPONSE TO
THERAPY IN HER-2 OVEREXPRESSING BREAST CANCER CELL LINES
Bassam Abdulkarim, John Thoms, Siham Sabri, David Lesniak, David Murray
Cross Cancer Institute, Edmonton, AB
10:46-10:58
8
THE INVASIVENESS OF BREAST CANCER CELL IS ENHANCED BY
RADIATION
Benoit Paquette, Rosalie Lemay, Claude Pépin, Luc Trembaly, Martin Lepage
Université de Sherbrooke, Sherbrooke, QC
11:00-12:00
Keynote Speaker
Drug Penetration and Solid Tumours
Andrew Minchinton
12:00-1:30
Lunch and Poster Viewing and Discussion
Moderators:
110
INHIBITION OF ATM AND DNA-PK LEADS TO RADIOSENSITIZATION UNDER
HYPOXIA
Robert Bristow1, Carla Coackley1 Mecthild Krause2, Mark Albertella3, Andrew
Slade3, Graeme Smith3
Princess Margaret Hospital, Toronto, ON1; Medical Faculty Carl Gustav Carus,
University of Technology Dresden, Dresden, Germany2; KUDOS
Pharmaceuticals, Cambridge, United Kingdom3
111
SOLVATED ELECTRONS, NOT SO HARMLESS AFTER ALL: GENERATION
OF INTERSTRAND CROSS-LINKS UNDER HYPOXIC CONDITIONS
Marie-Eve Dextraze1, Sylvain Cecchini2, François Bergeron3, Sonia Girouard1,
J. Richard Wagner1, Darel J. Hunting1
Université de Sherbrooke, Sherbrooke, QC; National Institutes of Health,
Bethesda, MD2; Commissariat à l'Énergie Atomique, Grenoble, France3
112
THE IMPACT OF HYPOXIA ON RADIOTHERAPY (RT) AND CHEMORADIOTHERAPY (CRT) RESPONSE IN LOCALLY ADVANCED CERVICAL
CANCER: A DEFINITIVE CARO TRANSLATIONAL RESEARCH GROUP
MULTICENTRE STUDY
Corinne Doll1, Anthony Fyles2, Christina Aquino-Parsons3, Peter Craighead1,
Michael Milosevic2, Robert Bristow2, Richard Hill2, Susan Lees-Miller3, Peggy
Olive4, Malcolm Hayes4, Michael Eliasziw5, Melania Pintilie2, Anthony
Magliocco1
Tom Baker Cancer Centre, Calgary, AB1; Princess Margaret Hospital, Toronto,
ON2; British Columbia Cancer Agency, Vancouver, BC3; Dept of Biochemistry
and Molecular Biology, Calgary, AB3, University of British Columbia, Vancouver,
BC4; Dept of Pathology, Vancouver, BC4; Dept of Community Health Science,
Calgary, AB5
113
ROLE OF INTERCELLULAR ADHESION MOLECULE-1 IN CENTRAL
NERVOUS SYSTEM RADIATION INJURY
Shun Wong, Yu-qing Li, Kailiang Wu
Sunnybrook Health Sciences Centre, Toronto, ON
114
PET CT THRESHOLDS FOR TARGET DEFINITION IN NON-SMALL CELL
LUNG CANCER: HOW CLOSE ARE WE TO PATHOLOGY?
Kailiang Wu1, Yee C. Ung1, Jennifer Hornby2, Marc Freeman2, David Hwang3,
Ming Tsao3, Max Dahele1, Gail Darling3, Donna Maziak4, Romeo Tirona1,
Kathy Mah1, C. Shun Wong1
Odette Cancer Centre, Sunnybrook Health Sciences Centre, Toronto, ON1;
University Health Network, Toronto, ON2; Princess Margaret Hospital, Toronto,
ON3; Ottawa Regional Cancer Centre, Ottawa, ON4
115
INVESTIGATING RESVERATROL IN COMBINATION WITH RADIATION IN
PROSTATE CANCER
Ayesha Tsakiridis, Ciaqiong Liu, Jim Wright, Ian Dayes, Himu Lukka, Gurmit
Singh, Theodoros Tsakiridis
Juravinski Cancer Centre, Hamilton, ON
116
PET/CT FOR RADIOTHERAPY TREATMENT PLANNING IN PATIENTS WITH
SOFT TISSUE SARCOMAS
Irene Karam, Slobodan Devic, Marc Hickeson, David Roberge, Roberge
Turcotte, Carolyn R. Freeman
McGill University Health Centre, Montreal, QC
117
TARGET DELINEATION USING PET CT IN NON-SMALL CELL LUNG
CANCER (NSCLC): HOW MUCH DIFFERENCE BETWEEN
AUTOCONTOURING AND MANUAL CONTOURING?
