Expression of the transcription factor, TFII-I, during post-implantation mouse
embryonic development, Fijalkowska I, Sharma D, Bult C, Danoff SK
Additional File 3: Developmental Expression of TFII-I mRNA and Reported
Expression of TFII-I in Mouse Development
In order to expand on the available description of timing and location of the
expression of TFII-I documented in the literature [1-3] we reviewed a number of
resources on gene expression profile. Recognizing that each resource has some
limitations, we sought to gain an overview of the expression pattern of TFII-I and its
relationship with the related factor, TFII-IRD1, in development. Profiles of expressed
sequence tags (ESTs) available from NCBI [4] demonstrate TFII-I mRNA expression in
pre-implantation cDNA libraries (Table 3a). In comparison with TFII-IRD1, TFII-I
message appears more abundant (based on transcripts per million) and is detected at
any earlier stage. TFII-I message is detected throughout development and adult life,
but expression is maximal during embryonic development.
To further delineate early expression, we reviewed data from The Jackson
Laboratory on Mouse Gene Expression Database [5, 6] and EMAGE—Edinburgh
Mouse Atlas of Gene Expression [7], summarized in the Table 3b. In agreement with
EST expression profile is the observation that trace or low expression levels were
detected in an early stage until E3.5 [8]. Other authors, however, detected TFII-I by RTPCR in egg [3]. Immunohistochemical studies demonstrate presence of TFII-I protein in
trophectoderm and deciduas at E4.5 during the pre-implantation period. In situ
1
hybridization studies focused primarily on the central nervous system, detailing a
complex pattern of regional expression at E13.5 and post-natal day 0 [9]. In situ
hybridization studies in E14 mouse embryo suggested ubiquitous RNA expression, but
with regional variation in intensity [10].
Table 3a. Expression profiles of TFII-I and TFII-IRD1 by analysis of EST counts. Data
extracted from EST Profile Viewer (NCBI) [4, 11]. Developmental stages are defined as
noted. Numerical data represent the number of transcripts per million (TPM). These
numbers reflect a low-end estimate for the presence of a given transcript at a particular
developmental stage, but suggest that TFII-I and TFII-IRD1 are expressed earlier than
VEGFR-2.
DEVELOPMENTAL STAGE
TFII-I
(Mm.261570)
TFII-IRD1
(Mm.332735)
0
153
Pre-implantation
202
63
Post-implantation
255
34
Mid-gestation
342
69
Late-gestation
468
89
Neonate
168
102
Post natal
150
27
Adult
152
34
Egg
2
Table 3b. Reported Expression of TFII-I in Mouse Development.
DEVELOPMENTAL STAGE
STATUS
METHOD OF
DETECTION
REFERENCE
Unfertilized egg;
Maternally
expressed,
Present
Present
Indirect
immunofluorescence,
RT-PCR
[3]
Zygote: pronuclei;
2-cell stage: nuclei, cytoplasm; polar body
nucleus;
8-16-cell and morula: inner cell mass (ICM),
trophectoderm;
ED3.5, early blastocyst: ICM, trophoectoderm
E4.5 embryo: nuclei and cytoplasm of ICM,
and trophoblast cells. Decidual cells: nuclei
and cytoplasm
Present
Present
Present
Present
Oocyte, 2-cell to 8-cell embryo, morula;
ED3.5 blastocyst
ED4.5 blastocyst and ICM
Trace or weak
Moderate
Trace or weak
RT-PCT
ED3.5 ICM, trophoectoderm
Strong, present
ED8.5: diffused expression pattern
Present
In situ
hybridization,
LacZ staining of
gene-trap
embryos
[13]
ED10.5: central nervous sytem
Ambiguous
RNA in situ
[9]
ED12.5: embryo: developing jaw,
presumptive tooth regions
Present
In situ
hybridization
[14]
Present
RNA in situ
[9]
Present
RNA in situ
[9]
WISH
[8]
[12]
ED13.5: embryo; head, central nervous
system, cerebral cortex, corpus striatum,
thalamus, hypothalamus, midbrain,
hindbrain, ventricular layer, spinal cord,
P0: head, central nervous system, cerebral
cortex, cerebellum, corpus striatum,
hindbrain, spinal cord, cranial ganglion,
dorsal root ganglion, tooth.
