Lab 22 Kern Medical Center Laboratory Name________________________ 1. Describe the Biosafety level of the KMC lab and how they treat organisms that are from each level? a. Biosafety Level 1 b. Biosafety Level 2 c. Biosafety Level 3 d. Biosafety Level 4 2. Name the different types of incubators and the types of cultures incubated in each one at KMC (don’t forget the Blood Culture incubator) 3. When an anaerobic culture is brought to the lab, what special treatment does it receive? 4. What are the most common anaerobic pathogens isolated at KMC and what specimens do they come from? 5. What type of culture or culture source is the most common one cultured in the KMC lab? 1 6. Respiratory Cultures: How do they set up throat and sputum cultures at KMC? (What media and discs?) Culture Type Media Any Rapid Tests Main Organism Identified Throat Sputum BAL Tracheal Aspirate Other 7. Which organism is isolated most commonly from Respiratory Cultures? 8. What is the theory and accuracy behind a “Quick Strep Test”? 9. What is the most common organism isolated from Urine Cultures at KMC? 10. Once an organism is isolated from a urine culture, how do they identify it and do sensitivities? 2 11. What is the shortest period of time required to definitively identify an organism? 12. What is the principle behind the VITEK sensitivity and what is the shortest period of time required to get a sensitivity? 13. What is the difference between Beta Strep Group A and B on the BAP plate and clinically? 14. How does KMC interact with the Public Health Laboratory? 15. What is the source of the most common nosocomial infections? 16. How dies KMC culture and identify blood culture pathogens? 17. Within a year, how often does KMC isolate the following a. MRSA 3 b. VRSA c. VRE d. ERBL 18. Describe the use of ELISA or EIA testing at the KMC lab. (What is the theory and what is it used for.) 19. What are the most common STD-related organisms isolated at KMC? 20. What are the most exciting things James has ever experienced in the KMC microbiology laboratory? 21. How do you become a Medical Technologist? 4