Kailiang Wu1, Yee C. Ung2, David Hwang3, Ming Tsao3, Gail Darling4, Donna
Maziak5, Romeo Tirona2, Kathy Mah2, C.Shun Wong2
Cancer Hospital of Fudan Univeraity, Shanghai, Shanghai, China1; Odette
Cancer Centre, Toronto, ON2; Princess Margaret Hospital, Toronto, ON3;
Toronto General Hospital, Toronto, ON4; Ottawa Regional Cancer Centre,
Ottawa, ON5;
118
1P, 19Q, 9P AND 10Q CHROMOSOMAL DELETIONS: THE IMPLICATIONS ON
TREATMENT RESPONSE IN PROGRESSED OLIGODENDROGLIAL
TUMOURS
Belinda Campbell1, Douglas Horsman1, John Maguire2, Sean Young1, Darko
Curman1, Roy Ma1, Brian Thiessen1
British Columbia Cancer Agency, Vancouver, BC1; Vancouver General Hospital,
Vancouver, BC2
119
EFFECT OF RADIOTHERAPY ON TUMOUR INTERSTITIAL ARCHITECTURE
AND FLUID DYNAMICS
Eric Leung1, Sarah Jane Lunt2, Richard P. Hill2, Michael Milosevic1
Princess Margaret Hospital, Toronto, ON1; University of Toronto, Toronto, ON2
120
HETEROGENEITY AND POWER IN CLINICAL BIOMARKER STUDIES
Melania Pintilie1, Vladimir Lakovlev2, Michael Milosevic1, David Hedley1,
Barbara Bachtiary3 Anthony Fyles1, Richard Hill1
Princess Margaret Hospital, Toronto, ON1; St. Michael’s Hospital, Toronto, ON2;
Medical University of Vienna, Vienna, Austria3
121
CLINICAL AND PRE-CLINICAL MEASURES OF RADIOSENSITIVITY IN MALE
BRCA1/2 CARRIERS RECEIVING RADIOTHERAPY
Danny Vesprini1, Farid Jalali1 John Trachtenberg2, Robert G. Bristow1
Princess Margaret Hospital, Toronto, ON1; University Health Network, Toronto,
ON2
122
COMBINATIVE EFFECTS OF GOLD NANOPARTICLES (GNPS) TRAIL AND
RADIATION ON GLIOMA MALIGNANT CELLS IN VITRO
Nadeem Pervez, Linghong Guo, James Xing, Kenneth Petruk, Nasser AlDhaibani, Amira Fitieh, Wilson Roa
Cross Cancer Institute, Edmonton, AB
123
ROLE OF 1–INTEGRIN EXPRESSION IN TRASTUZUMAB RESISTANCE
INRADIATION INDUCED HER-2-POSITIVE BREAST CANCER
John Thoms, Siham Sabri, David Lesniak, David Murray, Bassam Abdulkarim
Cross Cancer Institute, Edmonton, AB
124
VENTRICULAR ENLARGEMENT AND BRAIN VOLUME CHANGES POST
PROPHYLACTIC CRANIAL IRRADIATION IN SMALL CELL LUNG CANCER
PATIENTS
Valerie Panet-Raymond1, Paul S. Giacomini2, Raquel DelCarpio2, Mishkin
Derakhshan2, Alexandra Fiocco1, D. Louis Collins2, Douglas L. Arnold2, Luis
Souhami1, George Shenouda1
McGill University Health Centre, Montreal, QC1; Montreal Neurological Institute,
Montreal, QC2
125
DETECTION OF CIRCULATING TUMOUR CELLS FROM PERIPHERAL
BLOOD SAMPLES BY A NOVEL CELL CONCENTRATION METHOD AND
IMMUNOHISTOCHEMISTRY (IHC) IN STAGE III-IV NON-SMALL CELL LUNG
CANCER PATIENTS (NSCLC)
Caroline Chung1, Carrie Peters2, Chad Malloff3, Catherine Zaborowska4, Bryan
Knight1, Robert O'Connor5, Wan Lam3, Finbarr Sheehan1
British Columbia Cancer Agency, Vancouver, BC
1:30-2:20
Scientific Session 3 - The Micorenvironment Radiation Response
Moderators:
1:30-1:42
9
FUNCTIONAL CONSEQUENCES OF HYPOXIA-INDUCED DECREASES IN
HOMOLOGOUS RECOMBINATION
Norman Chan1, Marianne Koritzinsky2, Helen Zhao1, Stan Liu1, Carla
Coackley1, Ranjit Bindra3, Peter Glazer3, Simon Powell4, Abdellah Belmaaza5,
Brad Wouters2, Rob Bristow1
Ontario Cancer Institute, Toronto, ON1; GROW Research Institute, Maastricht,
Netherlands2; Department of Therapeutic Radiology, New Haven, CT3;