3
References
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
LA Perez Jurado, YK Wang, R Peoples, A Coloma, J Cruces, U Francke: A
duplicated gene in the breakpoint regions of the 7q11.23 Williams-Beuren
syndrome deletion encodes the initiator binding protein TFII-I and BAP-135,
a phosphorylation target of BTK. Hum Mol Genet 1998, 7:325-34.
SK Danoff, HE Taylor, S Blackshaw, S Desiderio: TFII-I, a candidate gene for
Williams syndrome cognitive profile: parallels between regional expression
in mouse brain and human phenotype. Neuroscience 2004, 123:931-8.
B Enkhmandakh, N Bitchevaia, F Ruddle, D Bayarsaihan: The early embryonic
expression of TFII-I during mouse preimplantation development. Gene Expr
Patterns 2004, 4:25-8.
DL Wheeler, T Barrett, DA Benson, SH Bryant, K Canese, V Chetvernin, DM
Church, M Dicuccio, R Edgar, S Federhen, et al: Database resources of the
National Center for Biotechnology Information. Nucleic Acids Res 2008,
36:D13-21.
MGIWS Gene Expression Database (GXD), The Jackson Laboratory, Bar
Harbor, Maine. World Wide Web (URL: http://www.informatics.jax.org). [July
2005.]: In: Book. City.
CM Smith, JH Finger, TF Hayamizu, IJ McCright, JT Eppig, JA Kadin, JE
Richardson, M Ringwald: The mouse Gene Expression Database (GXD): 2007
update. Nucleic Acids Res 2007, 35:D618-23.
S Venkataraman, P Stevenson, Y Yang, L Richardson, N Burton, TP Perry, P
Smith, RA Baldock, DR Davidson, JH Christiansen: EMAGE--Edinburgh Mouse
Atlas of Gene Expression: 2008 update. Nucleic Acids Res 2008, 36:D860-5.
RP Guo G.: Transcription Factor Dynamics in the preimplantation embryo.
In: Book Transcription Factor Dynamics in the preimplantation embryo. City;
2008.
PA Gray, H Fu, P Luo, Q Zhao, J Yu, A Ferrari, T Tenzen, D-i Yuk, EF Tsung, Z
Cai, et al: Mouse Brain Organization Revealed Through Direct GenomeScale TF Expression Analysis. Science 2004, 306:2255-2257.
YK Wang, LA Perez-Jurado, U Francke: A mouse single-copy gene, Gtf2i, the
homolog of human GTF2I, that is duplicated in the Williams-Beuren
syndrome deletion region. Genomics 1998, 48:163-170.
EW Sayers, T Barrett, DA Benson, SH Bryant, K Canese, V Chetvernin, DM
Church, M DiCuccio, R Edgar, S Federhen, et al: Database resources of the
National Center for Biotechnology Information. Nucleic Acids Res 2009,
37:D5-15.
T Yoshikawa, Y Piao, J Zhong, R Matoba, MG Carter, Y Wang, I Goldberg, MS
Ko: High-throughput screen for genes predominantly expressed in the ICM
of mouse blastocysts by whole mount in situ hybridization. Gene Expr
Patterns 2006, 6:213-24.
B Enkhmandakh, AV Makeyev, L Erdenechimeg, FH Ruddle, NO Chimge, MI
Tussie-Luna, AL Roy, D Bayarsaihan: Essential functions of the WilliamsBeuren syndrome-associated TFII-I genes in embryonic development. Proc
Natl Acad Sci U S A 2009, 106:181-6.
4
14.
A Ohazama, PT Sharpe: TFII-I gene family during tooth development:
candidate genes for tooth anomalies in Williams syndrome. Dev Dyn 2007,
236:2884-8.
5