Department of Radiation Oncology, St. Louis, MO4; Institut du Cancer de
Montréal, Montréal, QC5
1:42-1:54
10
REGULATION OF GENE EXPRESSION IN HUMAN GLIOMA CELLS BY
HYPOXIA AND DCA
Joan Allalunis-Turner, Jason Derry
Cross Cancer Institute, Edmonton, AB
1:54-2:06
11
INFLUENCE OF MICROENVIRONMENT ON RADIATION-INDUCED
APOPTOSIS OF NEURAL PROGENITORS
Shun Wong, Fred Lu, Yuqing Li
Sunnybrook Health Sciences Centre, Toronto, ON
2:06-2:18
12
LACK OF HYPOXIA-INDUCED CARBONIC ANHYDRASE IX (CA IX)
EXPRESSION PREDICTS BETTER SURVIVAL IN WOMEN WITH SEROUS
EPITHELIAL OVARIAN CANCER TREATED WITH RADIOTHERAPY
J. Salvador Saldivar1, Ken Swenerton1, Steve Kalloger1, Blake Gilks2, Martin
Koebel2, Dianne Miller1, Jennifer Santos Santos1, David Huntsman1
British Columbia Cancer Agency, Vancouver, BC1; Vancouver General Hospital,
Vancouver, BC2
2:20-2:40
Break
2:40-3:40
Keynote Speaker
Molecular-targeted Agents and Radiotherapy
Bo Lu
3:40-4:40
Scientific Session 4 - Correlative Science and Biomarkers
Moderators:
3:40-3:52
13
VARIANTS IN DNA REPAIR GENES AND THEIR ASSOCIATION WITH LATE
SIDE-EFFECTS IN PROSTATE BRACHYTHERAPY PATIENTS
Mira Keyes1, J Pugh2, Tom Pickles3, James Morris1, Richard Moore1, B
Lorena2, D Thomas2,
C Yang2, Michael McKenzie1, Alex Agranovich1, MA Marra2
British Columbia Cancer Agency, Surrey, BC1; Michael Smith Genome Sciences
Centre, Vancouver, BC2; British Columbia Cancer Agency, Vancouver Cancer
Centre, Vancouver, BC3
3:52-4:04
14
ASSESSMENT OF MONOCYTE ACTIVATION MARKERS AND CIRCULATING
ENDOTHELIAL CELLS IN PATIENTS WITH LOCALIZED OR METASTATIC
BREAST CANCER
Michael Lock, David Goodale, Wendy Brown, Leslie Gray-Statchuk, Patricia
Furlong, Ian Chin-Yee, Michael Keeney, Alison Allan, Carolina Phay
London Regional Cancer Program, London, ON
4:04-4:16
15
PROGNOSTIC SIGNIFICANCE OF P16 IN LOCALLY ADVANCED SQUAMOUS
CELL CARCINOMA OF THE HEAD AND NECK TREATED WITH
CONCURRENT CISPLATIN AND RADIOTHERAPY
Harold Lau1, Desiree Hao1, Michael Eliasziw2, Susan Lees-Miller2, Anthony
Magliocco1
Tom Baker Cancer Centre, Calgary, AB1; University of Calgary, Calgary, AB2
4:16-4:28
16
HIGH ERCC1 EXPRESSION IS ASSOCIATED WITH WORSE SURVIVAL IN
PATIENTS WITH LOCALLY ADVANCED CERVICAL CANCER TREATED WITH
RADIOTHERAPY (RT): AN EVALUATION OF AQUA® VERSUS
CONVENTIONAL IHC METHODS
Corinne Doll1, Peter Craighead1, Roman Diaz1, Alan Box1, Brian Shin1, Michael
Eliasziw2, Susan Lees-Miller2, Anthony Magliocco1
Tom Baker Cancer Centre, Calgary, AB1; University of Calgary, Calgary, AB2
4:28-4:40
17
A PHASE I STUDY OF THE ANTI-EGFR ANTIBODY NIMOTUZUMAB
COMBINED WITH PALLIATIVE THORACIC RADIATION FOR PATIENTS WITH
ADVANCED NON-SMALL CELL LUNG CANCER
Anthony Brade1, Gwyn Bebb2, Colum Smith3, Stewart Rorke4, Igor Sherman5
Princess Margaret Hospital, Toronto, ON1; Tom Baker Cancer Centre, Calgary,
AB2; Hunter New South Wales Area Cancer Services, New South Wales,
Australia3; H. Michael Bliss Cancer Centre, St. John's, NL4; YM Biosciences,
Mississauga, ON5
4:40-5:30
Round Table Discussion - Biomarkers for Radiotherapy Clinical Trials
Corinne Doll
Andrew Minchinton
Bo Lu
Anthony Brade
5:30-5:40
Closing Remarks and Evaluation
Robert Bristow